Banca de DEFESA: MATHEUS ANTÔNIO DA HORA BORGES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MATHEUS ANTÔNIO DA HORA BORGES
DATE: 07/12/2023
TIME: 15:00
LOCAL: Online no RNP
TITLE:

BIOANALYTIC VALIDATION, PHARMACOKINETIC ANALYSIS AND PREPARATION OF AN α BISABOLOL NANOEMULSION

KEY WORDS:

α-bisabolol; Bioanalytical validation; Pre-clinical pharmacokinetics; nanotechnology.

PAGES: 63
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Natural products are the greatest basis for the discovery and development of new drugs. Chamomile is an herb commonly used in the preparation of teas by different cultures and has several reported therapeutic effects. α-bisabolol (α-BIS), its main constituent, has been the target of several pharmacological studies due to its ability to act on different body systems, from inflammatory processes and pain modulation to the fight against microorganisms. Despite knowledge about their effects, the behavior of drugs in the body has not yet been elucidated, as there are no pharmacokinetic studies on them. Knowing pharmacokinetics makes it easier to understand the pharmacodynamic mechanisms in which they participate. For pharmacokinetic analysis, a bioanalytical method is necessary so that it is possible to quantify plasma concentrations of the compound after administration. Therefore, the objective of this work was to develop and validate a bioanalytical method for the determination of α-bisabolol in plasma using High-Performance Liquid Chromatography (HPLC) and subsequently evaluate its pharmacokinetics. To achieve this, the method was developed in accordance with the requirements recommended by the Food and Drug Administration (FDA) and the Agência Nacional de Vigilância Sanitária (ANVISA). Chromatographic conditions consisted of an isocratic flow of 1 ml/min of acetonitrile and ultrapure water (80:20). The developed method proved to be precise, accurate, sensitive, and selective for quantifying the compound up to one month after freezing. To demonstrate the method’s applicability, a pharmacokinetic analysis of α-BIS was performed after intravenous administration of the compound in Wistar rats. After this administration, pharmacokinetic parameters such as volume of distribution, clearance, and half-life. With this validated method and pharmacokinetic analysis carried out, it becomes possible to develop new in vivo studies that aim to quantify α-bisabolol in biological matrices, in addition to assisting in new pharmacodynamic studies, since, for the first time to date, the pharmacokinetics of the compound were described. 

COMMITTEE MEMBERS:
Presidente - 1012812 - FRANCINE JOHANSSON AZEREDO
Interna - 1528081 - DARIZY FLAVIA SILVA AMORIM DE VASCONCELOS
Externa à Instituição - SANDRA ELISA HAAS - Unipampa