Banca de DEFESA: SANDRA REGINA CARNEIRO DE ARAUJO FAGNANI

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : SANDRA REGINA CARNEIRO DE ARAUJO FAGNANI
DATE: 27/05/2022
TIME: 09:00
LOCAL: Plataforma Conferencia Web - RNP
TITLE:

EFFECTIVENESS OF ANTIPARISITARY PHOTOCHEMOTHERAPY WITH LASER LIGHT IN BALB/C MICE INFECTED BY Leishmania brasiliensis: HISTOLOGICAL STUDY OF LESIONS

KEY WORDS:

Inflammation; trypanosomatids; tropical Medicine; Photobiology.

PAGES: 59
BIG AREA: Outra
AREA: Tecnologia e Inovação
SUMMARY:

This study aimed to perform a histological evaluation in skin lesions caused by Leishmania braziliensis after PACT treatment using Laser associated with 1.9. dimethyl methylene blue BALB/c mouse ear infection model was used. A total of 40 animals were assigned into two groups considering time intervals at 5 and 10 weeks and subdivided into four subgroups: Control, Photosensitizer, Laser and PACT. Two therapeutic interventions were performed after the 5th week of infection at 48-h intervals. 1.9 Dimethyl methylene blue was used as a photosensitizer at the concentration of 7 ng/mL, with a non-invasive topical administration method associated with Laser (λ = 660 nm, 40 mW, 12 J/cm2). Sample collection occurred 5 or 10 weeks after therapeutic interventions. The main histological findings were observed in the laser and PACT groups at the 10-week evaluation. The Laser group showed reduced lymphoplasmacytic inflammation and histiocytes (p=0.0079). The PACT group showed reductions in lymphoplasmacytic inflammation at 5 and 10 weeks, discrete reduction of histiocytes and a higher percentage of tissue remodeling. PACT with non-invasive topical administration of the photosensitizer was able to reduce lymphoplasmacytic inflammation and increase tissue remodeling in leishmaniosis skin lesions. This protocol may be easily used in humans and clinical trial shall be carried out to confirm the animal’s findings.

BANKING MEMBERS:
Presidente - 1134693 - ANTONIO LUIZ BARBOSA PINHEIRO
Interno - 1227468 - JEAN NUNES DOS SANTOS
Externa à Instituição - JULIANA SANTOS DE CARVALHO MONTEIRO - UEFS
Interna - 1217990 - MARIA CRISTINA TEIXEIRA CANGUSSU
Externa à Instituição - SUSANA CARLA PIRES SAMPAIO DE OLIVEIRA - UniRuy