Banca de DEFESA: SARA EMI MATOS MENDES FERREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : SARA EMI MATOS MENDES FERREIRA
DATA : 24/05/2019
HORA: 09:00
LOCAL: Faculdade de Farmácia (UFBA)
TÍTULO:

EVALUATION OF THE INVOLVEMENT OF THE FRAGILE PROTEIN OF MENTAL RETARDATION X (FMRP) IN THE DEVELOPMENT OF ETHANOL DEPENDENCE: FROM VIRTUAL SCREENING TO BEHAVIOR.

PALAVRAS-CHAVES:

Addiction; Ethanol; FMRP.

PÁGINAS: 64
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
SUBÁREA: Neuropsicofarmacologia
RESUMO:

Drug abuse is currently a global public health problem. The abusive consumption of ethanol promotes neuroplasticity and changes in balance between excitatory and inhibitory neurotransmitters, as well as altering proteins involved in gene expression, altering neuronal excitability. Chronic exposure to ethanol is capable of generating tolerance and dependence, although some aspects of dependence may occur relatively rapidly in response to acute administration, most of the changes occurring gradually occur over time in response to prolonged exposure to the drug. Chronic ethanol treatment has been shown to enhance the phosphorylation of Fragile X Mental Retardation Protein (FMRP) protein. From the increase of its activity, it was observed an increase in gene transcription and protein plasticity in glutamatergic synapses in regions important for the development of ethanol dependence. The objectives of this study are: 1. To develop a new molecule with FMRP protein antagonist activity and its modeling, using bioinformatics tools 2. To express the protein by cloning in vitro and verify the optimal conditions for its expression; 3. Evaluation of neuroadaptations through behavioral analysis of the development and expression of locomotor sensitization in the open field with male Swiss mice (30g) from the administration of ethanol (EtOH 2.2g / kg, ip) and saline (SAL 0.1ml / kg; ip) (Ethics committee Protocol 110/2016); 4. Evaluation of neuronal activation by immunohistochemistry of the regions of the Prefrontal Cortex, Nucleus Acúmbens and Amídala. Our results show the expression of FMRP in the conditions of high concentration of IPTG (1M) and high temperature in short period (37 ° C / 4hrs); In the behavioral analysis it was observed that animals treated with EtOH (chronic) showed a progressive increase in locomotor activity (initial mean of 25.41m and final mean 62.17m) during the development of locomotor sensitization and also showed greater locomotor activity than the control groups, which maintains a constant locomotion pattern, between 38 and 33 meters (average of day 1 and day 21 respectively). The chronic group also had greater locomotor activity in the expression of sensitization when compared to the other control and acute groups (mean 42.02). It was also possible to identify a tendency in the activation of the brain regions NAc and CPFm in the brains of the animals after treatment with the drug.

MEMBROS DA BANCA:
Presidente - 2267989 - RODRIGO MOLINI LEAO
Interno - 1881297 - DENIS DE MELO SOARES
Externo ao Programa - 3420773 - REJANE CONCEICAO SANTANA