Banca de DEFESA: DOURIVALDO SILVA SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DOURIVALDO SILVA SANTOS
DATA : 22/03/2019
HORA: 14:30
LOCAL: Faculdade de Farmácia
TÍTULO:

PHARMACOLOGICAL CHARACTERIZATION OF THE ANTINOCICEPTIVE PROPERTIES OF Spirulina Platensis

PALAVRAS-CHAVES:

Spirulin, pain, analgesic, anti-inflammatory, cytokines, IL-10, opioids.

PÁGINAS: 70
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
RESUMO:

Pain has a protective physiological function; however the intense or persistent pain causes suffering to the patient, requiring medication intervention. First-line drugs for pain control, opioids and nonsteroidal anti-inflammatory drugs can cause serious side effects, especially for prolonged use. Thus, adequate pain control still depends on the development of new drugs, more effective and with fewer side effects. Natural products are a promising source of bioactive substances. Spirulina platensis (SP) is a cyanobacterium that produces a wide variety of active metabolites, and has aroused the interest of the pharmaceutical and food industry. In this work, the antinociceptive properties SP were established in experimental models, investigating their possible mechanisms of action. Initially, the antinociceptive effect of SP was investigated in an inflammatory pain model. Oral administration of SP (50-400 mg / kg) produced dose-dependent inhibition of inflammatory allodynia (p <0.01) and paw edema (p <0.001) in the Freund's Complete Adjuvant (CFA) model in mice. Treatment with SP (200 mg / kg) modulated the balance of pro- and anti-inflammatory cytokines, i.e., reduced levels of hyperalgesic cytokines (TNF-α, p <0.05, IL-1β, p<0.01) and elevated those of IL-10, an anti-inflammatory cytokine (p <0.05). Treatment with SP (200 mg / kg) in IL-10 knockout mice (IL-10 / KO) did not prevent inflammatory allodynia nor the formation of paw edema induced by CFA. This result indicates that the SP-induced anti-inflammatory effects are mediated by IL-10 production. Next, we investigate whether the SP-induced antinociception present a centrally mediated component. Treatment with spirulina (100-400 mg / kg) dose-dependently increased the latency time in the tail-flick test. The antinociceptive effect of SP in the tail flick test was partially prevented by pre-treatment with naloxone (5 mg / kg), a non-selective opioid receptor antagonist (p <0.05). Thus, it is possible to propose that SP-induced antinociception has a central mediating component involving activation of opioid pathways. Mice treated with spirulina (400 mg / kg) showed no motor deficit in the rota-rod test, corroborating data from the nociceptive tests. In conclusion the present study showed that SP has relevant antinociceptive properties. Importantly, the anti-inflammatory and antinociceptive actions of this cyanobacterium appear to be independent, possibly mediated by IL-10 and opioid pathways, respectively. Considering that Spirulina platensis is already approved for human use, this study validates its clinical indication as an anti-inflammatory and allows us to propose a new as an analgesic.

MEMBROS DA BANCA:
Presidente - 1536719 - CRISTIANE FLORA VILLARREAL
Interno - 1281669 - RENATA BIEGELMEYER DA SILVA RAMBO
Externo ao Programa - 1350842 - JANICE IZABEL DRUZIAN