|
Dissertations |
|
1
|
-
MAIARA DE SOUZA OLIVEIRA
-
CYTOTOXIC ACTIVITY OF A NEW PLATINUM COMPLEX CONTAINING PIPLARTIN AS LIGENT
-
Advisor : DANIEL PEREIRA BEZERRA
-
COMMITTEE MEMBERS :
-
DANIEL PEREIRA BEZERRA
-
CAROLINE BRANDI SCHLAEPFER SALES
-
JUNIA RAQUEL DUTRA FERREIRA
-
Data: Jan 15, 2018
-
-
Show Abstract
-
Piplartine, also known as piperlongumine, is an alkaloid/amide found in plants of the genus Piper, which has been extensively studied for its cytotoxic properties. In addition, metal complexes, such as platinum complexes, are successfully used in the treatment of cancer. Thus, the objective of the present work was to study the cytotoxic properties of a new platinum complex containing piplartine as a binder (CPP01). The platinum complex CPP01 was synthesized and tested against different tumor cell types (HepG2, HL-60, HCT116, SCC9, HSC3, MCF7, K562 and B16-F10) and non-tumor (MCR5 and PBMC) cells by the alamar assay blue after 72 h of incubation. Subsequently, HL-60 cells were incubated for 24 and 48 h with CPP01 (1, 2 and 4 μM) and the number of viable cells was determined by the trypan blue exclusion assay. Cell morphology was assessed after staining with may-grunwald-giemsa and cell cycle analysis, mitochondrial transmembrane potential and labeling for annexin V/propidium iodide, reactive oxygen species were determined by flow cytometry. The activity of caspase-3 was determined by colorimetric assay, and DNA intercalation assay was performed by fluorescence method. The complex CPP01 showed IC50 values for tumor cells ranging from 0.95 to 6.82 μM for the HSC3 and MCF7 lines, which presented an IC50 value of 7.45 and 9.18 μM for non-tumor cells MCR5 and PBMC, respectively. Piplartine showed IC50 values for tumor cells ranging from 5.39 to 18.58 μM for the HCT116 and K562 lines, which presented an IC50 value of 17.34 and 34.22 μM for non-tumor cells MCR5 and PBMC, respectively. In trypan blue exclusion analysis, the complex CPP01 reduced the number of viable cells without increasing the number ofnon-viable cells. Typical morphology of apoptotic cell death, an increase in internucleosomal DNA fragmentation, reduction of mitochondrial transmembrane potential, induces oxidative stress and a marked labeling for annexin V and caspase-3 were observed complex-treated HL-60 cells, suggesting induction of apoptotic cell death. In conclusion, it is possible to verify that the complex CPP01 showed more potent cytotoxic activity than piplartine in different tumor cell lines, inducing oxidative stress and apoptotic cell death mediated by caspases in HL-60 cells.
|
|
2
|
-
MAIARA DE SOUZA OLIVEIRA
-
CYTOTOXIC ACTIVITY OF A NEW PLATINUM COMPLEX CONTAINING PIPLARTIN AS LIGENT
-
Advisor : DANIEL PEREIRA BEZERRA
-
COMMITTEE MEMBERS :
-
DANIEL PEREIRA BEZERRA
-
CAROLINE BRANDI SCHLAEPFER SALES
-
JUNIA RAQUEL DUTRA FERREIRA
-
Data: Jan 15, 2018
-
-
Show Abstract
-
Piplartine, also known as piperlongumine, is an alkaloid/amide found in plants of the genus Piper, which has been extensively studied for its cytotoxic properties. In addition, metal complexes, such as platinum complexes, are successfully used in the treatment of cancer. Thus, the objective of the present work was to study the cytotoxic properties of a new platinum complex containing piplartine as a binder (CPP01). The platinum complex CPP01 was synthesized and tested against different tumor cell types (HepG2, HL-60, HCT116, SCC9, HSC3, MCF7, K562 and B16-F10) and non-tumor (MCR5 and PBMC) cells by the alamar assay blue after 72 h of incubation. Subsequently, HL-60 cells were incubated for 24 and 48 h with CPP01 (1, 2 and 4 μM) and the number of viable cells was determined by the trypan blue exclusion assay. Cell morphology was assessed after staining with may-grunwald-giemsa and cell cycle analysis, mitochondrial transmembrane potential and labeling for annexin V/propidium iodide, reactive oxygen species were determined by flow cytometry. The activity of caspase-3 was determined by colorimetric assay, and DNA intercalation assay was performed by fluorescence method. The complex CPP01 showed IC50 values for tumor cells ranging from 0.95 to 6.82 μM for the HSC3 and MCF7 lines, which presented an IC50 value of 7.45 and 9.18 μM for non-tumor cells MCR5 and PBMC, respectively. Piplartine showed IC50 values for tumor cells ranging from 5.39 to 18.58 μM for the HCT116 and K562 lines, which presented an IC50 value of 17.34 and 34.22 μM for non-tumor cells MCR5 and PBMC, respectively. In trypan blue exclusion analysis, the complex CPP01 reduced the number of viable cells without increasing the number ofnon-viable cells. Typical morphology of apoptotic cell death, an increase in internucleosomal DNA fragmentation, reduction of mitochondrial transmembrane potential, induces oxidative stress and a marked labeling for annexin V and caspase-3 were observed complex-treated HL-60 cells, suggesting induction of apoptotic cell death. In conclusion, it is possible to verify that the complex CPP01 showed more potent cytotoxic activity than piplartine in different tumor cell lines, inducing oxidative stress and apoptotic cell death mediated by caspases in HL-60 cells.
|
|
3
|
-
Viviane de Sousa Moreira Almeida
-
Antimicrobial resistance in microbiota of the oral cavity
-
Advisor : JOICE NEVES REIS PEDREIRA
-
COMMITTEE MEMBERS :
-
JOICE NEVES REIS PEDREIRA
-
JAILTON DE AZEVEDO SILVA JUNIOR
-
PAULO JOSE LIMA JUIZ
-
Data: Feb 19, 2018
-
-
Show Abstract
-
Antimicrobial resistance may make it difficult to treat important diseases and the oral cavity plays a role in the spread of antibiotic resistant bacteria. The objective of this study was to evaluate the prevalence of resistance genes in the oral microbiota and its relation with caries and periodontal disease. The study was observational and prospective, characterized as cross-sectional type and was performed with individuals who sought care at the Bahia Dental School (FOUFBA) and the Faculty of Pharmacy of the Federal University of Bahia (LACFAR-UFBA), between October to November 2016. Were included 110 people of both sexes, over 18 years old, who agreed to participate in the study with a clinical examination, were interviewed through questionnaires containing personal and epidemiological data. A clinical examination of the volunteers was done and saliva and plaque collection were performed, which were stored in sterile collectors to be aliquoted and frozen at -70 ° C. Bacterial DNA was extracted from saliva and plaque samples using the Maxwell system ( Promega) and PCR were performed to identify the resistance genes: aac(6´),blaNDM, blaSPM, blaOXA-48, CTX-M, blaKPC, blaIMP, blaVIM, pbp-2b, mecA, erm(A), erm(B) e erm(C), blaTEM, blaSHV e nim. The program Epi-Ifo Windows v.3.5.3 (CDC, 2011) was used for storage and analysis of epidemiological data. The erm(B) gene was found in 58.2% (64/110), the blaTEM gene in 16.4% (18/110), the aac (6 ') gene in 1.8% (2/110), the mecA gene at 2.7% (3/110) and the pbp-2b gene at 1.8% (2/110). Resistance genes had a very similar prevalence in participants with caries and without caries, and in individuals with periodontal disease, they were more prevalent, especially in PSR score 2. However, it was not possible to establish a relationship between the presence of dental caries and the periodontal status of the study participants and the presence of resistance genes.
|
|
4
|
-
SILVIA SOUZA DE CARVALHO
-
Evaluation of the reactivity of specific IgG and IgA antibodies and nitric oxide levels in day care children infected with Giardia duodenalis
-
Advisor : MARCIA CRISTINA AQUINO TEIXEIRA
-
COMMITTEE MEMBERS :
-
MARCIA CRISTINA AQUINO TEIXEIRA
-
MARIA LUIZA BRITO DE SOUSA ATTA
-
RICARDO WAGNER DIAS PORTELA
-
Data: Feb 22, 2018
-
-
Show Abstract
-
Giardia duodenalis infection is common in poor countries and affects mainly children, with the majority of cases being asymptomatic. The trophozoites of Giardia duodenalis colonize the surface of the intestinal mucosa, stimulating local and systemic immune responses through their antigens. The infection induces the production of specific antibodies that can play a role in the elimination of the parasite, as well as being useful as a diagnostic tool to monitor the exposure to the infection. In addition to the humoral immune response, some mediators of the innate immune response, such as nitric oxide (NOx), may also be involved in protection against giardiasis. The main objective of this study was to analyze the reactivity of specific IgG and IgA antibodies and nitric oxide levels in children infected with Giardia duodenalis. This study evaluated 187 samples of serum, feces and saliva from children attending two daycare centers in the city of Salvador, Bahia. The fecal samples were examined by sedimentation by centrifugation to investigate enteroparasites, and by centrifugal fecal flotation in zinc sulfate method and coproantigen screening for diagnosis of G. duodenalis. The reactivity of seric IgG and IgA anti-Giardia antibodies was evaluated by an in-house immunoenzymatic method (ELISA) and the concentration of nitric oxide in serum and saliva were measured by the Griess method. The frequency of Giardia duodenalis infection in children was 8.5% (16/187); 12 cases were diagnosed by microscopy and coproantigens, and four cases were exclusively by the second, with high agreement between the methods (kappa = 0.84). The sensitivity and specificities of ELISAs were 80.0% and 90.0% for IgG, and 80.0% and 83.3% for IgA. Respectively, the reactivity of specific antibodies in the sera tested was 16% (30/187) for IgG and 28.9% (54/187) for IgA. No statistically significant differences were found in the nitric oxide dosagebetween groups, although a higher proportion of samples with high NOx concentrations (above 41μmol/L) were observed in both serum (25.0%) and saliva (33.3%) of children infected with Giardia duodenalis. Over 70% of the children were classified as eutrophic, with no differences between the Body Mass Index (BMI) and the parasitological positivity, or the reactivity of anti-Giardia duodenalis antibody. The high rate of anti-Giardia duodenalis IgG and IgA antibodies shows the high endemicity and early exposure to this protozoan in children, which may be useful as an auxiliary diagnostic tool. Due to limited sample size of parasitized children in this study, there was not possible to infer the relationship of serum and secretory NO levels with Giardia duodenalis infection.
|
|
5
|
-
TALITA NUNES DOURADO CARVALHO
-
EVALUATION OF ANTI-RODS AND ANTI-RINGS (ANTI-RR) ANTIBODIES IN PEOPLE WITH CHRONIC HEPATITIS C, PRE AND POST-TREATMENT
-
Advisor : MARIA LUIZA BRITO DE SOUSA ATTA
-
COMMITTEE MEMBERS :
-
MARCIA CRISTINA AQUINO TEIXEIRA
-
MARIA LUIZA BRITO DE SOUSA ATTA
-
RYAN DOS SANTOS COSTA
-
Data: Feb 28, 2018
-
-
Show Abstract
-
Hepatitis C virus (HCV) infection is a major cause of liver disease. Anti-rod and antiring (anti-RR) antibodies have been described as autoantibodies to antigenic targets that are key enzymes in nucleic acid and phospholipid biosynthesis, the CTPS1 (cytidina triphosphate synthase 1) and IMPDH2 (inosine monophosphate dehydrogenase 2). These autoantibodies have been associated with non-responders or relapses to treatment with interferon-α plus ribavirin (IFN/RBV). OBJECTIVES: To investigate the presence and frequency of anti-RR antibodies in patients with chronic hepatitis C (CHC), pre and post treatment, its relation to other autoimmunity markers, and associations with clinical and virological findings. PATIENTS, MATERIALS AND METHODS: Retrospective cohort study, including serum samples from 52 male and female individuals from the HUPES Hepatitis Clinic, clinically and serologically diagnosed for hepatitis C. The data from genotyping and staging of fibrosis in patients with CHC were obtained from medical records. The test of anti-RR antibodies was performed by indirect immunofluorescence (IIF) using as antigens Hep-2 cells, obtained from a commercial set of immunodiagnostic (INOVA Diagnostics). The autoimmunity markers, rheumatoid factor and cryoglobulins were investigated by nephelometry and cryoprecipitation, respectively. RESULTS: The prevalence of antiRR antibodies was 2% in untreated patients, 4% in the 12th week of treatment and 26% in the 24th week of treatment. No association was found between the presence of antiRR antibodies and clinical and virological parameters of the infection, but genotype 3 was associated with anti-RR in the 24th week of antiviral treatment. CONCLUSION: The presence of anti-RR antibodies is related to the duration of antiviral treatment with interferon-α plus ribavirin in hepatitis C.
|
|
6
|
-
VANESSA COSTA DOS SANTOS
-
RESPOSTA IMUNE ADAPTATIVA DE SUBCLASSES DE ANTICORPOS IgG NA HEPATITE C CRÔNICA
-
Advisor : AJAX MERCES ATTA
-
COMMITTEE MEMBERS :
-
AJAX MERCES ATTA
-
ANA LEONOR PARDO CAMPOS GODOY
-
ROBSON DA PAIXAO DE SOUZA
-
Data: May 16, 2018
-
-
Show Abstract
-
Introduction: Chronic hepatitis C virus infection affects millions of people living in different countries, including Brazil. Objective: To investigate the adaptive immune response mediated by subclasses of IgG antibodies to the recombinant core and NS3 antigens in patients with chronic hepatitis C. Methods: Sixty patients chronically infected with HCV genotype 1, without antiviral treatment, and 60 healthy subjects participated in the study. Serum levels of alanine aminotransferase, HCV viremia, the presence of cryoglobulinemia and liver fibrosis were determined. The presence of serum IgG1 and IgG4 antibodies against recombinant HCV NS3 core and non-structural protein antigens was investigated by indirect ELISA. Results: Anti-core and anti-NS3 IgG1 antibodies were detected in 33/60 (55%) and 46/60 (77%) of the patients, whereas two healthy controls reacted with one of the antigens (NS3). Anti-core IgG4 antibodies were not detected in both groups, while 30/60 (50%) of the patients had anti-NS3 IgG4 antibodies. Except for the observation of higher levels of anti-NS3 IgG4 antibodies in patients with low viremia (<8 x 105 IU/mL), IgG1 and IgG4 antibody levels were not influenced by ALT levels, the presence of cryoglobulinemia or degree of fibrosis hepatic. Conclusions: A vigorous production of anti-core and anti-NS3 IgG1 antibodies was found in chronic hepatitis C patients. In contrast, IgG4 antibodies seemed to be only produced to the NS3 non-structural antigen and appeared to participate in viremia control.
|
|
7
|
-
LARISSA MENDES BOMFIM
-
ANTINEOPLASTIC POTENTIAL OF NEW RUTENUM COMPLEX WITH 6-METHYL-2-THIOURACIL
-
Advisor : DANIEL PEREIRA BEZERRA
-
COMMITTEE MEMBERS :
-
DANIEL PEREIRA BEZERRA
-
DARIZY FLAVIA SILVA AMORIM DE VASCONCELOS
-
ROSANE BORGES DIAS
-
Data: May 28, 2018
-
-
Show Abstract
-
Ruthenium compounds have gained great interest because of their potent cytotoxicity in cancer cells, however, many of their potential applications remain unexplored. In this work, we investigated the antineoplastic potential of two new ruthenium complexes with 2-thiouracil (CR01 and CR02) in different cell models and their mechanisms of action in HL-60 human promyelocytic leukemia cells. The cytotoxicity of the complexes against different neoplastic lines was determined by the alamar blue assay. Subsequently, HL-60 human promyelocytic leukemia cells were incubated with CR01 and CR02 at concentrations of 1 and 2μM, and the number of viable cells was determined by the trypan blue exclusion assay. Cell cycle analysis, mitochondrial transmembrane potential and labeling for annexin V / propidium iodide were determined by flow cytometry. The caspase activation assay was also performed to evaluate the apoptotic processes induced by the complexes. The in vivo antitumor activity of the CR01 complex was evaluated in C.B-17 SCID mice inoculated with HL-60 cells. We found that CR01 and CR02 exhibited a potent cytotoxic effect on a panel of cancer cell lines. HL-60 cells treated with the complexes showed a reduction in the number of viable cells, increased fragmentation of internucleosal DNA, depolarization of mitochondrial transmembrane potential, activation of caspases 3, 8 and 9 and increased phosphatidylserine externalization, indicating induction of cell death apoptosis mediated by caspases. In addition, pretreatment of HL-60 cells with inhibitors of the major families of the MAPK signaling pathway (inhibitors of p38, JNK / SAPK and ERK1 / 2) prevented CR01 and CR02 induced apoptosis. In the in vivo model, CR01 inhibited the development of HL-60 cells in C.B-17 SCID mice, exhibiting a potent antitumor effect in vivo. These results indicate that ruthenium complexes with 2-thiouracil have anticancer potential under different carcinogenic strains, and induce caspase-mediated apoptotic cell death in HL-60 cells. In addition, the CR01 complex was able to inhibit its development in vivo.
|
|
8
|
-
ROUSILANDIA DE ARAUJO SILVA
-
Development of formulations for cutaneous moisturizing by cold emulsification: evaluation of sensorial properties.
-
Advisor : NEILA DE PAULA PEREIRA
-
COMMITTEE MEMBERS :
-
ADEMIR EVANGELISTA DO VALE
-
ANDRE LUIS MORAIS RUELA
-
NEILA DE PAULA PEREIRA
-
Data: May 30, 2018
-
-
Show Abstract
-
Skin products occupy the eighth position in the ranking of the most consumed cosmetics, and moisturizers represent the most important class of this group, since they play an important role in the prevention of xerosis and skin aging. Emulsion is the most used pharmaceutical form for topical products and their preparation requires energy, time and equipment expenditures, so the cold emulsification process minimizes the costs of the production process. In this context, the present study aims to develop cold emulsified formulations with the aid of the Gelaid CPE CF ® silicon base using vegetable oils of Sweet Almond (GA), Gueroba (GGE) and Macaúba (GM) and Urea (GU) moisturizing active ingredients. Oleate Lactate (GL), Octadecila Lactate (GO) and Urea Glycospheres (GG) evaluating their sensory properties. The study was divided into three stages. The first one was the study of the Stability of the Formulations following its behavior during the 90 days by means of the macroscopic evaluation, analysis of the pH, Spreadability in vitro. All formulations remained stable without change in their organoleptic characteristics and remained within the cutaneous pH. In the group of vegetable oils the GM formulation (Eimax 3739.8mm2) presented better spreadability performance and in the group with active moisturizing the GO formulation (Eimax 2687.95 mm2). The second stage was the sensorial characterization through the determination of the texture profile and the rheological behavior. In the texture, the GGE formulation recorded lower values of firmness (4.73 g) and in the group with active the GO formulation (4.63 g). The rheological evaluation determined that all formulations presented pseudoplastic behavior with thixotropy and better recovery for GM and GO formulation in their respective groups. The last step of the study was the sensory analysis by the preference method in which the formulations with the highest score, in the case of GGE oils (44 points) and the active ones at GO (60 points) were considered preferred as regards spreadability by the research participants. The results found in the formulations with oil and moisturizing active demonstrate the feasibility in developing moisturizing products by the process of cold emulsification with different raw materials.
|
|
9
|
-
ROUSILANDIA DE ARAUJO SILVA
-
Development of formulations for cutaneous moisturizing by cold emulsification: evaluation of sensorial properties.
-
Advisor : NEILA DE PAULA PEREIRA
-
COMMITTEE MEMBERS :
-
ADEMIR EVANGELISTA DO VALE
-
ANDRE LUIS MORAIS RUELA
-
NEILA DE PAULA PEREIRA
-
Data: May 30, 2018
-
-
Show Abstract
-
Skin products occupy the eighth position in the ranking of the most consumed cosmetics, and moisturizers represent the most important class of this group, since they play an important role in the prevention of xerosis and skin aging. Emulsion is the most used pharmaceutical form for topical products and their preparation requires energy, time and equipment expenditures, so the cold emulsification process minimizes the costs of the production process. In this context, the present study aims to develop cold emulsified formulations with the aid of the Gelaid CPE CF ® silicon base using vegetable oils of Sweet Almond (GA), Gueroba (GGE) and Macaúba (GM) and Urea (GU) moisturizing active ingredients. Oleate Lactate (GL), Octadecila Lactate (GO) and Urea Glycospheres (GG) evaluating their sensory properties. The study was divided into three stages. The first one was the study of the Stability of the Formulations following its behavior during the 90 days by means of the macroscopic evaluation, analysis of the pH, Spreadability in vitro. All formulations remained stable without change in their organoleptic characteristics and remained within the cutaneous pH. In the group of vegetable oils the GM formulation (Eimax 3739.8mm2) presented better spreadability performance and in the group with active moisturizing the GO formulation (Eimax 2687.95 mm2). The second stage was the sensorial characterization through the determination of the texture profile and the rheological behavior. In the texture, the GGE formulation recorded lower values of firmness (4.73 g) and in the group with active the GO formulation (4.63 g). The rheological evaluation determined that all formulations presented pseudoplastic behavior with thixotropy and better recovery for GM and GO formulation in their respective groups. The last step of the study was the sensory analysis by the preference method in which the formulations with the highest score, in the case of GGE oils (44 points) and the active ones at GO (60 points) were considered preferred as regards spreadability by the research participants. The results found in the formulations with oil and moisturizing active demonstrate the feasibility in developing moisturizing products by the process of cold emulsification with different raw materials.
|
|
10
|
-
LAURA MARIA SANTOS DO NASCIMENTO
-
APPLICATION OF SEED OIL AND EXTRACT FROM LEAVES Psidium guajava L. IN EMULSIFIED SYSTEMS WITH COSMETIC POTENTIAL
-
Advisor : NEILA DE PAULA PEREIRA
-
COMMITTEE MEMBERS :
-
FABIO ROCHA FORMIGA
-
NEILA DE PAULA PEREIRA
-
RENATA BIEGELMEYER DA SILVA RAMBO
-
Data: Jun 12, 2018
-
-
Show Abstract
-
The guava (Psidium guajava L.) is a tropical fruit of great prominence, with high levels of vitamin C, phenolic compounds, carotenoids, besides having high concentration of vegetable oil, being potential for use in cosmetic formulations as active and/or component of the oil phase of emulsifying systems. In this context, the objective of this work was to obtain O/W emulsions incorporated with fixed oil, obtained from guava seeds, and the hydroethanolic extract of the leaves of P. guajava L., Paluma cultivar, claiming to obtain a cosmetic formulation moisturizing and antioxidant. Initially, the fixed oil of the guava seeds was obtained in a Soxhlet device using hexane, and the derivatized fatty acids were characterized by gas chromatography coupled to mass spectrometry. The physico-chemical properties of the oil were evaluated for relative density, peroxide index, acidity index, saponification index and iodine index. In the second stage the hydroethanolic extract of the leaves of P. guajava was obtained by the maceration method followed by percolation, being evaluated the pH and the relative density of the same. The liquid extract was concentrated by atomization in spray dryer and from the dry extract, the water activity, the quantification of the total phenolic compounds and the antioxidant activity by the free radical sequestration DPPH• were determined. In addition to the hydroethanolic extract and fixed seed oil, the in vitro cytotoxicity assay was performed in murine macrophages of the J774 balb/c lineage, using the colorimetric method of resazurin reduction (Alamar Blue®), in addition to thermogravimetric analysis to evaluate the thermal stability through the TG/DTG curves. Afterwards, the ionic and non-ionic emulsions incorporated with the fixed oil of the seeds were obtained, being submitted to the test of analysis of the organoleptic and microscopic characteristics, pH determination, accelerated stability, in vitro spreadability and texture profile during 90 days. Based on the performance of the formulations, new non-ionic emulsions containing 2.5%, 5.0% and 7.0% of the hydroethanolic extract were prepared. The antioxidant activity was analyzed as a criterion for the development of an emulsion containing 2, 5% of fixed seed oil and hydroethanolic extract of guava leaves. The emulsion containing oil and extract was evaluated for the antioxidant potential. The results showed a predominance of unsaturated fatty acids for the fixed seed oil, with linoleic acid predominantly (80.41%). As for the physico-chemical properties, the oil was in accordance with the literature, making it potentially applicable in the cosmetics industry. As for the extract the relative density was within expected range and the pH was slightly acidic. The water activity of the dry extract was 0.265 (Aw), which does not favor the growth of microorganisms. The total phenolic content was 127.78 ± 2.87 mg gallic acid /g extract. The antioxidant activity of the ethanolic extract was 89.15 ± 0.29% and EC50 of 0.1008 mg/mL, these results being promising for the development of innovative cosmetics. In the thermal stability evaluation the seed oil was stable at a temperature of 360ºC, the leaves extract is stable below 45ºC. The oil and extract were not cytotoxic to the cell line tested until the concentration of 10 µg/mL. The obtained emulsions were stable during the period evaluated, and the pH did not suffer a marked variation. Regarding the spreadability and texture, the non-ionic emulsions presented better performance, where the emulsion containing the fixed oil of the seeds was distinguished by maintaining the behavior during the 90 days. For the emulsions containing the hydroethanolic extract, antioxidant activity was observed, varying from 45.88 ± 1.11 to 64.58 ± 0.93%. The emulsion, increased 2.5% of fixed seed oil and hydroethanolic extract of the leaves presented promising results of antioxidant activity of 54.89 ± 1.53%, opening the possibility for the development of an innovative phytocosmetics with antioxidant and moisturizing activity.
|
|
11
|
-
CÁSSIA VARGAS LORDÊLO
-
FREQUENCY OF C677T, G1691A AND G20210A POLYMORPHISMS IN THE GENES OF METHYLENETETRAHYDROPHOLATE REDUTASE, FACTOR V E PROTROMBINE IN CAROTYDE DISEASE
-
Advisor : RICARDO DAVID COUTO
-
COMMITTEE MEMBERS :
-
RICARDO DAVID COUTO
-
ROQUE ARAS JUNIOR
-
CYNARA GOMES BARBOSA
-
Data: Jul 25, 2018
-
-
Show Abstract
-
In recent years, molecular targets are being direct and indirect associated with risk markers in the development of cardiovascular diseases. In this context, the study objective was to determine the MTHFR enzyme gene polymorphism C677T, Factor V gene G1691A and Prothrombin gene G20210A frequency’s, and to investigate the role of risk biomarkers in the development of carotid artery disease (CAD). For this purpose, DNA extraction was performed from the whole blood samples of 62 patients, militaries, and their dependents, from the Hospital Naval de Salvador, Bahia. The collected DNA samples were submitted to PCR, followed by enzymatic digestion (RFLP). The associations between CAD, biomarkers and genetic polymorphisms were measured by Fisher's test. The mean age of participants was 57.85 ± 15.74, ranging from 20 to 77 years. Among individuals who underwent carotid doppler sonography, 46.8% did not show CAD, and 53.2% had some degree of atherosclerosis in the carotid artery. The frequency of Factor V G1691A polymorphism in the study population was 1.6%, for heterozygous genotype (GA). The G20210A polymorphism in the Prothrombin gene showed the frequency of 3.2% of heterozygosity (GA), and the MTHFR enzyme gene C677T polymorphism was 24.2% for heterozygotes (CT), and 1.6% for homozygotes (TT). The results suggested that there is no association between the MTHFR enzyme gene C677T polymorphism and the CAD in the studied population and the Plasma Atherogenic Index (AIP) has been shown to be a good parameter for the associated diagnostic assistance of subclinical CAD risk, mainly in females.
|
|
12
|
-
PAULA SCHONS VIECELI
-
THERAPEUTIC POTENTIAL OF PHYSALIS ANGULATA EXTRACT RICH IN PHYSALINES IN INDUCED PERIODONTAL DISEASE MODEL
-
Advisor : CRISTIANE FLORA VILLARREAL
-
COMMITTEE MEMBERS :
-
CRISTIANE FLORA VILLARREAL
-
FRANCINE JOHANSSON AZEREDO
-
TERCIO CARNEIRO RAMOS
-
Data: Jul 26, 2018
-
-
Show Abstract
-
Periodontal disease (PD) is a disease with one of the greatest risks for oral health, and triggers tooth loss if left untreated. Fisalines are dry-steroids with anti-inflammatory and immunomodulatory properties. Aiming to establish the scientific basis for herbal medicine future development, this paper evaluated the therapeutic potential of physalis angulata extract rich in fisalines (EPA) in animal model of periodontal disease. PD was induced by injections of LPS 20 μg / 1μl for 28 days. Treatments consisted of daily oral administrations of EPA 50 or 100 mg / kg, vehicle (saline + 5% DMSO) or nimesulide (25 mg / kg, reference drug) during 14 days. The jaws and gingiva were collected to measure bone loss, histology, PCR and ELISA. Stomach, kidneys, liver, heart and blood were collected for toxicity testing. The results showed that EPA (50 and 100 mg / kg) was able to inhibit alveolar bone loss when compared to vehicle (p <0.05). Treatment with EPA (50 and 100 mg / kg) inhibited expression of mRNA and proinflammatory cytokines IL-1β and IL-6 (p <0.05), but not TNF-α. An increase in the anti-inflammatory cytokine TGF-β (p <0.05) was noted, but not in IL-10. EPA reduced the expression of MMP-9, but not TIMP-1, in gingival tissue (p <0.05).The histopathological analysis did not show any changes in the experimental groups. The analysis of systemic toxicity did not report statistically significant differences in blood parameters between groups treated with EPA and vehicle. On the other hand, treatment with nimesulide altered the RBC (p <0.05), HGB (p <0.05), HCT (p <0.05) and MCHC (p <0.05) parameters compared to vehicle. Biochemical parameters have not changed. A histological analysis of the stomach indicated histopathological changes in all treated groups in compered to naive, including presence of ulcer, necrosis and hemorrhage.This study demonstrated that EPA has therapeutic properties in PD and may represent a promising coadjutant approach in the treatment of that disease. On the other hand, the gastric toxicity evidenced needs a better investigation.
|
|
13
|
-
Homegnon Antonin Ferreol Bah
-
Influence of the enzyme delta-aminolevulinic acid dehydratase on the association between plumbemia and children's intellectual function
-
Advisor : JOSE ANTONIO MENEZES FILHO
-
COMMITTEE MEMBERS :
-
JOSE ANTONIO MENEZES FILHO
-
FERNANDO MARTINS CARVALHO
-
EDNA LUCIA SANTOS DE SOUZA
-
Data: Jul 31, 2018
-
-
Show Abstract
-
Introduction: Lead (Pb) is a ubiquitous environmental contaminant which affects children cognition and inhibits activity of the delta-aminolevulinic acid dehydratase (enzyme ALAD). The community of Maragogipinho, (district of Aratuípe) is exposed to Pb due to the glazed ceramics produced without protective measures. Objective: This work aimed to evaluate the association between Pb exposure and the intellectual level of children considering the interaction with the enzyme ALAD. Methods: Children of 5.5 to 13 years from two areas (four schools) of Maragogipinho, previously classified as low exposure (LEx; n = 34) and Moderate Exposure (MEx; n = 40) were compared with children (n = 69) of the urban center of the municipality considered as control (CG). The blood lead level (BLL) and Pb loading rate of (RtPb) in settled dust of the children residences (n = 108) were determined. The nutritional status (z-score A / I), hemoglobin concentration (Hb), iron deficiency (Ferritin), activity and polymorphism of the enzyme ALAD were evaluated. The questionnaire HOME was applied, while the non-verbal intelligence of the children and mothers were evaluated using the Raven’s Progressive Matrices. Results: The median (range) of RtPb in settled dust from residence house was 65 (6 - 1964) μg Pb / m2 /30 days. The median (range) of BLL was 1.0 (0.1-21.3) μg/dL and was not influenced by the sex and socioeconomic status. The mean (SD) of Hb, ALAD, ferritin, z-score H/A, and raw score of Raven were respectively 13.2 (0.93) g/dL; 71 (31 - 113) U/L; 27.1 (3.1 - 152.8) ng/mL; 0.03 (-2.6 - 4.7) and 19.3 (5.6) while the phenotypes of the enzyme ALAD were ALAD 1/1 (97.9%) and ALAD 1/2 (2.1%). Spearman correlation confirmed the relationship of children BLL with the RtPb in settled dust from their homes (rho = 0.368, p <0.001), inhibition of ALAD enzyme activity by Pb (rho = -0.587; p ˂0.001). The prevalence rates of high blood lead (>5 μg/dL) in the LEx and MEx areas when compared to the CG were 1.5 and 3.7 respectively, indicating a higher risk of children residing in the area near the pottery facilities. No significant association was found between BLL and the Raven score, but its correlation with the activity of the ALAD enzyme (rho = -0.205; p = 0.021; n = 127) suggested the neuroprotective role of that enzyme. Conclusion: From this investigation, it can be concluded the existence of moderate exposure of children in this community, confirmed the hematological toxicity of Pb, the role of dust in children contamination. Among the main determinants of children BLL are: living in the vicinity of pottery facilities and involvement in the production of ceramics. The failure to observe the association between BLL and children's nonverbal intelligence, probably due to the lack of sensitivity of the neuropsychological instrument used, suggesting to further the investigation with more adequate cognitive tests.
|
|
14
|
-
MARCOS CUSTÓDIO FIUZA
-
S447X POLYMORPHISM ASSOCIATION IN THE LIPASE ENZYME GENE LIPOPROTEIC WITH CARDIOVASCULAR RISK MARKERS IN DISEASE CAROTIDE
-
Advisor : RICARDO DAVID COUTO
-
COMMITTEE MEMBERS :
-
ANA PAULA CAIRES DOS SANTOS VALVERDE
-
JOICE NEVES REIS PEDREIRA
-
RICARDO DAVID COUTO
-
Data: Aug 3, 2018
-
-
Show Abstract
-
In recent years, new biomarkers suggestive of cardiovascular risk are being tested for the associated diagnosis of atherosclerotic disease. Thus, the objective of this study was to identify the presence of LPL enzyme gene S447X (C1595G) polymorphism and associate its presence with imaging data and serum biomarkers in patients with and without carotid disease (CAD). The study sample population was composed of 111 patients treated at the Hospital Naval de Salvador; mean age 59.6 ± 13.9 years; all participants had whole blood DNA extraction, from that population, only 62 patients, mean age 57.85 ± 15.74 years, had carotid doppler performed. Then, PCR reaction followed by restriction endonucleases digestion (RFLP) were performed. Among patients who underwent carotid doppler sonography, 46.8% did not show CAD and 53.2% showed CAD. The frequency of the polymorphism was 81.98% for the wild homozygous (CC); 17.12% for heterozygote (CG); and 0.90% for mutant homozygote (GG). Individuals with the polymorphism exhibited significantly higher values for LDL-c (p = 0.0201) and atherogenic coefficient (p <0.0001) with the same trend for non-HDL-c (p = 0.0767) and for TG/HDL-c ratio (p = 0.0719). The frequency of the S447X polymorphism in the study population corroborated with that found in the literature, but its presence was not associated with the prevalence of CAD and with the comorbidities observed, such as diabetes mellitus, dyslipidemia and systemic arterial hypertension. AIP and other atherogenic indices can be used as discriminators of CAD.
|
|
15
|
-
RAIANA DOS ANJOS MORAES
-
ITACONIMIDATED DERIVATIVES INDICATE RELAXATION IN ARTERY MESSENTRICS AND NEGATIVE INOTROPISM IN RATS BY INHIBITION OF Ca2 + Influx savior
-
Advisor : DARIZY FLAVIA SILVA AMORIM DE VASCONCELOS
-
COMMITTEE MEMBERS :
-
DARIZY FLAVIA SILVA AMORIM DE VASCONCELOS
-
DANIEL PEREIRA BEZERRA
-
FABIANO ELIAS XAVIER
-
Data: Aug 20, 2018
-
-
Show Abstract
-
Introduction: Hypertension is a risk factor for various cardiovascular and renal diseases, representing a major public health challenge. Thus, investigating new substances with antihypertensive potential becomes necessary. In this way, analogues were synthesized, N-4-methyl-phenyl-itaconimide (3), N-4-methoxy-phenyl-itaconimide (6), N-phenyl-itaconimide (9) and N-4-chloro-phenyl-itaconimide (15), from unprecedented alkaloid, phylantimide, available in Phyllanthus sellowianu. Objectives: To evaluate the effects of cyclic imides on cardiovascular system and to investigate possible mechanisms of action involved in observed responses. Methods: In vitro studies, Wistar rats were euthanized in CO2 chamber and superior mesenteric artery and atria were removed. In mesenteric arteries were performed the measurement of isometric tension, in atria were evaluated the heart rate and force of cardiac contraction, and in vivo studies was evaluated the actions of imide 6 on blood pressure and heart rate CEUA/UFBA (nº120/2017). Results: Cumulative administration of cyclic imides (3x10-8 to 3x10-4M) in pre-contracted rings with phenylephrine, 1μM, induced vasorelaxation that was independent of endothelium-derived relaxing factors. Additionally, imide 9 presented higher potency in relation to imide 3, 6 and 15 and not statistical difference was observed among the maximum efficacies of the imides. The cumulative administration of imides to basal tonus did not alter vascular intrinsic tone. In addition, imide 6 induced vasorelaxation in rings exposed to depolarizing tyrode solution with 60 mM KCl or 20 mM KCl similar to the control, suggesting the non-participation of K+ channels. Imide 6 was able to attenuate the influx of Ca2+ in a concentration-dependent manner. As well as, imide 6 attenuated the CaCl2-induced contraction in nominally calcium-free medium in the presence of cyclopiazonic acid (20 μM), phenylephrine (1 μM) and nifedipine (1 μM), indicating an attenuation of the influx of Ca2+ by receptor-operated channel (ROC) and store-operated channel (SOC). The presence of SKF 96365 (10-5M), SOC blocker, did not significantly alter the vasodilatory effect induced by imide 6. In addition, imida 6 induced negative inotropic effect without significant change in cardiac rhythmicity. In vivo studies, in non-anesthetized normotensive rats, imide 6 lowered blood pressure and induced bradycardia. Conclusion: These results suggest that imides have concentration-dependent vascular effects and the vasorelaxation seems to be independent of endothelium-derived relaxing factors. The vasodilatory effect induced by imide 6 may be due to a possible influence on the CaV and ROC channels. In addition, imide 6 is able to reduce force of cardiac contraction, blood pressure and promote bradycardia.
|
|
16
|
-
ALAN OLIVEIRA DUARTE
-
SOROLOGICAL PROFILE FOR ZIKA VIRUS INFECTION PARENTS AND NEWBORN IN ONE MATERNITY OF REFERENCE OF SALVADOR-BA
-
Advisor : FERNANDA WASHINGTON DE MENDONCA LIMA
-
COMMITTEE MEMBERS :
-
FERNANDA WASHINGTON DE MENDONCA LIMA
-
LUCIANA SANTOS CARDOSO
-
RICARDO RICCIO OLIVEIRA
-
Data: Aug 27, 2018
-
-
Show Abstract
-
In 2015, the first cases of zika virus (ZIKV) infection in Brazil were confirmed. After six months, cases of microcephaly associated with the outbreak occurred, which occurred concomitantly with outbreaks of dengue (DENV) and chikungunya (CHIKV). Studies are needed to understand the consequences for our population of this contact with the triple epidemic, especially in the pregnant population. Our objective was to investigate the serological profile for these arboviruses in puerperal women and newborn infants attended at a maternity hospital in Salvador, Bahia, Brazil in 2016. We performed a cross-sectional, seroepidemiological study that evaluated the presence of antibodies in mothers attended by the maternity ward who presented exanthematic disease in pregnancy and her neonates. ELISA diagnostic kits (Euroimmun™) were used to detect IgM and IgG against ZIKV, DENV and CHIKV, and a standardized anti-ZIKV-specific IgM capture ELISA was used. 101 mothers were included in the study. Regarding seroprevalence, for anti-ZIKV IgM, 07 mothers (6.9%) and 04 neonates (4%) were reactive. For anti-CHIKV IgM, 23 mothers (22.8%) and 04 neonates (4%) were reactive. For anti-DENV IgM, 12 mothers (11.9%) tested positive. Of the 101 mothers, 73 (72.3% of the total), 39 (38.6%) and 93 (92.1%) were seropositive for IgG against ZIKV, DENV and CHIKV, respectively. Of the 102 neonates, 06 (5.9%) had microcephaly. We generated a new knowledge about the rates of congenital infection by ZIKV and CHIKV, and how these are expressed among mothers who presented exanthematous disease. Knowledge of seroprevalence for arboviruses is important to understand the extent of the arbovirus epidemic in Brazil, to evaluate groups susceptible to severe forms of disease and to stimulate prevention measures directed at these groups.
|
|
17
|
-
CATARINA MILENA MONTEIRO DA COSTA
-
INVESTIGATION OF THE REGENERATIVE THERAPEUTIC PROFILE OF THERAPY CELLULAR IN EXPERIMENTAL TRIGEMINAL NEURALGIA
-
Advisor : CRISTIANE FLORA VILLARREAL
-
COMMITTEE MEMBERS :
-
CRISTIANE FLORA VILLARREAL
-
DANIEL PEREIRA BEZERRA
-
PAULO JOSE LIMA JUIZ
-
Data: Aug 31, 2018
-
-
Show Abstract
-
INTRODUCTION: Among the various modalities of orofacial neuropathic pain, trigeminal neuralgia (NT) is the most common. It is associated with a variety of pathological conditions. The currently available treatments for trigeminal neuralgia, whether pharmacological or surgical, are not effective, highlighting the need to develop new therapeutic approaches for this syndrome. An emerging strategy is the use of stem cells. The therapeutic potential of these cells comes from their multiple sources of production, their mechanism of action and their regenerative potential. Stem cells have antinociceptive effect in trigeminal neuralgia, however the mechanisms involved in these effects are not yet well understood. The antinociceptive effect of stem cells in trigeminal neuralgia has been demonstrated, however, the mechanisms involved in these effects are not yet well understood. AIM: The purpose of this study was to evaluate the therapeutic potential of bone marrow mesenchymal stem cells (BMSC) and bone marrow mononuclear cells (CMMC) in the experimental model of trigeminal neuralgia, comparing their effects to those of pharmacological therapy and investigating their mechanisms of action. METHODS: BMSC were obtained from C57B16 mice and were characterized by flow cytometry and cell differentiation. Male C57Bl / 8 mice underwent surgery for induction of the neuropathy model by partial ligation of the infraorbital nerve and during 30 days were evaluated for body weight and thermal and mechanical nociceptive thresholds in the hargreaves and von Frey tests, respectively. Five days after induction of the model, the animals received single intravenous administration of CMMC (1x106), BMSC (1x106) or vehicle (100μ). The effects of cell therapy on the cytokine profile and structural alterations in the infraorbital nerve were investigated 10 and 30 days after the treatments, by ELISA and 30 (30 mg / kg), used as reference pharmacological treatment, by intraperitoneal route. days by optical and electron microscopy, respectively. RESULTS: BMSC and CMC transplantation completely reversed the thermal hyperalgesia (p <0.001) and mechanical allodynia (p <0.001) of neuropathic animals throughout the experimental period. As expected, carbamazepine induced short-term antinociceptive effect. The quantitative analysis of IL-1β, TNF-α, IL-1 cytokines in the infraorbital nerve of the mice showed that CMMO or CMsMO reduced IL-1β, TNF-α (p <0.001), and increased levels of IL-10 (p <0.001). Supporting the observed long-term antinociceptive effect, cellular therapy reduced structural alterations in the infraorbital nerve of neuropathic animals, with the reduction of axonal degeneration in myelinic and myelinic fibers and mitochondrial atypia. CONCLUSION: Treatment with BMSC and CMMC induced a consistent and long-lasting antinociceptive effect, associated with pro-and anti-inflammatory cytokine balance modulation, as well as the reduction of structural alterations in the peripheral nerve. These data show the potential of cellular therapy in the treatment of trigeminal neuralgia .
|
|
18
|
-
LILIANE BARRETO DA SILVA
-
Potential anti-hypertensive of carvacrol uncomplexed or complexed with β-cyclodextrin in spontaneously hypertensive animals.
-
Advisor : DARIZY FLAVIA SILVA AMORIM DE VASCONCELOS
-
COMMITTEE MEMBERS :
-
DARIZY FLAVIA SILVA AMORIM DE VASCONCELOS
-
DIOGO RODRIGO DE MAGALHÃES MOREIRA
-
RODRIGO MOLINI LEAO
-
Data: Sep 14, 2018
-
-
Show Abstract
-
Introduction: The phenolic monoterpene, carvacrol, has been used as a therapeutic agent in various cardiovascular diseases, but also the physical characteristics, such as liposolubility and volatility, limit sweetness in the compound. Thus, inclusion systems of drugs such as cyclodextrins appear to allow the development of a new strategy for the use of monoterpenes in therapeutic administrations. Objective: To evaluate the antihypertensive potential of carvacrol in the free or complex form of a β- cyclodextrin (β-CD) and whether the complexation is pharmacologically favorable for the cardiovascular actions induced by this monoterpene. Methods: In vivo studies, was evaluated the actions of CARV/β-CD and carvacrol, and daily dose at a dose of 50 mg / kg, on blood pressure and heart rate, after acute oral administration. The experiments were performed in spontaneously hypertensive rats (SHR) and normotensive controls rats (wistar). In the subchronic treatment, in 21 days, was investigated antihypertensive activity of both compounds. In vitro studies, the rats were euthanized in CO2 chamber and superior mesenteric artery, spleen and aorta were removed. In mesenteric arteries were performed the vascular reactivity, in spleen and aorta were evaluated the inflammatory and anti-inflammatory cytokines levels. Results: The acute oral administration in non-anesthetized normotensive rats and SHR rats, with carvacrol and CARV/β-CD, did not produce significant changes in blood pressure levels and heart rate. In the 21-day subchronic treatment, only CARV/β-CD induced a significant reduction in blood pressure levels in SHR rats. CARV/ β-CD was able to prevent the development of hypertension in SHR rats. Additionally, the vascular reactivity assays with SHR rats, presented higher potency in relation the -1 adrenergic agonist (phenylephrine), in relation to normotensive rats. Sensitivity to sodium nitroprusside did not change in all experimental groups.The levels of the pro-inflammatory cytokine IL-1β in the spleen were significantly reduced in the CARV/ β-CD treated SHR rats, and the levels of IL-10 in the aorta increased in these animals. Conclusion: CARV/β-CD was able to prevent the development of hypertension in SHR rats. In addition, it reduced levels of the pro- inflammatory cytokine- IL-1β, in the spleen, and increase the levels of the anti-inflammatory cytokine IL-10, in the aorta of CARV/β-CD treated SHR rats.
|
|
19
|
-
JONHY HERBERT GONÇALVES EVANGELISTA
-
SCREENING OF BINDERS FROM TRIPANOTIONA REDUTASE from Tripanosoma Cruzi
-
Advisor : MARCELO SANTOS CASTILHO
-
COMMITTEE MEMBERS :
-
MARCELO SANTOS CASTILHO
-
RAQUEL GUIMARÃES BENEVIDES
-
RICARDO DAVID COUTO
-
Data: Sep 27, 2018
-
-
Show Abstract
-
Chagas disease is a neglected tropical disease caused by the protozoan Trypanosoma cruzi that affects part of the world population, and is especially acute in Brazil, where it estimated that 1.9 million people are infected by T. cruzi and approximately 21.8 million are exposed to the risk in endemic areas of the country. Although it affects more than 1.8 million people in the Americas and puts at risk of contagion another 100 million, only two drugs are used to treat it (Benznidazole and Nifurtimox), which have serious adverse effects and high toxicity, making adherence to pharmacotherapy difficult. This scenario suggests the need to study new therapeutic targets, which are essential for the survival of the parasite, such as the oxidation pathway, in which the enzyme Trypanothione reductase (TcTR) plays a fundamental role. Although TcTR is a validated target, there are still no drugs that block this enzyme. This opens up a knowledge gap for the screening of inhibitors. Among the techniques of large-scale screening (HTS) the ThermoFAD technique can be highlighted. This methodology allows the investigation of thermal stability of Flavoproteins by monitoring the fluorescence signal of an intrinsic fluorophore (FAD) as a reflection of its unfolding. In order to identify ligands with minimum structural requirements for TcTR inhibition, the thermal detachment technique (ThermoFAD) was used for the screening of compounds. For this, we present the standardization of the chromatographic purification of TcTR and investigation by ThermoFAD of the conditions (TcTR concentration, pH, additives, etc.). Single dose screening allowed the identification of compound JU298, for which concentration-response assays were also conducted, confirming its stabilizing effect on TcTR. Such data therefore suggest that the JU298 compound as a potential inhibitor for the therapeutic target under study.
|
|
20
|
-
SANDRA CRISTINA HERNANDES
-
IDENTIFICATION OF VARIABLES TO BE INVESTIGATED AS PREDICTORS OF DRUG-RELATED PROBLEMS (PRM) TO RATIONALIZE THE CLINICAL PHARMACY IN A HOSPITAL Tertiary
-
Advisor : DENIS DE MELO SOARES
-
COMMITTEE MEMBERS :
-
DENIS DE MELO SOARES
-
PABLO DE MOURA SANTOS
-
RICARDO DAVID COUTO
-
Data: Dec 11, 2018
-
-
Show Abstract
-
Introduction: This study aims to identify risk factors for the occurrence of Drug Related Problems (DRP) in patients admitted to a tertiary hospital in the city of Salvador. DRP can cause preventable damage. Methodology: This study was an observational, cross-sectional study of a retrospective nature, carried out in a large-scale, highly complex hospital with 352 beds, data from the year 2016. The prescriptions were evaluated by identifying DRP by pharmacists clinic. Results: The main DRPs observed are described in the literature and could have been avoided emphasizing the importance of prevention measures. Discussion: The presence of the pharmacist in the review of medical prescriptions plays an essential role in the activities of hospital clinical pharmacists and can collaborate to improve the quality of drug use as well as patient safety. Conclusion: Knowing the risk factors for the occurrence of DRP is of paramount importance in establishing prevention tools and strategies.
|
|
21
|
-
KELVIS TRINDADE SANTOS
-
Evaluation of the antioxidant activity of Theobroma cacao L. beans husk extracts for cosmetic applicability
-
Advisor : EUDES DA SILVA VELOZO
-
COMMITTEE MEMBERS :
-
NEILA DE PAULA PEREIRA
-
RENATA BIEGELMEYER DA SILVA RAMBO
-
SERGIO EDUARDO SOARES
-
Data: Dec 17, 2018
-
-
Show Abstract
-
The Theobroma cacao L. beans husk is a waste in the industry known to contain a large amount of polyphenols and dietary fiber. The presence of polyphenolic antioxidants present in cocoa has caused an increasing interest in the use of products derived from cocoa in cosmetic preparations. This work aimed to obtain gels with antioxidant activity through the incorporation of extracts prepared from cocoa beans husks. The study addresses two distinct phases: the first one concerning the extraction of hydroethanolic and hydroglycolic extracts, fluid and dry, of the cocoa beans husk, which also includes the verification of physico-chemical parameters (pH, density and thermogravimetry), total phenolics and antioxidant activity (DPPH method). The second phase refers to the use of these extracts in gelled cosmetic formulations that were developed with different natural polymers (xanthan gum, carboxymethylcellulose and hydroxyethylcellulose), as well as physical and chemical parameters (pH and density) and stability (organoleptic characteristics, pH, density, texture and scattering in vitro) over time and under accelerated conditions. The antioxidant activity of the formulations was also evaluated. The extracts presented good results regarding their physicochemical profile, where they had values close to pH and density, besides being safe when applied in cold or hot formulations. It presented a high antioxidant potential in the extracts, highlighting the hydroethanolic, obtaining 74.72% SRL for the fluid and close to 90% SRL for the dry, besides an IC50 of 0.24 mg/ml for
hydroethanolic and 1,15 mg/ml for the hydroglycolic. The formulations had their potential antioxidants proven, with a highlight for the gel with xanthan gum and hydroethanolic dry extract with value close to 80%. In addition, all the gels presented good performance of in vitro spreadability and texture, making them more stable when applying the extracts, preserving their values of spreadability, strength and adhesiveness. Based on the results obtained, cocoa beans husks can be used as a source of interesting raw material for cosmetology, especially in anti-aging dermotherapies.
|
|