Banca de DEFESA: RAFAELA GOMES ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : RAFAELA GOMES ALVES
DATA : 22/02/2019
HORA: 09:00
LOCAL: Faculdade de farmácia
TÍTULO:

POTENCIAL ANTINEOPLÁSICO DO ÓLEO ESSENCIAL DAS FOLHAS DE Guatteria megalophylla DIELS (ANNONACEAE)

PALAVRAS-CHAVES:

Antitumoral; Annonaceae; Cytotoxicity; Essential oil.

PÁGINAS: 69
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

 

Introduction: Essential oils obtained from several plant species have been considered as potential sources of biologically active substances, presenting several therapeutic properties. The Guatteria megalophylla Diels (Annonaceae) plant is a tree native to the Brazilian and Peruvian Amazon rainforest. Its cytotoxic potential had not yet been described in literature; however, within its family there have already been reports of several species with pharmacological potential, including antitumoral activity. Objective: To evaluate the antineoplastic potential of the essential oil from G. megalophylla leaves. Methods: The chemical composition of the essential oil was determined by gas chromatography with flame ionization and mass detectors. The cytotoxic activity of the essential oil was tested in different human cancer cell lines, HL-60 (acute promyelocytic leukemia), MCF-7 (breast carcinoma), CAL27 (tongue squamous cell carcinoma), HSC-3 (tongue carcinoma), HepG2 (hepatocellular carcinoma) and HCT116 (colon carcinoma), and non-cancer cell line, MRC-5 (human lung fibroblast) by the alamar blue method after 72h incubation. Subsequently, HL60 cells were incubated for 24 and 48h with the essential oil (10, 20 and 40 μg/mL) and the number of viable cells was determined by the trypan blue exclusion assay, and cell cycle analysis, mitochondrial transmembrane potential, as well as quantification of reactive oxygen species (ROS) were evaluated by flow cytometry. Results: The essential oil was the major constituent of spathulenol and showed IC50 values for cancer cells ranging from 12.5 to 39.5 μg/mL for HL-60 and CAL27, respectively. The IC50 value for the MRC-5 non-cancer cells was 29.8 μg/mL. The cytotoxic effect of the essential oil was confirmed by the tripan blue exclusion assay, where it showed a reduction in the number of viable cells. It was also observed that the essential oil induces internucleosomal DNA fragmentation after 24 and 48h and induces depolarization of the mitochondrial transmembrane potential, but did not induce ROS production. In addition, the essential oil inhibited the in vivo development of HL-60 cells inoculated in C.B-17 SCID mice. Tumor mass inhibition rates were 16.63% at 50 mg/kg concentration and 48.79% at 100 mg/kg concentration. Conclusion: The essential oil of G. megalophylla leaves may be a promising phytotherapeutic substance for the treatment of several types of cancers since it exhibits cytotoxicity for different carcinogenic cell lines, in addition to presenting antitumor activity in vivo.

MEMBROS DA BANCA:
Interno - 1536719 - CRISTIANE FLORA VILLARREAL
Presidente - 846.758.293-68 - DANIEL PEREIRA BEZERRA - UFC
Externo ao Programa - 2436890 - JUNIA RAQUEL DUTRA FERREIRA