Banca de DEFESA: LUCIANA SANTA BARBARA BITTENCOURT

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUCIANA SANTA BARBARA BITTENCOURT
DATA : 23/08/2019
HORA: 09:00
LOCAL: Faculdade de Farmácia sala 07
TÍTULO:

FUNGEMIA IN A HIGH SCHOOL UNIVERSITY HOSPITAL: EPIDEMIOLOGICAL AND DIAGNOSTIC SURVEY OF REAL-TIME PCR CANDIDEMIA.

PALAVRAS-CHAVES:

candidemia; Real-time PCR; Fungemia Epidemiology

PÁGINAS: 104
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
SUBÁREA: Biologia e Fisiologia dos Microorganismos
ESPECIALIDADE: Micologia
RESUMO:

Invasive fungal infections have been increasingly common in recent decades, becoming one of the leading causes of morbidity and mortality in immunocompromised patients. Geographic differences in species distribution and in vitro susceptibility patterns to antifungal agents reaffirm the importance of conducting epidemiological surveillance studies of fungemia. The treatment of invasive fungal infections has been hampered by the inability to diagnose the infection at an early stage of the disease. With the need for faster strategies in the process of detection and identification of these pathogens, real-time polymerase chain reaction technology has improved the viability of the results. Therefore, the objective of this study was to make an epidemiological survey of patients with fungemia at Professor Edgard Santos University Hospital, Salvador, Bahia, from January 2018 to January 2019, diagnosing candidemia through real-time PCR assays. A total of 6204 blood cultures were processed with 9.5% positivity; of these, 91.5% positive for bacteria and 8.5% positive for fungi, being the yeast in fourth place among the most prevalent isolates. 24 individuals presented 25 isolates in fungi positive blood culture: 72% from Candida yeast, 20% Cryptococcus, 4% Histoplasma capsulatum and 4% without identification. Most patients were 58.3% male (14/24), with a mean age of 39.8 years and a median of 56.5 years. It was found that the highest prevalence was in patients with HIV positive and gastrointestinal disease, both with a percentage of 33.3%, followed by renal failure with 29.2%, neoplasia with 25% and systemic arterial hypertension with 20.8. %. Real-time PCR assays show that the molecular beacons hybridization probe of the intergenic region was fungus specific, detecting positive blood cultures for C. glabrata, C. albicans, C. krusei, C. tropicalis and C. parapsilosis, but further studies are still needed to increase the specificity of the probe, eliminating the detection of genera Aspergillus and Saccharomyces. Candida detection by real-time PCR assay was possible in blood culture samples and not in blood samples.

MEMBROS DA BANCA:
Presidente - 287717 - TANIA FRAGA BARROS
Interno - 1881297 - DENIS DE MELO SOARES
Externo à Instituição - ANA PAULA DE OLIVEIRA MENEZES - UERJ