Banca de DEFESA: ALYSSON LUIZ MENDES DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ALYSSON LUIZ MENDES DA SILVA
DATA : 17/02/2020
HORA: 09:00
LOCAL: PPGFAR
TÍTULO:

Determination of paraoxonase and arylesterase activities of PON1 enzyme on the visible spectrum range in patients with dyslipidemia: low HDL-c and hypertriglyceridemia

PALAVRAS-CHAVES:

Arylesterase; PON1; HDL-c; Hypertriglyceridemia

PÁGINAS: 55
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Disorders in lipid and lipoprotein metabolism (dyslipidemia) are common in the population and are considered risk factors for cardiovascular disease. Studies have shown enhanced cardiovascular risk in individuals with PON1 low paraoxonase activity. However, the role of the arylesterase activity of PON1, an enzyme associated with HDL, is not yet known. This cross-sectional study aimed to evaluate arylesterase/paraoxonase activity of PON1 in serum samples from normolipidemic patients and with dyslipidemia such as hypertriglyceridemia and low HDL-c. Sixty serum were evaluated and stratified in three groups. Lipid profiles were determined, apoA1 levels measured and paraoxonase/arylesterase enzymatic activity of PON1 was determined against paraoxon and phenylacetate substrates, respectively, by spectrophotometry in kinetic mode at 405nm using 96 well microplates. The assays showed a significant difference in enzyme activity between the different groups studied and a proportional reduction in PON1 enzymatic activity compared to normolipidemic patients. After the correction of enzymatic activities by apoA1 concentration, the low HDL-c group showed the largest reduction in enzymatic activity and positive linear correlation when evaluated the concentration of HDL-c and apoA1. There was also a directly proportional dependence between enzymatic activity and apoA1 levels variables under normolipidemic and low HDL-c conditions after linear regression. The results suggest that for hypertriglyceridemia conditions, there is a decreased activity to a lesser extent when compared to the condition of low HDL-c, where is observed a significant reduction in enzymatic activity of PON1.

MEMBROS DA BANCA:
Presidente - 1490663 - RICARDO DAVID COUTO
Interno - 2055299 - ANA LEONOR PARDO CAMPOS GODOY
Externo ao Programa - 1278694 - SIMONE GARCIA MACAMBIRA