Banca de DEFESA: FELIPE PESSOA DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : FELIPE PESSOA DE OLIVEIRA
DATA : 30/10/2020
HORA: 09:00
LOCAL: Sala virtual - UFBA
TÍTULO:

ANTINEOPLASTIC POTENTIAL OF THE ESSENTIAL OIL FROM THE BARK OF Aniba parviflora (Meisn.) Mez (Lauraceae)

PALAVRAS-CHAVES:

Aniba parviflora; Cancer; HepG2 cells; Essential oil.

PÁGINAS: 50
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
SUBÁREA: Farmacognosia
RESUMO:

Introduction: Aniba parviflora (Meisn.) Mez is an aromatic tree belonging to the Lauraceae family and it is found in the Amazon region of several South American countries. Many pharmacological properties have been reported about the genus Aniba, however, there are few studies investigating its antineoplastic potential. Aim: To evaluate the anticancer potential of the bark essential oil of A. parviflora (EOAP). Methods: The EOAP cytotoxicity was tested in different cancer cell lines, HL-60 (human acute promyelocytic leukemia), MCF-7 (human breast carcinoma), HepG2 (human hepatocellular carcinoma), HCT116 (human colon carcinoma) and B16-F10 (mouse melanoma), and non cancer cell line, MRC-5 (human lung fibroblast) through the alamar blue assay after 72 h incubation. Next, HepG2 cells were incubated with the EOAP (5, 10 and 20 μg/mL) for 48 h, proceeding with cell cycle analysis by flow cytometry. CB-17 SCID mice were subcutaneously inoculated with HepG2 to assess the essential oil (40 and 80 mg/kg) in vivo antitumor potential. Results: Chemical analysis identified linalool (16.3 ± 3.15%), α-humulene (14.5 ± 2.41%) and δ-cadinene (10.2 ± 1.09%) as the major components present in the EOAP. The IC50 values displayed for EOAP ranged from 9.05 to > 50 μg/mL for HepG2 and HCT116 cells, respectively. The IC50 value found for the non-cancer cell MRC-5 was 30.46 μg/mL. The EOAP at a concentration of 20 μg/mL induced DNA fragmentation in HepG2 cells after 48 h incubation, suggesting cell death. In the in vivo xenograft study, it was demonstrated that mice presented reduced tumor weight after treatment with the highest dose of EOAP (80 mg/kg). Conclusion: The essential oil from the bark of A. parviflora might be a valuable source of new substances for cancer treatment, taking into account its cytotoxic and antitumor action.

MEMBROS DA BANCA:
Presidente - 846.758.293-68 - DANIEL PEREIRA BEZERRA - UFC
Interno - 1281669 - RENATA BIEGELMEYER DA SILVA RAMBO
Externo à Instituição - GARDENIA CARMEN GADELHA MILITÃO - UFPE