Banca de DEFESA: FÁBIO MOTA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : FÁBIO MOTA SILVA
DATA : 15/02/2019
HORA: 09:00
LOCAL: Faculdade de Farmácia UFBA
TÍTULO:

REAL TIME PCR WITH "MOLECULAR BEACONS" HYBRIDIZATION PROBE FOR CANDIDA SPP DETECTION IN HEMOCULTURE

PALAVRAS-CHAVES:

Candidemia; Real-time PCR; hydrolysis probe; hybridization probe; Molecular Beacons; TaqMan.

 

PÁGINAS: 58
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
SUBÁREA: Análise Toxicológica
RESUMO:

Candidemia, a bloodstream infection caused by Candida yeasts, has become a growing problem in tertiary care hospitals, in which patients suffering from the disease have high morbidity and mortality rates due to their difficulty in the early diagnosis of the disease. Thus, aiming at new methods for the detection of Candidemia, the objective of our study was to standardize real-time PCR technique with Molecular Beacons hybridization probe for the detection of Candida spp. in blood culture. To this end, a target sequence of the rDNA ITS region (ITS1-5.8S-ITS2) was chosen and from there a "Molecular Beacons" hybridization probe was developed for conducting the real-time PCR assays. The probe was standardized with DNAs from standard strains and tested with DNAs extracted from blood cultures from patients of a public hospital and a private hospital located in Salvador, Bahia. The "Molecular Beacons" hybridization probe detected the presence of yeasts of the Candida genus, including the C. albicans, C. glabrata, C. parapsilosis, C. tropicalis species in positive blood cultures of patients with candidemia, as well as yeasts of the genus Saccharomyces. The probe did not detect the presence of yeasts of the genus Cryptococcus and of the Kodamaea genus in positive blood culture of a patient with these yeasts, did not detect the presence of bacteria in positive blood cultures of patients with bacteremia, nor did it to promote positive reaction in blood cultures of patients without invasive infection. Compared with the hydrolysis probe developed by Amaral (2017), the hybridization probe "Molecular Beacons" developed with the same nucleotide sequence may demonstrate greater specificity. Therefore, the "Molecular Beacons" hybridization probe developed can be used in the real-time PCR assay for rapid detection of Candida spp. directly from blood cultures in the diagnosis of candidemia.

MEMBROS DA BANCA:
Presidente - 287717 - TANIA FRAGA BARROS
Interno - 1187203 - JOICE NEVES REIS PEDREIRA
Externo ao Programa - 2582698 - BARBARA MARIA PARANA DA SILVA SOUZA