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MÓNICA MADRIGAL VALVERDE
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Uretral Sperm criopreservarion on Domestic and Wild Feline, sperm parameters and corporal and testicular biometry correlation.
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Leader : ANTONIO DE LISBOA RIBEIRO FILHO
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MEMBRES DE LA BANQUE :
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ANTONIO DE LISBOA RIBEIRO FILHO
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MARIA CONSUELO CARIBE AYRES
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VIVIAN FERNANDA BARBOSA
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ANA KARINA DA SILVA CAVALCANTE
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Data: 4 févr. 2019
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Afficher le Résumé
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The information obtained on the biology and reproductive biotechnology of the domestic cat allows a correlation and application in feline wild species. The study of the physical characteristics and their association with reproductive parameters allows to increase the selection criteria of individuals for reproduction of endangered species. Semen cryopreservation is a fundamental component for assisted reproduction programs in wild felids. The objective of this study was to evaluate the association between the biometric measurements and the before and after freezing morphokinetic characteristics of feline domestic and wild species sperm , obtained by uretral cateterization using two different drugs and processed with three seminal extenders . For this study, 13 feline males (Felis catus), selected and separated into groups, according to the drug tested (Experiment 1). In the first experimental group (GD1), the combination of medetomidine hydrochloride (0.1 mg / kg) and ketamine (5 mg / kg) was used and in the second group (GD2) the combination of dexmedetomidine hydrochloride (1 mg / kg ) and ketamine (5 mg / kg). After the required anesthetic level was reached, the animals were submitted to probing with urethral catheter, and the semen was collected and transported to the laboratory for analysis. We evaluated the semen quality characteristics of fresh and frozen-thawed sperm with conventional microscopy: volume, total motility and progressive motility, percentage of spermatozoa with membrane functional integrity (osmotic shock) and membrane structural integrity (eosin stain), sperm concentration and sperm morfology. There was no difference in sperm parameters (P> 0.05) into groups GD1 and GD2. The samples were cryopreserved in three groups of cryoprotectants (Experiment 2), GC1 with 6% glycerol ( GLI ), GC2 with 3% dimethylacetamide ( DMA ) GC3 with 3% dimethylformamide ( DMF ). For frozen-thawed semen, kinetic parameters in computerized analysis equipment (CASA) and fluorescence in flow cytometry were also evaluated, when there were also no differences (P> 0.05) for the different parameters evaluated between the groups studied. The study of spermatic subpopulations identified four subpopulations with independent behaviors defined between them. After the semen measurements and collection, the animals were submitted to orchidectomy. In the animals, corporal and testicular measurements were performed: head length, head circumference, head +body length, thoracic diameters, tail length and total length, width, length, thickness of each testicle, and body weight (Experiment 3). In addition, the testes of the animals were measured with ultrasonography. Post-orchiectomy testicles were also measured and weighed. The estimated testicular parameters were testicular area, testicular volume, testicular weight and gonadosomatic index. We compared the methods of testicular measurements and estimated linear Pearson correlations between body biometry, testicular and sperm parameters. There were no differences between the measurements obtained with the two methods of measuring the testicles (P> 0.05). Positive correlations (P <0.05) were observed between total length and testicular parameters, as well as between sperm concentration and testicular parameters. For the study with the wild felids, a Leopardus wiedii, a Puma yaguaroundi and an Panthera ounce. The animals were submitted to chemical ejaculation with the combination of medetomidine hydrochloride (0.1 mg / kg) and ketamine (5 mg / kg) IM. Seminal samples collected by urethral catheterization were frizing with the same cryoprotectants and concentrations of Experiment 2. The evaluations of the in fresh and post-thaw semen were the same as described for Experiment 1 and 2, which comparison between the different groups resulted in Experiment 4 For the latter study, no differences were found for the extender used (P> 0.05). It was concluded that there are no differences in sperm quality in fresh and post-thawing. However, testicular biometry exerts an important influence on the sperm concentration of the breeders, being an adequate parameter to be considered in felid breeding.
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2
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MÓNICA MADRIGAL VALVERDE
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Urethral Sperm cryopreservation on Domestic and Wild Feline.
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Leader : ANTONIO DE LISBOA RIBEIRO FILHO
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MEMBRES DE LA BANQUE :
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ANTONIO DE LISBOA RIBEIRO FILHO
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MARIA CONSUELO CARIBE AYRES
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VIVIAN FERNANDA BARBOSA
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ANA KARINA DA SILVA CAVALCANTE
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Data: 4 févr. 2019
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Afficher le Résumé
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Information obtained on the biology and reproductive biotechnology of the domestic cat allows a correlation and application in feline wild species. The study of the physical characteristics and their association with reproductive parameters allows increasing the selection criteria of individuals for reproduction of endangered species. Semen cryopreservation is a fundamental component for assisted reproduction programs in wild felids. Objective of this study was to evaluate the association between the biometric measurements and the previous and post freezing morphokinetic characteristics of feline domestic and wild species sperm, obtained by urethral catheterization using two different drugs and processed with three seminal extenders. For this study, 13 feline males (Felis catus), selected and separated into groups, according to the drug tested (Experiment 1). In the first experimental group (GD1), the combination of medetomidine hydrochloride (0.1mg/kg) and ketamine (5mg/kg) was used and in the second group (GD2) the combination of dexmedetomidine hydrochloride (0.025mg/kg) and ketamine (5mg/kg). After the required anesthetic level was reached, the animals were submitted to probing with urethral catheter, and the semen was collected and transported to the laboratory for analysis. We evaluated the semen quality characteristics of fresh and frozen-thawed sperm with conventional microscopy: volume, total motility and progressive motility, percentage of spermatozoa with membrane functional integrity (osmotic shock) and membrane structural integrity (eosin stain), sperm concentration and sperm morphology. There was no difference in sperm parameters (P> 0.05) into groups GD1 and GD2. The samples were cryopreserved in three groups of cryoprotectants (Experiment 2), GC1 with 6% glycerol (GLI), GC2 with 3% dimethylacetamide (DMA) GC3 with 3% dimethylformamide (DMF). For frozen-thawed semen, kinetic parameters in computerized analysis equipment (CASA) and fluorescence in flow cytometry were also evaluated, when there were also no differences (P> 0.05) for the parameters evaluated between the groups studied. The study of spermatic subpopulations identified four subpopulations with independent behaviors defined between them. After the semen collection and obtainment of its parameters, the animals were submitted to orchidectomy. In the animals, corporal and testicular measurements were performed: head length, head circumference, head +body length, thoracic diameters, tail length and total length, width, length, thickness of each testicle, and body weight (Experiment 3). In addition, the testicles of the animals were measured with ultrasonography. Post-orchiectomy testicles were also measured and weighed. The estimated testicular parameters were testicular area, testicular volume, testicular weight and gonadosomatic index. We compared the methods of testicular measurements and estimated linear Pearson correlations between body biometry, testicular and sperm parameters. There were no differences between the measurements obtained with the two measurement methods of the testicles (P> 0.05). Positive correlations xiii (P <0.05) were observed between total length and testicular parameters, as well as between sperm concentration and testicular parameters. For the study with the wild felids, a Leopardus wiedii, a Puma yagouaroundi and a Panthera onca ounce were included. The animals were submitted to chemical ejaculation with the combination of medetomidine hydrochloride (0.1mg/kg) and ketamine (5mg/kg) IM. Seminal samples were collected by urethral catheterization were frozen with the same cryoprotectants and concentrations of Experiment 2. For the latter study, no differences were found for the extender used (P> 0.05). It was concluded that there are no differences in sperm quality in fresh and post-thawing. However, testicular biometry exerts an important influence on the sperm concentration of the breeders, being an adequate parameter to be considered in felid breeding.
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3
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MÓNICA MADRIGAL VALVERDE
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Urethral Sperm cryopreservation on Domestic and Wild Feline.
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Leader : ANTONIO DE LISBOA RIBEIRO FILHO
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MEMBRES DE LA BANQUE :
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ANA KARINA DA SILVA CAVALCANTE
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ANTONIO DE LISBOA RIBEIRO FILHO
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MARIA CONSUELO CARIBE AYRES
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VIVIAN FERNANDA BARBOSA
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Data: 4 févr. 2019
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Afficher le Résumé
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Information obtained on the biology and reproductive biotechnology of the domestic cat allows a correlation and application in feline wild species. The study of the physical characteristics and their association with reproductive parameters allows increasing the selection criteria of individuals for reproduction of endangered species. Semen cryopreservation is a fundamental component for assisted reproduction programs in wild felids. Objective of this study was to evaluate the association between the biometric measurements and the previous and post freezing morphokinetic characteristics of feline domestic and wild species sperm, obtained by urethral catheterization using two different drugs and processed with three seminal extenders. For this study, 13 feline males (Felis catus), selected and separated into groups, according to the drug tested (Experiment 1). In the first experimental group (GD1), the combination of medetomidine hydrochloride (0.1mg/kg) and ketamine (5mg/kg) was used and in the second group (GD2) the combination of dexmedetomidine hydrochloride (0.025mg/kg) and ketamine (5mg/kg). After the required anesthetic level was reached, the animals were submitted to probing with urethral catheter, and the semen was collected and transported to the laboratory for analysis. We evaluated the semen quality characteristics of fresh and frozen-thawed sperm with conventional microscopy: volume, total motility and progressive motility, percentage of spermatozoa with membrane functional integrity (osmotic shock) and membrane structural integrity (eosin stain), sperm concentration and sperm morphology. There was no difference in sperm parameters (P> 0.05) into groups GD1 and GD2. The samples were cryopreserved in three groups of cryoprotectants (Experiment 2), GC1 with 6% glycerol (GLI), GC2 with 3% dimethylacetamide (DMA) GC3 with 3% dimethylformamide (DMF). For frozen-thawed semen, kinetic parameters in computerized analysis equipment (CASA) and fluorescence in flow cytometry were also evaluated, when there were also no differences (P> 0.05) for the parameters evaluated between the groups studied. The study of spermatic subpopulations identified four subpopulations with independent behaviors defined between them. After the semen collection and obtainment of its parameters, the animals were submitted to orchidectomy. In the animals, corporal and testicular measurements were performed: head length, head circumference, head +body length, thoracic diameters, tail length and total length, width, length, thickness of each testicle, and body weight (Experiment 3). In addition, the testicles of the animals were measured with ultrasonography. Post-orchiectomy testicles were also measured and weighed. The estimated testicular parameters were testicular area, testicular volume, testicular weight and gonadosomatic index. We compared the methods of testicular measurements and estimated linear Pearson correlations between body biometry, testicular and sperm parameters. There were no differences between the measurements obtained with the two measurement methods of the testicles (P> 0.05). Positive correlations xiii (P <0.05) were observed between total length and testicular parameters, as well as between sperm concentration and testicular parameters. For the study with the wild felids, a Leopardus wiedii, a Puma yagouaroundi and a Panthera onca ounce were included. The animals were submitted to chemical ejaculation with the combination of medetomidine hydrochloride (0.1mg/kg) and ketamine (5mg/kg) IM. Seminal samples were collected by urethral catheterization were frozen with the same cryoprotectants and concentrations of Experiment 2. For the latter study, no differences were found for the extender used (P> 0.05). It was concluded that there are no differences in sperm quality in fresh and post-thawing. However, testicular biometry exerts an important influence on the sperm concentration of the breeders, being an adequate parameter to be considered in felid breeding.
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4
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THANIELLE NOVAES FONTES
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Copper deficiency in goats and sheep of the state of Bahia.
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Leader : TIAGO DA CUNHA PEIXOTO
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MEMBRES DE LA BANQUE :
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ALESSANDRA ESTRELA DA SILVA LIMA
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MOISES DIAS FREITAS
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TIAGO DA CUNHA PEIXOTO
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VITOR SANTIAGO DE CARVALHO
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Data: 4 févr. 2019
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Afficher le Résumé
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The objective of this study was to describe for the first time the clinical-epidemiological, laboratory and pathological aspects of different forms of enzootic ataxia in goats and lambs in the state of Bahia, as well as to propose an emergency oral treatment with copper sulfate in an individualized way for neonates. Three outbreaks of enzootic ataxia were studied. The history, clinical signs and epidemiological data were obtained from the owners and checked during the visits. The first outbreak (PS) occurred in 2013 in a property located in the municipality of Itaberaba and affected a herd composed of 90 goats and 130 sheep. The second outbreak (SS) occurred in 2014 in the municipality of Santa Luz and affected a herd of 90 goats and 110 sheep. The third outbreak (TS) occurred in 2018, in a property located in the same municipality of SS, being the herd constituted by 80 sheep. Samples of blood were collected from all goats and sheep treated for serum copper dosage. Nine animals (five goats and four sheep) that presented a more severe clinical picture and unfavorable prognosis were necropsied for diagnostic confirmation. During the necropsy of the PS, SS and TS animals liver samples were collected for hepatic copper dosing. At the site where SS occurred, samples of soil and the main forages where the animals were kept were collected in order to determine copper, iron, molybdenum, sulfur and zinc contents. The clinical and histopathological findings of the three outbreaks studied were classics of enzootic ataxia. In the outbreaks studied, the prevalence of enzootic ataxia was higher in kids (68) than in lambs (46) and presented high case fatality rates of 84.3% and 92.2%, respectively. The mean values of serum copper (PS, SS and TS) and hepatic (PS and SS) of goats (serum - 0.05mg / kg, hepatic - 2.88mg / kg) and sheep (serum - 0.015mg / kg) is much lower than the reference values, being at least 12 times lower in goats and 40 times in sheep. The levels of iron and sulfur were high in the fodder of the property where SS occurred, already in the soil, iron levels were high and copper levels were reduced. The treatment tested in neonates and lambs with two doses of copper sulphate solution orally at a dose of 20 mg / kg in the first and second week of life was effective in preventing enzootic ataxia in neonates and to be used for emergency disease control. The occurrence of enzootic ataxia (congenital and late form) in goats and sheep was first reported in Bahia. It is also worth noting that this disease caused by copper deficiency has caused great damage to the breeders of small ruminants, mainly due to the high mortality of the animals affected.
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5
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CLEUSA MARIA CARNEIRO OLIVEIRA
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Evaluation of the immunofuorescent antibody technique to identify dogs exposure to Sarcocystis neurona in rural areas of the city of Ilhéus, Bahia
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Leader : LUIS FERNANDO PITA GONDIM
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MEMBRES DE LA BANQUE :
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LUIS FERNANDO PITA GONDIM
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CARLOS ROBERTO FRANKE
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ALEXANDRE MORAES PINHEIRO
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THIAGO CAMPANHARO BAHIENSE
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Data: 15 févr. 2019
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Afficher le Résumé
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Sarcocystis neurona is a coccidian parasite that is the major cause of equine protozoal myeloencephalitis. Infection by S. neurona and induction of neurologic disease in dogs have been reported in North America, where the dog is considered an aberrant or accidental host of the protozoa. The dog is also definitive host of Sarcocystis cruzi and other species. The aim of this study was to test dog sera from rural areas of the city of Ilhéus, Bahia, to S. neurona and to evaluate potential serologic cross-reactivity to Sarcocystis cruzi by the immunofluorescent antibody technique (IFAT). Sera from 365 dogs were tested to S. neurona using merozoites produced in cell culture, and to S. cruzi, employing bradyzoites extracted from bovine myocardium confrimed by PCR and sequencing. The results obtained were evaluated with the following data about the dogs: age, gender, breed, residence, contact with horses, stockyard and wild animals, inhabiting close to forest, hunting habits, neurological disease and anti-parasite treatment, in order to gather better epidemiological information. As results, the frequency of dogs exposed to S. neurona was 3.83% and to S. cruzi was 6.57%., being that four samples (1.09%) were simultaneously positive for both antigens. Statistical analysis did not indicate association of antibodies to S. neurona and any of the analyzed variables. In conclusion, sera from dogs were reactive to S. neurona and S. cruzi antigens in the studied region. Serologic cross-reactivity probably doesn’t occurs for the species S. neurona and S. cruzi in dogs, or the animals were exposed to infectivity forms from both parasites or to genetically-related Sarcocystis species.
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6
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LUIZ DI PAOLO MAGGITTI JUNIOR
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Follicular dynamics, luteal function and moment of ovulation in FTAI protocols in buffaloes (Bubalus bubalis).
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Leader : ANTONIO DE LISBOA RIBEIRO FILHO
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MEMBRES DE LA BANQUE :
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ANTONIO DE LISBOA RIBEIRO FILHO
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JOSE VASCONCELOS LIMA OLIVEIRA
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MARCUS VINICIUS GALVAO LOIOLA
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MAX VITÓRIA RESENDE
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Data: 19 févr. 2019
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Afficher le Résumé
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In order to evaluate the effects of eCG, BE and GnRH on the follicular dynamics and luteal characteristics of lactating buffaloes (Bubalus bubalis), 40 females were submitted to an FTAI protocol. At day 0 all animals received an intravaginal P4 device, associated with 2mg of BE and 0.5mg of sodium cloprostenol. In D9, the implant was removed and 0.5mg of sodium cloprostenol was administered in all animals and 400UI of eCG in half of the animals. In the D10 (24 hours after implant removal) half of the females treated with eCG and half of the females not treated with eCG received 1,0mg of BE. In D11 the other half of the eCG treated females and the untreated females received 10μg of a GnRH analogue (buserelin acetate). In this way, the four experimental groups were constituted: C/eCG+GnRH (n= 10), S/eCG+GnRH (n= 10), C/eCG+BE (n= 10) and S/eCG+BE (n= 10). With the use of B-mode and power-Doppler ultrasonography, DFol (D9, D10 and D11), DFOV, TXFol, VFOV, %VFOV, MOV and TxOV were evaluated in follicular dynamics, in addition to DCL D18, VCL and dosages of P4 (D18) for the luteal function. Statistical analyzes were performed using the Tukey test, Kruskal-Wallis test and repeated measures ANOVA with a significance level of 5%. Significant statistical differences were found for DCL D18 with 1,78±0,12cm in the group (C/eCG+BE) and 1,62±0,11cm in the group (S/eCG+BE), in addition, in the P4 between the treatments C/eCG+GnRH (4,09±0,94 ng/mL) and S/eGC+GnRH (2,80±0,70 ng/mL). Regarding the variables evaluated by the power-Doppler mode, no statistical difference was found between the experimental groups. It was concluded that the use of BE or GnRH associated to eCG allowed efficient synchronization and induction of ovulation in lactating buffaloes submitted to an FITAI protocol, and should also take into account the cost-benefit factor favorable to the use of BE.
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7
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ARIANE DE JESUS LACERDA
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Profile ferning test in wild animals
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Leader : ARIANNE PONTES ORIA
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MEMBRES DE LA BANQUE :
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ARIANNE PONTES ORIA
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RICARDO WAGNER DIAS PORTELA
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RENATA MARIA MONÇÃO DA SILVA
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ASTRIA DIAS FERRÃO GONZALES
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Data: 19 févr. 2019
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Afficher le Résumé
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The tear ferning test is a qualitative – quantitative method commonly used for humans to assess the ocular surface. It has been already described for healthy patients and those with different diseases. However, scarce are the descriptions for this test in some wild species, so the aim of the present study was to evaluate tear ferning of reptiles and birds, describing the differences observed. Forty healthy, adult birds (80 eyes) and 25 reptiles (50 eyes) were used - 10 Ara ararauna, Amazona aestiva, Tyto alba, Rupornis magnirostris, Chelonoidis carbonaria, Caiman latirostris, and 5 Caretta caretta. Sampled tears were collected using absorbent paper millimeter strips and deposited in a glass slide to dry in room temperature and humidity. Crystallization patterns were observed in polarized light microscope and classified according Rolando and Masmali scales. Tear ferning patterns using Rolando scale ranged from I to II and from 0 to 2 using Masmali scale for birds; and from II to IV (Rolando) and from 2 to 4 (Masmali) for reptiles. The evaluators have given higher grades for some species; however, this condition could not be attributed to disease state, but to differences in tear composition. There were no similar crystallization patterns among phylogenetically related species or exposed to the same habitat. Tear ferning test still is an insipient tool to assess tear film quality in veterinary medicine and cannot be considered as a routine methodology.
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8
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MAURICIO ALCANTARA KALIL
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Brazilian green propolis as a therapeutic agent in the post-surgical treatment of caseous lymphadenitis.
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Leader : RICARDO WAGNER DIAS PORTELA
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MEMBRES DE LA BANQUE :
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MARCELO ANDRES UMSZA GUEZ
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MELISSA HANZEN PINNA VALENTIM
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NUBIA SEYFFERT
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RICARDO WAGNER DIAS PORTELA
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Data: 20 févr. 2019
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Afficher le Résumé
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The treatment of caseous lymphadenitis with antibiotics is not effective, requiring the surgical excision of the lesions. The 10% iodine solution is currently the choice for the post-surgical treatment, but may present histotoxicity. Considering that green propolis has proven to have antibacterial and wound healing properties, this study aimed to evaluate the use of a green propolis-based ointment as a post-surgical therapeutic option in the treatment of caseous lymphadenitis. Thirty-eight sheep were submitted to surgery for the excision of caseous lesions, and then divided into two groups, (1) iodine treatment, and (2) treatment with green propolis ointment. Clinical data of the animals, the size of the scar area and the presence of moisture and secretion in the surgical wound, as well as the humoral immune response against the bacterium, were analyzed. The green propolis treated group completely healed the surgical wound one week before the iodine treated group, with fewer cases of wound secretion, although not statistically different from the iodine treated group. No clinical signs were found to indicate toxicity or other side effects of propolis use, associated with faster and more organized hair recovery. It is concluded that the green propolis ointment can be used in the post-surgical treatment of caseous lymphadenitis in small ruminants, due to its significant effect on healing and inhibition of wound contamination, with a better esthetic effect after the final recovery of the animal.
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9
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LEVI OLIVEIRA DOS SANTOS
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Mechanical evaluation of bovine cortical bone fractions added to polymethylmethacrylate.
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Leader : JOAO MOREIRA DA COSTA NETO
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MEMBRES DE LA BANQUE :
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EMANOEL FERREIRA MARTINS FILHO
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JOAO MOREIRA DA COSTA NETO
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THEREZA CRISTINA BORIO DOS SANTOS CALMON DE BITTENCOURT
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VIVIAN FERNANDA BARBOSA
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Data: 21 févr. 2019
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Afficher le Résumé
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The aim of this study was to compare the maximum polymerization temperature and the mechanical strength of composites containing different concentrations of bovine cortical bone (OCB) added to polymethylmethacrylate (PMMA), by the axial compression test. It was used chemical activated acrylic resin and OCB powder obtained from the diaphysis of fresh bovine tibiae, which were subjected to mechanical cleaning, washing, degreasing, bleaching, drying, fragmentation, particle size standardization in 1 mm and autoclave sterilization. The experimental groups were divided into a control group (CG) containing only of PMMA, group 1 (G1) containing 90% PMMA and 10% OCB, group 2 (G2) containing 80% PMMA and 20% OCB, group 3 (G3) containing 70% PMMA and 30% OCB and group 4 (G4) containing 60% PMMA and 40% OCB. Five specimens were prepared for each experimental group by manual mixing method of the components, using stainless steel bowl and spatula, and a cylindrical polypropylene mold. The measurement of the polymerization temperature was get from handmade spherical structure by using an Infrared Laser Digital Thermometer. The mechanical axial compression test was performed in a manual electric press. The groups containing OCB powder presented a significant reduction in the average polymerization temperatures and, except for G4, did not differ from GC in the axial compressive strength. Therefore, it was concluded groups G1, G2 and G3 presented lower average polymerization temperature values and similar axial compressive strength values compared to GC.
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10
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MUCIO FERNANDO FERRARO DE MENDONCA
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Spontaneous intoxication by Prosopis juliflora in cattle and goats in the State of Bahia
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Leader : TIAGO DA CUNHA PEIXOTO
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MEMBRES DE LA BANQUE :
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TIAGO DA CUNHA PEIXOTO
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MONICA MATTOS DOS SANTOS
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JOSELITO NUNES COSTA
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LUCIANO DA ANUNCIAÇÃO PIMENTEL
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Data: 22 févr. 2019
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Afficher le Résumé
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The objective of this study was to describe the occurrence of outbreaks of spontaneous intoxication due to the consumption of Prosopis juliflora pods in cattle and goats in the State of Bahia. The history of ingestion of "mesquite beans" pods along with classic clinical signs of the "jaw and tonque trouble" and epidemiological data of the four outbreaks were obtained from technical visits, with the assistance of Veterinary Doctors of the CDP / UFBA and UFRB responsible for the visits and by rural staff. The first outbreak (PS) occurred in May 2011 at a property located in the municipality of Juazeiro and affected a herd of 150 goats. The Second Surge (SS) occurred in February 2015 in the municipality of Iaçu and reached a herd composed of 20 goats. The third outbreak (TS) occurred in July and September 2014, in a rural property located in the municipality of Tucano and affected a herd of 30 cattle. The Outbreak Room (QS) happened in August of 2016, in a property consisting of a herd of 110 cattle. Of the seven animals that presented a severe clinical condition and poor prognosis, six were euthanized and necropsied for diagnostic confirmation, four goats (C1-PS, C2-PS, C3-SS and C4-SS) and two bovines (B1-TS and B2 -TS), a bovine (B3-QS) was not allowed its euthanasia. During necropsy samples of the CNS and masseter muscle fixed in 10% formalin were collected. The clinical findings (PS, SS, TS and QS) and histopathological findings (PS, SS and TS) verified in the outbreaks reported here were compatible with algaroba intoxications. Clinically we observed mainly progressive weight loss, atrophy of chewing muscles, pendulous mandible, tongue protrusion, difficulty in seizing and chewing food, tilting of the "jaw and tonque trouble" head during chewing or rumination and excessive salivation. In the laboratory tests of the animals evaluated (C3-SS, C4-SS and B3-QS) anemia and hypoproteinemia were observed. At necropsy he observed cachexia, atrophy, paleness of the masseter muscle and recognizable fragments of "mesquite beans" pods. Histologically, it observed degeneration of the motor nucleus of the trigeminal nerve and atrophy by denervation of the masseter muscle with replacement by fibrous tissue. Control measures and prophylaxis of intoxication and management with "algaroba", it is discussed the need to develop research to determine the economic viability and sustainability of the algaroba exploiting its forage potential (animal feeding and commercialization of pods) and timber (production of firewood, coal, cuttings and mourners). P. juliflora intoxication with necroscopic and histopathological record in cattle and goats was reported for the first time in BA. Showing to be an important disease for both species in the State.
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11
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RACHEL FERREIRA
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Cystoisospora felis in cellular culture and production of monozoic cysts in vitro.
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Leader : LUIS FERNANDO PITA GONDIM
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MEMBRES DE LA BANQUE :
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LUIS FERNANDO PITA GONDIM
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CARLOS ROBERTO FRANKE
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WENDELL MARCELO DE SOUZA PERINOTTO
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LEANE SOUZA QUEIROZ GONDIM
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Data: 22 févr. 2019
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Afficher le Résumé
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Cystoisospora felis (synonym: Isospora felis) is a coccidian shed in the feces of domestic cats. The infection in cats occur upon ingestion of sporulated oocysts or ingestion of intermediate hosts infected with the parasite. These protozoa, after infecting intermediate hosts, such as mice, induce the formation of extraintestinal monozoic cysts. The aim of this study was to characterize the development of C. felis in cell culture. The parasite oocysts, excreted in the feces of a naturally infected cats, were induced to sporulate in 2% potassium dichromate and stored at 4°C until their utilization. For excystation of sporozoites and subsequent infection of cell culture, a decontamination method was applied to the fecal solution containing oocysts, followed by disruption of oocysts using glass beads. All batches of oocysts used in the current study, despite their storing time (all lower than a year), were infective to cell cultures. Extracellular sporozoites observed on cell monolayers immediately post-inoculation (PI) were in motion. When observed 24 hours PI, sporozoites were intracellular, with no motion under optical microscopy, and possessing enlarged sizes. Three days PI, parasitophorous vacuoles (PV) compatible with monozoic cysts presented variations in size and a single zoite in each PV. Cells infected with C. felis were maintained by replacing the culture medium every 48 hours, until five days PI, as at this time frame the rapid growth of VERO cells compromised the visualization of the monozoic cysts. The in vitro growth of C. felis monozoic cysts, so far never reported in literature, may favor numerous studies in the future, including tests of sensitivity and resistance to anticoccidial drugs. In addition, it may serve as a model to studies on Cystoisospora belli, the causative agent of human coccidiosis, as well as studies on related coccidia, such as Cystoisospora suis, Toxoplasma gondii and Neospora caninum.
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12
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MARCIO DE ALMEIDA COUTO ANDRADE
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Geographic informations system applied in the planning of the animal anti-rabies vaccination campaign
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Leader : CARLOS ROBERTO FRANKE
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MEMBRES DE LA BANQUE :
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ARISTEU VIEIRA DA SILVA
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CARLOS ROBERTO FRANKE
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MANUELA DA SILVA SOLCA
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NADIA ROSSI DE ALMEIDA
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Data: 22 févr. 2019
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Afficher le Résumé
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The rabies is one of the zoonoses with highest impact in public and animal health due to the high letality and costs necessary to human prophylaxis. Nowdays the reintroduction risk of the rabies vírus in urban centers arise with the presence of infected bats whom can transmit the vírus to domestic animals and humans. This current epidemiologic scenario of the rabies has been observed in Salvador with the record of many cases of infected bats during the last years, wich results in the need of avaliation the current methodology applied in campaigns of animal anti-rabies vaccination optimizing the vacination coverage of canine and feline population in municipality. The presente study aims to generate spacialized informations about the best distribution of the vaccination posts, possible uncovered and risk áreas in addition review the vaccination coverage of dogs and cats in the year of 2017 in Salvador/BA. Until the current stage of this study three maps of Kernel density were produced about the populacional concentration and the localization of the vaccination posts in seven sanitary districts ofSalvador referring to anti-rabies campaign of 2017.Until the end of the project we will finish the analysis in thw whole municipality and we will inlcude ambiental and socieconomic factors with the aim to test possible indicators to the improvement of the control of the canine and feline rabies in the municipality.
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13
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WALERIA BORGES DA SILVA
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Antigenic comparison between Sarcocystis neurona and Sarcocystis falcatula-like isolated in vitro in Bahia.
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Leader : LUIS FERNANDO PITA GONDIM
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MEMBRES DE LA BANQUE :
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LUIS FERNANDO PITA GONDIM
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CLARISSE SIMOES COELHO
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FLAVIANE ALVES DE PINHO
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WENDELL MARCELO DE SOUZA PERINOTTO
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Data: 22 févr. 2019
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Afficher le Résumé
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Sarcocystis neurona is a coccidian parasite, considered the causative agente of equine protozoal mieloencephalitis (EPM). Opossums of the genus Didelphis are known as definitive hosts of S. neurona. Four species of opossums (Didelphis sp.) are found in Brazil. The aim of this study was to compare a national isolate of Sarcocystis sp. with a strain of S. neurona for potential use in serological tests. 217 equine serum samples were tested by indirect Immunofluorescence technique (IFAT) with merozoites produced in the cell culture of the two parasites. Preliminary results at IFAT revealed that frequency of exposing animals to S. neurona was 7.83% and Sarcocystis sp. 8.76%. Eight seropositive animals by IFAT against S. neurona were tested by Western blot with non-reduced S. neurona antigen. Proteins with molecular weight of 18 kDA was identified. Preliminary results, with positive reaction in both strains, allow two possible conclusions, that the animals have been exposed to both parasites tested, or horses was exposed to one of the parasites, but cross-reactivity was seen in the IFAT. In case the occurrence of cross-reactivity is verified, there will be support for the use of national isolates in the IFAT at EPM diagnosis.
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14
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LAIS PEREIRA SILVA
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Validation of a bioanalytical method to evaluate the influence of ivermectin on the pharmacokinetics (pk) of carboplatin - preclinical test
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Leader : ALESSANDRA ESTRELA DA SILVA LIMA
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MEMBRES DE LA BANQUE :
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ALESSANDRA ESTRELA DA SILVA LIMA
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FRANCINE JOHANSSON AZEREDO
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STELLA MARIA BARROUIN MELO
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TIAGO DA CUNHA PEIXOTO
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Data: 25 févr. 2019
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Afficher le Résumé
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The number of studies aimed at elucidating the new therapeutic guidelines is increasingly increasing for mammary neoplasms in bitches. In this context, a study was carried out to validate the carboplatin analysis method in the plasma of rats and women to evaluate the influence of proliferation and cyclophosphamide on the pharmacology of carboplatin in healthy wistars - preclinical medical test. We studied the 24 wistars, divided into three experimental groups, Group 1 consisting of animals with carboplatin alone (n = 8), Group 2 animals treated with carboplatin preceded by applications of Ivermectin and Group 3 animals treated with carboplatin associated with cyclophosphamide in metronomic regimen (n = 8). It was collected after general anesthesia of all animals at the same times 0, 5, 15, 30, 45, 60, 120 and 240 minutes after administration of carboplatin, where the animals were euthanized and referred to the pathology sector. The following were collected: kidneys, intestine, fragments of liver and lung. Blood samples were directed to the pharmacokinetic analysis using the noncompartmental model HPLC-UV. The method had full validation in bitches, as this study is a pilot for evaluation of pharmacokinetics in female dogs with mammary carcinomas. The same method was linear and reproducible for plasma analyzes of Wistars rats (pilot study). The data were a modest trend to increase the shelf-life of the ivermectin group when it became a pilot group (carboplatin), which may also be noted in reducing clearance of the drug in the same group. With the result of the histopathological, we did not detect only cases of discrete circulatory and degenerative alterations in the renal and hepatic parenchyma.
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15
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ROSECLEA CHAGAS DOS SANTOS
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HISTOPATHOLOGICAL ASPECTS IN THE KIDNEYS AND EYE GLOBS IN NATURALLY ACQUIRED CANINE LEISHMANIASIS
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Leader : STELLA MARIA BARROUIN MELO
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MEMBRES DE LA BANQUE :
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STELLA MARIA BARROUIN MELO
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FLAVIANE ALVES DE PINHO
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GERALDO GILENO DE SÁ OLIVEIRA
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RICARDO JORGE LEAL SILVESTRE
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Data: 17 mai 2019
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Afficher le Résumé
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The mechanisms of injury associated with chronic systemic inflammatory response are determinant in the clinical manifestations of renal and ocular disease in canine leishmaniasis (CanL) caused by Leishmania infantum. Considering the hypothesis that ophthalmic and renal lesions resulting from inflammation associated with vascular deposition of circulating immune-complex may be quantitatively and qualitatively equivalent to each other, the present study consisted of the comparative evaluation of clinical and pathological findings in dogs in different clinical stage of naturally acquired CanL. Thus, the objective of this study was to examine the histopathological changes in the kidneys and eyes of dogs that evolved to natural death or underwent euthanasia, to determine the presence and possible equivalence of changes in these organs and relation with the severity of the clinical stage. We studied 15 dogs domiciled in endemic areas for zoonotic transmission by L. infantum, whose infection was confirmed by direct microscopy and / or polymerase chain reaction (PCR) in samples of bone marrow and / or splenic aspirates, respectively. The dogs were evaluated by physical examination and clinicopathologic, classified in clinical stages 1 to 4 according to the standardized criteria for the definition of the severity of CanL by the international group LEISHVET, and divided into two experimental groups. Group 1 (G1 = mild-moderate disease) was composed of seven dogs in stages 1 and 2 of CanL and whose biological samples were transferred by another research group after euthanasia; Group 2 (G2 = severe-terminal disease) was composed of eight dogs in stages 3 and 4, who had been treated for CanL but who progressed to natural death due to worsening of the clinical condition or were submitted to euthanasia due to intractable disease and / or unfavorable prognosis. Fragments of kidneys and ocular bulbs were collected and processed for histopathological analysis by light microscopy. The severity of lesions present in the kidneys and eyes was categorized into gradations of 1 to 4 according to criteria of intensity of histopathological changes, for comparative analysis. G1 dogs presented milder renal lesions than those found in G2, whose histopathological changes were more intense and frequent (p = 0.002). In G2 dogs, the renal histopathological changes observed were characteristic of chronic disease, such as interstitial fibrosis, tubular atrophy and segmental sclerosis with glomerular basement membrane adhesion. Examination of the bulb of eye revealed that uveitis was the most commonly observed alteration, present in 80% (12/15) of the cases. There was a higher frequency and greater intensity of histopathological changes in the ocular bulbs of G1 dogs, which were not treated, compared to G2 dogs (p = 0.033) with more advanced stages of the disease, which in the present study had previous treatment history for CanL. The less frequent findings of ophthalmopathies in the group of dogs that progressed to severe disease even after a history of treatment suggest that the therapy may have been effective in reducing ophthalmic disease, but not for CanL-associated kidney disease; further clinical studies should be developed to examine such a clue.
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16
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DANIELA MACHADO CARAPIÁ
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EVALUATION OF THE INTRODUCTION OF RAW BOVINE MEAT IN FEEDING HEALTHY DOGS
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Leader : STELLA MARIA BARROUIN MELO
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MEMBRES DE LA BANQUE :
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STELLA MARIA BARROUIN MELO
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ALESSANDRA ESTRELA DA SILVA LIMA
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CARLOS PASQUALIN CAVALHEIRO
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EDERLAN DE SOUZA FERREIRA
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Data: 20 mai 2019
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Afficher le Résumé
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There is a tendency to seek more natural habits on the part of the population and this tendency includes the feeding practices for their pets; in this case, raw meat foods represent the natural alternative for dogs and cats. The present study proposed to evaluate the potential risks and benefits of raw meat intake as part of the dog diet. Twelve dogs were evaluated, six (6) of commercial kennel and six (6) of kennel-shelter, by means of clinical examination, hematological, biochemical and parasitological analyzes before and after the dietary intervention with 20% of raw bovine meat of the volume ingested. Dogs received the same dry commercial feed for 15 days (adaptation phase) and then received 20% of the daily intake of fresh raw meat for 15 days. The raw beef supplied to the dogs was analyzed by means of microbiology to investigate bacterial contamination using standard methods for the research of mesophilic aerobes, coliforms and Salmonella sp. Samples of raw beef from different commercial establishments in the Metropolitan Region of Salvador, Bahia, were also analyzed by the same microbiological methods. The results of the hemogram revealed increased mean red blood cell counts (p <0.05), globular volume (p <0.05) and hemoglobin (p <0.05) after the introduction of raw meat. There were no significant differences in counts of total, segmented leucocytes, eosinophils, lymphocytes and platelets that could be associated with the diet based on raw meat. The research and quantification of mesophilic aerobic microorganisms indicated that 75% of the meat samples had counts greater than 10 5 UFC, indicating degeneration of the food, 92% of the samples contained enterobacteria, including E. coli, and none contained Salmonella spp. The present study showed that the ingestion of raw meat brought benefits evidenced in the hematological parameters of the dogs. There were no evidence of infection in clinical parameters, leukogram and serum biochemistry and parasitological tests of the dogs that could be associated with the ingestion of raw meat. These results indicate that the use of raw meat in the diet may be beneficial to dogs' health and open the prospect for new studies on nutritional aspects and canine health associated with raw meat feeding
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17
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ÍSIS BARBOSA COSTA
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Cytotoxic evaluation of the mammea A/BB to glioma cells.
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Leader : ALESSANDRA ESTRELA DA SILVA LIMA
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MEMBRES DE LA BANQUE :
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ALESSANDRA ESTRELA DA SILVA LIMA
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KARINE ARAUJO DAMASCENO
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TIAGO DA CUNHA PEIXOTO
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VICTOR DIOGENES AMARAL DA SILVA
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Data: 27 juin 2019
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Afficher le Résumé
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Glioblastoma is the most aggressive form of gliomas and the available therapeutic resources are limited and involve surgical resection, radiotherapy and chemotherapy. The search for more effective and less aggressive pharmacological alternatives places mammea A/BB under analysis. This plant metabolite is found in the bark of roots of the botanical family Calophyllaceae, of the genus Kielmeyera. The objective of this study was to evaluate the cytotoxic potential of mammea A/BB in human glioblastoma U251 and C6 rat glioma cells. The pharmacological action of mammea A/BB was tested in both strains by cell viability assays by MTT, migration test, counting of pycnotic nuclei and quantification of p-glycoprotein expression. The effect of the drug on normal murine astrocytic cells and a comparison of cell viability between the test drug and the first-line chemotherapy, temozolomide, were also analyzed. The experiments showed that the U251 and C6 strains had a concentration dependent sensitivity to mammae A/BB and resistance to temozolomide in the concentration range of 2 μM to 200 μM in all the analyzed samples. The concentrations of 27 ± 2.31 μM and 56.85 ± 14.17 μM of mammea A/BB were able to inhibit the cell population in 50% in the lines U251 and C6, respectively, as well as the migratory potential of these lines. The test drug shows no cytotoxic action on murine astrocyte cells. Pinocytic nuclei were found in greater quantity in the treatment with mammea A/BB when compared to the control and DMSO conditions. Mammea ABB showed no significant difference in the induction of P-glycoprotein expression. The results indicate a potential pharmacological action in the induction of cytotoxicity of the drug in tumor cell types and non-cytotoxic in the normal cells evaluated.
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18
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VANESSA BONFIM DA SILVA
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Development of goat's cheese seasoned with alcoholic beverages.
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Leader : MARION PEREIRA DA COSTA
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MEMBRES DE LA BANQUE :
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LEONARDO FONSECA MACIEL
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MARION PEREIRA DA COSTA
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MAURICIO COSTA ALVES DA SILVA
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MELISSA HANZEN PINNA VALENTIM
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Data: 28 juin 2019
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Afficher le Résumé
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In 2014, Brazil produced 153,659 tonnes of goat's milk, the Northeastern region being the most prominent, accounting for 92% of the Brazilian goat herd. However, goat's milk still has a restricted industrialization in the country, and a greater incentive is needed both for increased consumption and for increased industrialization, which are negatively influenced by the lack of diversity of goat milk products. Thus, an alternative to optimize the dairy goat market is to increase the diversity of products offered by adding value. Goat Coalho cheese seasoned with alcoholic beverages is a great choice for technology improvement and diversification of the goat milk market. Thus, the objective of this study was to develop new Coalho cheeses seasoned from goat's milk using different types of alcoholic beverages (aged cachaça, cocoa honey liquor, mead and pure malt beer), evaluating the physicochemical characteristics, bacterial and sensory characteristics of the new dairy products throughout the storage period. For this, goat's Coalho cheeses seasoned with alcoholic beverages and physical-chemical analyzes (fat, moisture, pH, color and instrumental texture) and bacteriological (lactic acid bacteria count and Lactococcus lactis ssp. cremoris and Lactococcus lactis ssp. lactis count) were performed at day 0 and throughout the storage period; and sensory analysis (consumer expectation, acceptability, ideal scale, purchase intention and check-all-that-apply) to evaluate the characteristics of each formulation. The seasoning of the cheeses with alcoholic beverages, as well as the stage of this process influence in the physical-chemical, bacteriological and sensorial characteristics of the cheeses, and these become softer and more susceptible to deformation during the storage period. The best cheeses seasoning technology was obtained from pressing for 4 hours and seasoning after maturation of the cheeses for 3 days. The expectation of potential consumers over a goat Coalho cheese seasoned with alcoholic beverages is between the nor will not disgustará, expects to be slightly familiar with goat dairy products and expects to enjoy moderately the goat's characteristic flavor. On the seasoning of cheeses with alcoholic beverages, they expect to be slightly familiar with this seasoning and expect to enjoy moderately the taste of alcoholic beverages in cheeses. Among the evaluated treatments, the control had the best acceptability and purchase intention, a result that is associated with the alcoholic content present in seasoned cheeses. All treatments were close to the ideal by the ideal scale and the soft and white characteristics were the most emphasized by the taste testers, agreeing with the results of instrumental color and texture. According to the principal component analysis (PCA), acid and alcohol aromas, acid taste and alcohol flavor were the characteristics responsible for acceptance of the cheese in the acceptability test. In virtue of these results, it can be concluded that it is possible to elaborate goat cheese flavored with alcoholic beverages and these have good acceptability, as well as changes in their physicochemical and bacteriological characteristics.
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19
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JULIA LIGER DE FREITAS
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Causes of death and correspondence between clinical diagnosis and necropsy findings in 1,355 dogs: a retrospective study (2005-2017).
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Leader : TIAGO DA CUNHA PEIXOTO
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MEMBRES DE LA BANQUE :
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ARIANNE PONTES ORIA
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PAULA VELOZO LEAL
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TIAGO DA CUNHA PEIXOTO
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VIVIAN DE ASSUNÇÃO NOGUEIRA CARVALHO
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Data: 30 août 2019
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Afficher le Résumé
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FREITAS, J. L. Causes of death and correspondence between clinical diagnosis and necropsy findings in 1,355 dogs: a retrospective study (2005-2017). 2019. 75 p. Dissertação (Mestre em Ciência Animal nos Trópicos) - Escola de Medicina Veterinária e Zootecnia – Universidade Federal da Bahia, 2019.
The objective of this study was to determine the frequency of diseases that culminated in death or justified the euthanasia of dogs necropsied at the Veterinary Pathology Laboratory of the Federal University of Bahia (2005-2017), as well as the frequency of zoonoses and median age of death (MAD). A total of 1.355 dog necropsy protocols were analyzed and epidemiological information and anatomopathological diagnosis were obtained. The cause of death could be determined in 86.79% of the cases and in 13.21% the diagnosis was inconclusive. The diagnosed diseases were grouped in 10 categories, being observed the following frequency: parasitic infectious diseases (24.13%), neoplasms (22.58%), degenerative diseases (10.85%), disorders caused by physical agents (8.93%), poisoning and toxiinfections (6.49%), other disorders (5.17%), metabolic and endocrinological disorders (4.06%), iatrogenic disorders (2.88%), developmental disorders (1.48%) and immune-mediated diseases (0.22%). Infectious and parasitic diseases were the main causes of death in puppies (44.62%) and adults (26.52%). On the other hand, among the elderly, most deaths were related to neoplasms (42.37%). Canine Distemper (10.46%), CKD (6.9%), running over trauma (5.98%), Pyometra (5.29%), Carbamate Poisoning (5.06%), Canine Visceral Leishmaniasis (4, 83%), leptospirosis (4.71%) and parvovirus (4.60%) stood out among the most prevalent conditions. There was a high frequency of zoonoses (10.43%) and a high death rate caused by diseases that could be prevented (15.06%) by vaccination (distemper and parvovirus). Of the evaluated animals, 49.96% had natural death, while 48.04% underwent euthanasia, whose main motivation was the neoplasms (30.41%). The MAD in the population studied was eight years old, and females and medium-sized animals had significantly higher MAD. Among the breeds, the Cocker Spaniel had the highest MAD (12 years). The results obtained in this study, in particular the knowledge of the main diseases responsible for dog death, may help in the establishment of differential diagnoses, adoption of prophylactic measures, guidance of tutors and training of veterinarians from Salvador and municipalities of the interior of Bahia.
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20
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RAFAELA DE ALMEIDA OLIVEIRA
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Analysis of barriers in eggs and backyard chickens trading with family farmers settiers of land reform at Salvador Metropolitan Area, Bahia, Brazil.
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Leader : CARLOS ROBERTO FRANKE
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MEMBRES DE LA BANQUE :
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CARLOS ROBERTO FRANKE
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JOAO AURELIO SOARES VIANA
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LIA MUNIZ BARRETTO FERNANDES
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LUIS FERNANDO BATISTA PINTO
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Data: 23 sept. 2019
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Afficher le Résumé
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The objective of this research is identify the critical restrictions on poultry rearing, and obstacles on backyard chicken and eggs production and trading. Based on previous experiences described by GESAV in communities located at rural areas, it was noted the need to identify management constraints that restricts the endeavor market, which reduce the chances of improvement in the income of these families. The study was realized in rural agrarian reform settlement, on Dias d'Ávila. Participatory methodology was proposed, valuing popular knowledge and customs. Through the dialogue and interviews with the breeders, the socioeconomic profile and characterization of the activity of those involved in family poultry production were traced. The results were tabulated and analyzed using the qualitative methodology Discourse of the collective subject (DSC), to identify the main ideas and determine hindrances. Through the proposed methodology, it was identified some operational limiting points.
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21
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ANA PAULA GOES COELHO
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Transversus abdominis plane block associated to intraperitoneal analgesia with levobupivacaine, compared to epidural use in female dogs undergoing ovariohysterectomy, anesthetized with propofol.
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Leader : VIVIAN FERNANDA BARBOSA
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MEMBRES DE LA BANQUE :
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ANTONIO DE LISBOA RIBEIRO FILHO
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CATERINA MURAMOTO
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VANESSA BASTOS DE CASTRO SOUZA
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VIVIAN FERNANDA BARBOSA
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Data: 24 sept. 2019
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Afficher le Résumé
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GOES COELHO, A. P. G. Transversus abdominis plane block associated to intraperitoneal analgesia with levobupivacaine, compared to epidural use, in female dogs undergoing ovariohysterectomy, anesthetized with propofol. 2019. 80p. Dissertação (Mestre em Ciência Animal nos Trópicos) – Escola de Medicina Veterinária e Zootecnia – Universidade Federal da Bahia, 2019. Locoregional anesthesia, as a perioperative analgesic strategy, provides decrease in anesthetic requirements and post-surgical analgesia. The aim of this study was to compare the cardiorespiratory effects, anesthetic sparing and analgesic efficiency of levobupivacaine used to ultrasound-guided transverse abdominal (PTA) block, associated to intraperitoneal instillation, versus epidural use, in female dogs undergoing ovariohysterectomy (OH), anesthetized with propofol. Fourteen healthy adult dogs, premedicated with acepromazine maleate (0.03 mg / kg) and pethidine hydrochloride (3 mg / kg), intramusculaly, were used. Propofol was used for induction (5 mg / kg) and anesthetic maintenance, under spontaneous ventilation (FiO2 = 1.0). Dogs were assigned into two groups (n = 7): the GA group received bilateral levobupivacaine (1 mg / kg to 0.25%) in the PTA ultrasound-guided block and by intraperitoneal instillation and the GB group was treated with the same drug (2mg / kg to 0.5%) by epidural route (0.1 ml / cm of the occipito-coccigen distance). After 20 minutes, OH was performed. Heart rate (HR), respiratory rate (f), diastolic systolic blood pressure (DBP) and mean (MAP), partial oxygen saturation in hemoglobin (SpO2) and final expired concentration of carbon dioxide (ETCO2) was evalluated at supramaximal stimulus moments: end of celiotomy (M2); clamping of the right (M3) and left (M4) ovarian pedicles; clamping of the uterine cervix (M5); musculature (M6), subcutaneous (M7) and skin (M8) rafias. During postoperative period, the quality of anesthetic recovery and the time between the end of the anesthesia and: extubation (EX), head movement (MC), sternal position (PE) and quadrupedal position (PQ), was registered; Pain assessment was performed according Melboume University Pain Scale (EDUM) for six hours-postoperative (M30, M60, M90, M120, M180, M240 and M360). The treatments determined similar propofol consumption, detecting higher mean HR in GB (M2, M4, M6, M7 and M8). In M1, lower values of PAM and PAD for GB and lower mean values of f for GA were found. The quality of recovery was regular in both treatments. Higher MC (p = 0.008) and PE (p = 0.024) intervals were detected for GA, in addition to higher pain scores at 90 minutes postoperatively. No analgesic rescues were necessary. Muscle tremors, urination, defecation, vomiting and nausea were observed without significant variation between groups. Levobupivacaine in bilateral ultrasound-guided transverse abdominis plane block associated to intraperitoneal instillation may be an alternative to epidural route, as part of multimodal anesthetic protocols for intra-abdominal surgeries, determining similar postsurgical analgesia. Both techniques ensure cardiorespiratory stability and determine similar effects on propofol consumption, however, result in a high incidence of vomiting and muscle tremors.
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22
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ELAINE CRISTINA DE FREITAS OLIVEIRA
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Zoonoses, vector diseases and accidents with venomous animals in Brazilian Army military (2017 and 2018).
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Leader : CARLOS ROBERTO FRANKE
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MEMBRES DE LA BANQUE :
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RICARDO WAGNER DIAS PORTELA
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CARLOS ROBERTO FRANKE
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AROLDO JOSE BORGES CARNEIRO
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ARTUR GOMES DIAS LIMA
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Data: 25 oct. 2019
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Afficher le Résumé
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For the Brazilian Army to fulfill its missions, the approximately 217,000 military personnel are constantly being trained and exposed to biological hazards. The objective of this work was to know the biological risks in Brazilian military activities and to specify what are the prevention and control measures of the identified risks. The Army Health Directorate was required to report compulsory military disease data from 2017 and 2018, and the incidence of injuries was calculated. Information on notification problems was collected through consultations with electronic websites that provide information from the Notification Disease Information System, 2017 and 2018, and the incidences of notified diseases were calculated. A comparison was made between the incidence of injuries in the military and the general population to identify whether the risk of illness was higher among the military. A consultation was made with the Supply Directorate and a survey of animal-based military organizations to identify cases of zoonoses. To specify the prevention and control measures of the identified diseases, a bibliographic search was made in the academic databases. The results show that urban arboviruses transmitted by the mosquito Aedes spp. It is the diseases that had more notifications among the military and it was found that in some diseases the risk of getting sick is higher in the military than in the general population. Although cases of zoonoses have been reported in military animals, they have not served as a reservoir for the disease. The prevention and control measures for identified health problems are individual protection measures, environmental control measures, immunization, chemoprophylaxis and health education, in addition to the fundamental work of the health officer in risk assessment and proposition of prevention and control measures and the participation of commanders in ensuring that the troops will implement the measures.
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Thèses |
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1
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ANA CLAUDIA SANTOS RAPOSO
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QUALITATIVE EVALUATION OF TETRAPHOR TEARS
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Leader : ARIANNE PONTES ORIA
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MEMBRES DE LA BANQUE :
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ARIANNE PONTES ORIA
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CARLOS ROBERTO FRANKE
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RODRIGO BARBAN ZUCOLOTO
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THIAGO LUIZ DE PAULA CASTRO
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VITOR HUGO MOREAU DA CUNHA
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Data: 19 févr. 2019
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Afficher le Résumé
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Tear is a complex fluid involved in nourishment, stability, protection and removal of foreign bodies from ocular surface. There are studies regarding its components and dynamics for mammalian species; however, the knowledge about this fluid in other species is limited. The evolutive process for adaptation and environmental influences may inducted changes on vertebrate tears. In addition, qualitative and quantitative differences can occur, even in animals phylogenetically related. Therefore, the objective of this research was to perform qualitative-quantitative evaluation of tetrapod tears (reptiles, birds and mammals), using unpublished methodologies for wild animals. The protein profile and biochemical composition of the tear of reptiles, birds and mammals, present in different ecological niches, were evaluated comparatively with blood serum and human tear, and it was obtained that the tear of the animals contained the same compounds present in the tear human and blood serum in different proportions. Together with this finding, it was attributed that the phylogenetic proximity, diet and environment, the latter in a significant way, influence on the tear components. From these results, some species were selected because of their notorious differences in the electrophoretic profile for the proteomic investigation of the tear, as hawk (Rupornis magnirostris), caiman (Caiman latirostris) and sea turtle (Caretta caretta). The proteins found, as well as their ontogenic characteristics, have demonstrated that the tear composition can be derived from the environmental conditions and lifestyles of the animals, being able to adapt to the complex adaptation of the species. Besides, even before the most primitive metabolic processes, there is an maintenance in the stability mechanisms of tears. So, the tear fluid pervades what is described for mammals and is a fluid with high adaptation power. It has roles in maintaining ocular surface homeostasis and consequently visual function.
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2
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ADRIANA LEBRAM VON SOHSTEN
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Human, avian and soil contamination by Toxocara spp in a rural community of Feira de Santana, Bahia.
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Leader : ARISTEU VIEIRA DA SILVA
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MEMBRES DE LA BANQUE :
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ARISTEU VIEIRA DA SILVA
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CARLOS ROBERTO FRANKE
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LIA MUNIZ BARRETTO FERNANDES
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SILVANE MARIA BRAGA SANTOS
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ARTUR GOMES DIAS LIMA
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Data: 20 févr. 2019
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Afficher le Résumé
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Toxocariasis is a neglected geohelmintosis caused by Toxocara canis and T. cati, own of the dog and the cat, respectively. Paratenic hosts, including humans, may become infected by ingesting eggs with larvae in the soil, water, food and animals. Occult toxocariasis is the most common in man but may occur visceral larva migrans (liver and other organs), ocular larva migrans, and neurological changes. The objective of this research was to study the occurrence of Toxocara spp in humans from a rural community in Feira de Santana, Bahia, to evaluate the factors associated with human infection and environmental contamination, as well as verify the possibility of broilers raised extensively as indicators of ground contamination. A total of 425 human serum samples were collected and tested for serum anti-Toxocara spp antibodies by ELISAi, with 15.76% seropositive. Samples of 400g of soil from each of 15 properties were analyzed for trace element detection and determination of clay types, relating these data to the amount of parasite eggs per soil gram, the degree of plant cover, climatic and epidemiological (related to the presence of dogs and cats in properties) variables. The analysis of multivariate models of association of variables determined that the number of dogs was significantly associated with environmental contamination by Toxocara spp. Chickens from pathogen-free farms were used as indicators of environmental contamination, with 18 birds being released at 15 days of age on property with a history of dogs with Toxocara infection, having the blood collected at 0, 15 and 30 days after the release. Serum anti-Toxocara spp antibodies examination by ELISAi resulted in increasing reactivity indices. Soil samples revealed a higher frequency of embrionated than infertile Toxocara eggs. At the end of the experiment, the intestinal contents were obtained for coprological examination of nine of these birds. Eggs of Ascaridia galli were found in all nine birds examined and in five of these were recovered adults of A. galli. It is demonstrated the infection of people, the epidemiological characteristics capable of maintaining Toxocara eggs and the potential of birds created extensively as markers of environmental contamination.
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3
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LUCIANA BAHIENSE DA COSTA
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The Epidemiology of Visceral Leishmaniasis in the State of Bahia
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Leader : CARLOS ROBERTO FRANKE
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MEMBRES DE LA BANQUE :
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AROLDO JOSE BORGES CARNEIRO
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ARTUR GOMES DIAS LIMA
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CARLOS ROBERTO FRANKE
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MANUELA DA SILVA SOLCA
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STELLA MARIA BARROUIN MELO
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Data: 20 févr. 2019
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COSTA, L. B. Epidemiology of visceral leishmaniasis in Bahia. 2018. Tese (Doutorado Ciência Animal nos Trópicos) - Escola de Medicina Veterinária e Zootecnia – Universidade Federal da Bahia, 2018.
The study of visceral leishmaniasis (VL) over 11 years (2007 to 2017), with an emphasis on children under 10 years of age, besides favoring the behavior and expansion of VL in the State of Bahia allows the evaluation of strategies developed and the possible impact in the control of the grievance. For the present study, data from SINAN were collected throughout the State and in seven municipalities with two or more deaths in children under 10 years of age between the years of 2017 and analyzed: univariate descriptive cases and deaths by LV in the historical series, as well as spatialization of incidence and mortality by LV in the total population and between zero and nine years of age, and multivariate and logistic regression among socioeconomic indicators (HDI, IPESE, TU, dryness and Gini coefficient) and occurrence of cases and deaths, and between sex, age, ethnicity, schooling and time between onset of symptoms and treatment, in individuals with evolution to cure and death. In pre-selected municipalities with two or more VL deaths, after an epidemiological questionnaire, sampling in individuals and dogs, entomological research and geo-referencing of health units, a descriptive analysis of the current situation of the outbreak and analysis of risk and perception factors of the population regarding the aggravation. In the State, during the study period, it can be observed after adjusting the logistic regression model that the higher the age (pvalor 0.000 * CI 5% / 95% C) and the higher the income inequality (pvalor 0.000 * CI 5% / 95 % C) the higher the chance of death due to LV. In the pre-selected municipalities we can observe unfavorable environmental and housing conditions in the death and cure groups, as well as risk factors for VL, including the presence of phlebotomines and positive dogs, but the perception about the disease was higher in the group of cure. The prevalence among dogs in the residences and neighborhoods, and individuals in both groups was 0.19, 0.15 and 0.019, respectively, and the sandfly species L. longipalpis and L. lenti in the majority of catches in the peridomicile, with presence of females of the vector in both groups, highlighting the transmission still active in most of the researched locations. The characterization of the aggravation in the State, as well as the identification of possible associations with socioeconomic and environmental indicators, and the identification of risk factors associated with deaths in children under 10 years makes research relevant, providing subsidies for the evaluation of conduct and possible redirection of control strategies in the State of Bahia.
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4
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CARLOS HUMBERTO DA COSTA VIEIRA FILHO
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Characterization of the inflammatory response and correlation of MiTF and p38MAPK expression with prognostic factors in canine melanomas.
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Leader : ALESSANDRA ESTRELA DA SILVA LIMA
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MEMBRES DE LA BANQUE :
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ALESSANDRA ESTRELA DA SILVA LIMA
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TIAGO DA CUNHA PEIXOTO
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EDUARDO LUIZ TRINDADE MOREIRA
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GEOVANNI DANTAS CASSALI
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KARINE ARAUJO DAMASCENO
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Data: 25 mars 2019
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The response of melanoma-associated lymphocytic inflammatory infiltrate has been studied over the years in medicine as a possible prognostic factor for this highly aggressive tumor, but this evaluation is still not well described in canine melanomas. Thus, in this work we aim to characterize the inflammatory response associated with canine melanomas in the different anatomical sites (mouth, finger and skin) and verify the expression and correlation of MiTF and p38MAPK with prognostic factors. We studied 117 melanomas classified based on their location in two groups: melanomas located in oral cavity / digits (n = 74) and cutaneous melanomas (n = 43), which were submitted to morphological and morphometric analysis of inflammatory cells and clinical evaluation systematic, in addition to evaluating the survival rate of the animals. From these parameters, it was observed that oral tumors / digits were associated with pathological features related to the worst prognosis such as size, mitotic index, histological type, nuclear atypia and the presence of metastasis. The inflammation was more present in the oral / digits tumors, however discrete, when compared to the group of cutaneous melanomas, the lymphocytes being the predominant cell type. The multivariate analysis showed that the presence of lymphocytic infiltrate was an independent prognostic factor, correlating with the survival rate. These results may help to better understand the role of inflammatory infiltrates, especially lymphocytic ones, in canine melanomas, providing alternative tools in establishing possible prognostic factors.
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5
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MARÍLIA CARNEIRO DE ARAÚJO MACHADO
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Influence of cyclophosphamide and pharmacokinetics of carboplatin on female dogs with mammary carcinomas and degree of adverse effects of chemotherapy
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Leader : ALESSANDRA ESTRELA DA SILVA LIMA
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MEMBRES DE LA BANQUE :
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ALESSANDRA ESTRELA DA SILVA LIMA
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STELLA MARIA BARROUIN MELO
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FRANCINE JOHANSSON AZEREDO
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GEOVANNI DANTAS CASSALI
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KARINE ARAUJO DAMASCENO
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Data: 26 mars 2019
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There is increasing number of studies that seek new therapeutic approaches for mammary neoplasia in female dogs. In this context, the objective of this study was to evaluate the influence of cyclophosphamide on the pharmacokinetics (PK) of carboplatin in female dogs with mammary carcinomas, as well as to verify possible side effects associated with the treatment. Sixteen female dogs, all with histopathological diagnosis of mammary carcinomas, were divided into two experimental groups: Group 1 consisted of animals treated with carboplatin alone in the conventional chemotherapy modality (n = 8) and Group 2 animals treated with carboplatin as conventional chemotherapy associated with cyclophosphamide in metronomic regimen (n = 8). All animals were submitted to clinical evaluation, mastectomy, chemotherapy with carboplatin and pharmacokinetic analysis, as well as the evaluation of survival rate and adverse effect score periodically. The results showed that although there was a significant difference in carboplatin pharmacokinetic parameters (volume of distribution, half-life, clearance and area under the curve), there was a significant difference in the reduction of hematological values for the dogs treated with carboplatin + cyclophosphamide, however these were not sufficient to alter the degree of adverse effects caused by this type of treatment. These results evaluated together indicate that the association between carboplatin and cyclophosphamide represents a viable alternative for the adjuvant treatment of female dogs with mammary carcinomas, since it contributes to the increase in the survival rate of these patients.
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6
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GABRIEL FELIPE OLIVEIRA DE MENEZES
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Use of dimethylacetamide and dimethylformamide associated with glycerol or not for cryopreservation of ovine semen
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Leader : ANTONIO DE LISBOA RIBEIRO FILHO
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MEMBRES DE LA BANQUE :
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ANTONIO DE LISBOA RIBEIRO FILHO
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CLARISSE SIMOES COELHO
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RODRIGO FREITAS BITTENCOURT
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ANA KARINA DA SILVA CAVALCANTE
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MARIA MADALENA PESSOA GUERRA
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Data: 29 mars 2019
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The objective of this work was to evaluate the effect of cryoprotectants dimethylacetamide (DMA) and dimethylformamide (DMF) associated or not to glycerol (GL) used in the formulation of diluent media for cryopreservation of ovine semen in vitro sperm parameters and fertility in vivo. For that, two experiments were carried out with seven treatments each. In the experiment 1 the objective was to evaluate the effect of the use of dimethylacetamide, in different concentrations, associated or not to glycerol, for the preparation of diluents for sperm cryopreservation, on sperm parameters and fertility index of post-thaw sheep semen. The treatments were divided into G1: GL6%; G2: DMA3%; G3: GL5% + DMA1%; G4: GL4% + DMA2%; G5: GL3% + DMA3%; G6: GL2% + DMA4% and G7: GL1% + DMA5%. Experiment 2 had the objective of studying the efficiency of dimethylformamide in different concentrations in the dilution media, associated or not to glycerol, for the maintenance of ovine sperm viability in vitro and in vivo after thawing. The groups were divided into G1: GL6%; G2: DMF3%; G3: GL5% + DMF1%; G4: GL4% + DMF2%; G5: GL3% + DMF3%; G6: GL2% + DMF4%; G7: GL1% + DMF5%. Samples from both experiments were evaluated after thawing, for sperm kinetics through computer assisted sperm analysis and membrane integrity by flow cytometry. The results were analyzed through the Statistical Analysis System - SAS with significance level of 5%. No difference (P> 0.05) was observed between the treatments of experiment 1 using DMA in the in vitro and in vivo evaluations. In experiment 2 with DMF, there was difference in membrane integrity only between G4 and G7 (P <0.05), which was not demonstrated in the in vivo test. It was concluded that cryoprotectants based on dimethylacetamide and dimethylformamide were efficient in the cryopreservation of ovine semen and could be used as an alternative cryoprotectant.
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ROGERIO FERNANDO DE JESUS
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ANTIBODIES AGAINST Toxoplasma gondii E Sarcocystis spp. PRODUCED BY EXPERIMENTAL INFECTION IN RODENTS: CHARACTERIZATION AND EVALUATION OF CROSS REACTIVITY WITH CORRELATED PROTOZOAN
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Leader : LUIS FERNANDO PITA GONDIM
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MEMBRES DE LA BANQUE :
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LUIS FERNANDO PITA GONDIM
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RICARDO WAGNER DIAS PORTELA
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THIAGO CAMPANHARO BAHIENSE
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ALEXANDRE MORAES PINHEIRO
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ALEXANDRE DIAS MUNHOZ
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Data: 31 mai 2019
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Toxoplasma gondii and Sarcocystis spp. are cyst-forming parasites of the Apicomplexa phylum excreted by Didelphis spp. While T. gondii is widespread in all regions of the world, infecting several species of mammals and birds, Sarcocystis spp. excreted by opossums are limited to the Americas, accompanying the distribution area of their definitive hosts. Improvement of the serological diagnosis for both parasite genera is essential, aiming both to identify the infective stage involved in cases of human toxoplasmosis, and to evaluate the possibility of cross-reactivity between Sarcocystis spp. phylogenetically correlated. The aims of this study were to characterize monoclonal antibodies against sporozoites of T. gondii with antigen of different stages of the parasite and other related-coccidia; to evaluate the use of gerbil as an experimental host of Sarcocystis falcatula-like strain isolated in Bahia, and to investigate the possible serologic cross-reactivity using Sarcocystis neurona and S. falcatulalike antigens. One of the monoclonal antibodies (G1/19) recognized only T. gondii sporozoite antigen, while the other (K3/7-13) identified tachyzoite and sporozoite proteins. The gerbils were not susceptible to infection by S. falcatula-like, but seroconverted and showed crossreactivity with S. neurona antigen on Immunoblot. These findings indicate that the monoclonal G1/19 is promising for the development of a specific diagnosis for exposure to T. gondii oocysts, as well that cross-reactive occurs between S. falcatula-like and S. neurona in Immunoblot, impacting the interpretation of serological results for this last parasite.
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EVELIN SANTIAGO VASCONCELOS DOS SANTOS
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VASCONCELOS E.S. BACTERIOLOGICAL AND MOLECULAR EVALUATION BY SPOLIGOTYPING OF TUBERCULOSIS FROM BOVINE AND BUBALINOS ABATIDATED IN OFFICIAL PACKAGING AND VESSELS MARKETED IN FREE FAIRS IN THE STATE OF BAHIA - BRASIL.
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Leader : JOSELITO NUNES COSTA
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MEMBRES DE LA BANQUE :
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JOSELITO NUNES COSTA
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JOSÉ DIOMEDES BARBOSA NETO
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LUDMILLA SANTANA SOARES E BARROS
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MAURICIO COSTA ALVES DA SILVA
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TIAGO DA CUNHA PEIXOTO
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Data: 4 juil. 2019
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Afficher le Résumé
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Mycobacterium bovis is the main responsible for bovine TB and buffalo, an important zoonotic disease with global distribution. The precise investigation of this disease, including more advanced diagnoses, like Spoligotyping, allows to choose the best strategies to combat the disease progression. As in other states, understanding the frequency and distribution pattern of the disease in Bahia contributes to effective planning of control actions, as well as monitoring the progress of the national TB control and eradication program and, in this context, slaughterhouses play a key role. The objective was to investigate the occurrence of lesions suggestive of tuberculosis in bovine and buffalo carcasses inspected in slaughterhouses under an official inspection regime, as well as to identify the presence of mycobacteria in the same and in viscera samples marketed in open fairs in the state of Bahia.
Of the 29 free-trade samples, 20 could be analyzed, excluding autolysed and contaminated, and growth was shown in 25% (5/20) of them, but no evidence of BAAR was shown, however, the environment of the fairs was revealed with inappropriate sanitary conditions. A total of 453,417 carcasses were investigated under the state and federal inspection service, of which 31 animals (0.007%) were considered suspect, from which 49 lesions suggestive of TB were collected from animals from 17 municipalities. Of these, 27 cattle could be analyzed resulting in 74.1% (20/27) growth in selective culture medium and 95% (19/20) were AFB. DNA from the colonies was extracted by thermolysis. All isolates were submitted to Spoligotyping with 95% confirmed profiles of M. bovis (SB0120, SB0121, SB0852, SB0828, SB0295, SB0881, SB1648, SB0140, SB0140, SB1055) showing the genotype strain SB0120 to be the most prevalent and related with cases of human tuberculosis indicating the zoonotic potential of this profile. Spoligotyping allowed to confirm the presence of mixed infections, a high diversity of isolate strains patterns in Bahia and also allowed comparison with other states and countries, showing isolated strains in Brazil, one of the isolated isolates in the literature. The analysis of the spatial distribution has proved to be an important tool for directing health protection actions, allowing for more specific recommendations of the national program for the control and eradication of bovine tuberculosis in the state of Bahia.
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