Banca de DEFESA: CLARICE RIBEIRO LIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CLARICE RIBEIRO LIRA
DATA : 01/07/2020
HORA: 15:00
LOCAL: ambiente virtual
TÍTULO:

Biperiden Effects on Ethanol Self-administration in rats

PALAVRAS-CHAVES:

Ethanol; Biperiden; Relapse; Self-administration

PÁGINAS: 47
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Ethanol abuse is a major public health problem worldwide. The development of ethanol dependence occurs from plasticities in the central nervous system. It is known that the behavior of ethanol consumption can be modulated by cholinergic neurotransmission through nicotinic receptors. However, it is not well known about the interaction in between ethanol and muscarinic receptors. Biperiden is an anticholinergic drug that acts preferentially by blocking the muscarinic M1 receptor and has been promising for treatment of other substances of abuse. The aim of this work was to evaluate the involvement of biperiden in the motivational mechanisms and reinstatement of the ethanol seeking on the operant behavior animal model. For this, adult male Long Evans rats and self-administration boxes were used. Animals were trained to press a lever to receive an ethanol dose and for the tests the subjects were divided into the Control and Biperiden groups, and received 0.9% saline (1 ml / kg; i.p.) or different doses of biperiden (1.0; 2.5; 5.0 and 10.0 mg / kg; i.p.), respectively, 30 minutes before each test. The motivation to ethanol seeking was assessed using the Progressive Ratio (PR) schedule. Our results demonstrate a significant decrease in the breakpoint and in the number of reinforcements at doses 5.0 and 10.0 mg / kg (i.p.). In addition, the extended access protocol (“Binge”) for ethanol was carried out for a period of 5 hours. The results demonstrated a significant reduction in the search for ethanol in the animals treated with the doses 5.0 and 10 mg / kg (i.p.) when compared to the control. Later, the animals went through the extinction stage, where each pressure on the active lever was no longer reinforced by ethanol and it was performed in an different environment with tactile, visual, sound and circadian differences from the environment of the acquisition phase, which we call “Context B”. Twenty-four hours after the last extinction session, the context-induced reinstatement test was performed. It was observed that all doses of biperiden administered 30 minutes before the test were able to prevent the context-induced reinstatement of ethanol seeking.

MEMBROS DA BANCA:
Interno - 1881297 - DENIS DE MELO SOARES
Externo à Instituição - PAULA CRISTINA BIANCHI
Presidente - 2267989 - RODRIGO MOLINI LEAO