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Disertaciones |
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1
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CARINE SOUZA DA SILVA
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Production of algal bioproducts during the treatment of produced water effluent collected from the oil industry
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Líder : FABIO ALEXANDRE CHINALIA
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MIEMBROS DE LA BANCA :
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FABIO ALEXANDRE CHINALIA
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JOSILENE BORGES TORRES LIMA MATOS
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MILLENO DANTAS MOTA
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Data: 22-ene-2021
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Resumen Espectáculo
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The oil industry can generate a high volume of wastewater associated with oil extraction activities. This effluent is known as produced water and is characterized by a complex composition of petroleum products and salts. This effluent must be treated before being discarded or used in other industrial processes. The objective of this study was to evaluate the treatment of produced water from the oil industry through its use for the cultivation of microalgae. The produced water has essential nutrients for the cultivation of microalgae and its reuse economically increases its value (upcycling). Dunaliella salina was grown in different concentrations of produced water supplemented with the Conway medium, and later went through an adaptation period to produced water with high salts content. The results showed that the culture medium prepared with AP can generate biomass and algal lipid with a reduction of only 2.3 times in relation to the control. However, the process of adapting or acclimating the microalgae to salted AP showed a positive gain for the cultivation of Dunaliella salina, reaching an increase in the specific growth rate of 0.28 and 0.29 d-1, biomass productivity 45 and 48 mg L d-1 and pigment accumulation at salts concentrations of 45 and 50 gSDT / L, respectively. The adaptation process also reduced the cultivation period. It is also noteworthy that during culturing showed a significant removal of inorganic nutrients (N and P), as well as organic load (COD) during the cultivation process (≥ 85%, respectively). Therefore, contributing significantly to the treatment of PA. This research also identifies that the best saline concentration for the cultivation of Dunaliella salina in AP is 50 gSDT / L. This fact facilitates the cultivation process in open and large-scale lagoons, since the selective strength of the high concentration of salts prevents contamination and growth by invasive microalgae.
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2
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BIANCA BOMFIM ANDRADE
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BIOMASS PRODUCTION AND CHARACTERIZATION OF MICROALGAE OBTAINED DURING CULTIVATION WITH RE-USED MEDIUM
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Líder : SUZANA TELLES DA CUNHA LIMA
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MIEMBROS DE LA BANCA :
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EDSON DE JESUS MARQUES
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SUZANA TELLES DA CUNHA LIMA
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TAIARA AGUIAR CAIRES
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Data: 25-feb-2021
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Resumen Espectáculo
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Microalgae are microscopic, prokaryotic or eukaryotic, single-celled and photosynthetic organisms found in marine, freshwater and terrestrial environments. Its rapid growth, diversity of metabolites, ability to adapt to adverse conditions and composition with large amounts of proteins, carbohydrates, pigments, lipids and other biomolecules are responsible for its great biotechnological importance. One of the alternatives for reducing the cost of producing microalgae biomass is the reuse of the culture medium, which consists of the separation of microalgal biomass and the consequent inoculation of the residual medium, which can be supplemented or not with nutrients. Once cultivated, the biomass produced needs to be separated from the culture medium in order to be used in different industrial processes. It is estimated that the cost of separation can reach 20–30% of its total production cost. Electroflocculation is a method of separating biomass that is ecologically correct, inexpensive and energy efficient and consists of the use of two metallic electrodes (an anode and a cathode) that release ions that neutralize the charge of the microalgal cells and allows the formation of cell flakes, resulting in the separation of biomass. Thus, the development of technologies for the production and separation of microalgal biomass that allow cost reduction, without compromising the efficiency and quality of the process, is of great importance for the establishment of biomolecules obtained from microalgae on the world market. Therefore, this work aims to verify the effect of the reuse of medium during the cultivation of marine and freshwater algae, as well as the electrofloculation test as a method for separating the biomass from marine microalgae. For this, the microalgae Dunaliella salina and Ankistrodesmus falcatus were grown in an Erlenmeyer type photobioreactor (1 L) in Conway and LC-Oligo media, respectively. D. salina was submitted to 3 different culture conditions: standard medium, medium supplemented with nitrogen and medium supplemented with phosphorus. At the end of each cycle, the residual medium was reused, receiving a new inoculum of D. salina. The parameters of maximum production, maximum growth rate and maximum productivity were analyzed. There was a reduction in biomass production, maximum growth and productivity during cultivation in the non-supplemented medium and supplemented with phosphorus after 3 cycles. In the medium supplemented with Nitrogen there was a significant increase in the production of biomass in the media reused in comparison to the Control culture. The highest biomass production was obtained in the 1st cycle in a reused medium (1,122 g L-1 ). The 4th cultivation cycle promoted greater biomass production than the 3rd cycle (0.908 g L-1 and 0.854 g L-1 , respectively). The maximum productivity increased significantly during the cultivation cycles in reused medium, with the highest productivity obtained in the 4th cycle (0.201 g L-1 d -1 ). Cell death was observed during cultivation of A. falcatus in reused medium. In addition, an electroflocculation device for a tubular photobioreactor was developed, consisting of two parallel aluminum electrodes and an electrical source with voltage adjustment (12V–24V) for separating microalgae biomass, which resulted in the filing of a model patent used together to the National Institute of Industrial Property.
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3
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VITOR DOS SANTOS ALVES
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MULTIPLEX PCR ASSAY FOR IDENTIFICATION OF MITES IN CULTURE AND ENVIRONMENTAL SAMPLES
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Líder : CARINA DA SILVA PINHEIRO
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MIEMBROS DE LA BANCA :
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BARBARA DE CASTRO PIMENTEL FIGUEIREDO
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CARINA DA SILVA PINHEIRO
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RICARDO WAGNER DIAS PORTELA
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Data: 22-abr-2021
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Resumen Espectáculo
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It is widely accepted that some mites play a central role in triggering allergic manifestations. Among the mites, we can highlight those of the genus Blomia and Dermatophagoides as the most important cause of allergy in Brazil. Mites are routinely cultivated with the intention of producing allergenic extracts for the purpose of diagnosing and treating allergies. Currently, the identification of mites is made based on morphological characteristics; however, this technique is a time-consuming and ambiguous. To overcome these drawbacks, we developed a multiplex PCR (mPCR) assay based on ribosomal DNA (rDNA) capable of identifying the B. tropicalis, D. pteronyssinus and Glycycometus malaysiensis mite species. For this, the complete ITS2 region, flanked by partial sequences of the 5.8 S and 28 S subunits, was amplified by PCR and sequenced. The sequences obtained were aligned with sequences obtained from the Genbank database and used in the design of species-specific primers. Three pairs of primers were evaluated for their sensitivity and specificity and used to compose the mPCR. The mPCR assay was tested on environmental samples, evaluating the frequency of the mites studied in twenty dust samples from homes in the city of Salvador, northeastern Brazil. The data showed that the mites B. tropicalis, G. malaysiensis and D. pteronyssinus, were present in 95%, 70% and 60% of the collected samples, respectively. It was the first time that G. malaysiensis was identified in Brazil and that its ITS2 sequence was made available. Results demonstrate that the mPCR assay proved to be a fast, reliable and straightforward tool for identifying these mites in culture and environmental samples, and can be applied in future epidemiological or diagnostic studies for the mites studied.
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4
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JAIR LUCAS OLIVEIRA JUNIOR
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Biochemical and physiological profiles of maturation, longevity and germination of Brazilian peppertree (Schinus terebinthifolia Raddi) seeds.
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Líder : RENATO DELMONDEZ DE CASTRO
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MIEMBROS DE LA BANCA :
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RENATO DELMONDEZ DE CASTRO
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CIMILLE GABRIELLE CARDOSO ANTUNES
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RENATA SILVA-MANN
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Data: 16-jun-2021
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Resumen Espectáculo
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Brazilian red pepper tree (Schinus terebinthifolius) has been studied mainly for its condiment and medicinal properties, in addition to its importance in the recovery of degraded areas and the restoration of native vegetation. Therefore, due to the importance of obtaining good quality seeds of this species for use in reforestation and commercial planting programs, the objective of this study was to evaluate the biochemical and physiological profiles of seed maturation, storage and germination under conditions of water restriction and after osmopriming. It was observed that the seeds obtained from fruits in the Red stage showed better physiological quality and that the newly harvested seeds showed better germinative performance compared to those that underwent drying. The seeds stored in a refrigerated environment (5ºC) were able to maintain their physiological quality for 15 months, different from those stored at room temperature (24ºC), which remained viable, however, reduced germination and vigor. The changes in the activities of the enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in these storage conditions indicate that the enzymatic antioxidant system is related to the maintenance of the viability of the seeds, however, they were not able to prevent the loss of quality, the increase in lipid peroxidation, verified through the quantification of malondialdehyde (MDA), and the permeability of membranes, through the electrical conductivity (EC) test, when stored at room temperature. In water restriction tests, it was observed that the species is highly susceptible to this abiotic stress in the process of germination and initial seedling establishment. Biochemical evaluations showed that the activities of the enzymes SOD, CAT and APX during controlled imbibition increased when subjected to low levels of stress (-0.2 MPa), however, they were reduced in severe levels of water restriction (-0.8 MPa). In seedlings, the first oxidative damage occurred in the roots, confirmed by the reduction of the activity of SOD, CAT and APX by -0.2 MPa. In addition, when using controlled imbibition for osmoconditioning or priming, it was observed that the use of potentials less than -0.2 MPa for 168 h did not promote improvement in the germination and vigor of the seeds of S. terebinthifolius, however, potentials of -0.2 and -0.4 MPa, were able to promote a faster and more uniform germination, indicating that the use of this pre-germinative treatment can contribute to improve the physiological potential of seeds of this species.
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5
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CAROLINA DE ARAÚJO ROLO
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MOLECULAR DETECTION OF EMERGING ARBOVIRUS: WEST NILE, SAINT LOUIS AND MAYARO
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Líder : GUBIO SOARES CAMPOS
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MIEMBROS DE LA BANCA :
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GUBIO SOARES CAMPOS
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LUIS GUSTAVO CARVALHO PACHECO
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ACÁSSIA BENJAMIN LEAL PIRES
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Data: 04-nov-2021
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Resumen Espectáculo
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Arboviruses are viral diseases transmitted by arthropod vectors, especially mosquitoes of the Culex genus. These diseases are spread across the world and have major impacts on health and the economy. In Brazil there are several arboviruses in circulation, such as Zika (ZIKV) and dengue (DENV). Some arboviruses are considered emerging, such as mayaro (MAYV), Saint Louis encephalitis virus (SLEV) and West Nile virus (WNV). Despite having been detected in Brazil and having the potential to cause epidemics, these arboviruses have not yet spread within Brazilian territory. Due to geographic and social characteristics, Brazil presents the ideal environment for the dispersion of arboviruses. In this context, real-time PCR assays (qPCR) were developed to detect SLEV and MAYV viruses. To standardize the qPCR, a plasmid containing the sequences of these viruses was used and, from these sequences, primers and probes were designed. For the MAYV assay it was possible to obtain 96.39% efficiency and, with regard to sensitivity, detection of one single viral RNA copy. As for the SLEV assay, an efficiency of 104.67% and sensitivity for detecting one single viral RNA copy were obtained. Regarding specificity, both assays were specific for the virus of interest. The standardized assays for SLEV and MAYV showed efficiency, specificity and sensitivity within the standards determined for qPCR, being suitable for use in routine laboratory diagnostics.
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Tesis |
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1
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VALDIR GOMES NETO
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Characterization of Ricinus communis L. genes associated with tolerance to abiotic stresses
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Líder : RENATO DELMONDEZ DE CASTRO
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MIEMBROS DE LA BANCA :
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RENATO DELMONDEZ DE CASTRO
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LIV SOARES SEVERINO
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LUIZ EDUARDO VIEIRA DEL BEM
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MANOEL TEIXEIRA SOUZA JÚNIOR
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MARCIO ALVES-FERREIRA
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Data: 30-abr-2021
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Resumen Espectáculo
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Castor bean (Ricinus communis L.) is an oilseed species globally recognized for an uncounted number of industrial applications and high market value of the oil extracted from its seeds. Besides, it has significant socioeconomic importance concerning its production being traditional and mostly carried out in Brazil by family farmers in the region of the Brazilian Northeastern semiarid region, where rudimentary crops predominate under adverse conditions typical of the region. Therefore, the present study aimed the characterization and overexpression of Castor bean genes associated with better tolerance to abiotic stresses during germination and initial seedling development. Three families of genes related to superoxide dismutase (SOD), small heat shock proteins (sHSP), and the nuclear transcription factor Y subunit B (NF-YB) were analyzed, according to the importance of these genes in the literature, and their selection by complementary analysis of within a Castor bean microarray for genes expressed under heat stress. The identification and characterization of these families in Castor bean was compared with angiosperm genomes, obtaining the profile of gene expression under conditions of abiotic stresses during imbibition, radicle protrusion (germination per se) and post-germination (young seedlings), and functional characterization of the target genes for tolerance to abiotic stresses through overexpression in Arabidopsis thaliana. In CHAPTER 1 (published article), we identified the SOD gene family in Castor bean (RcSOD), and the orthologous genes in angiosperms, showing the expression profile of RcSOD genes in embryos during imbibition and germination under different osmotic potentials (water restriction). The RcCuZnSOD1 and RcFeSOD8 genes were induced during imbibition by osmotic stress that induces ‘priming’ in Castor bean seeds as being associated with beneficial responses to seeds and seedlings vigor in different Castor bean genotypes. In CHAPTER 2 (article to be submitted), we identified possible regulatory elements of RcSOD genes under abiotic stresses and regulation by ABA, and the mechanism of regulation by the microRNA 398 for the RcCCuSOD4 and RcCuZnSOD3 genes. We identified the RcCuZnSOD1 and RcFeSOD8 genes as responsive to heat stress (35 °C) during imbibition and germination. Besides, we identified that other RcSOD genes (RcCCuSOD4, RcFeSOD7, RcCuZnSOD3) were induced in seeds under heat stress during radicle protrusion and in seedlings with 2 cm roots. It was possible to observe the subcellular localization of the RcSOD genes in Nicotiana benthamiana leaves, in which RcSOD genes (RcMnSOD5 and RcFeSOD8) may have differences compared to the subcellular location in A. thaliana. In CHAPTER 3 (published article), we initially identified 41 genes of the heat shock protein family predicted in Castor bean (RcsHSP), showing that tandem duplication seems to be one of the possible causes for the largest number of castor genes compared to A. thaliana. Besides, we showed the pattern of gene expression of 10 RcsHSP genes induced during radicle protrusion and early seedling stage. However, demonstrating the specificity of expression in roots, cotyledons, and leaves at different stages of seedling development under heat stress. Finally, we performed the functional characterization through the overexpression of two RcsHSP genes (RcsHSP12 and RcsHSP19) in A. thaliana, resulting in (a) higher percentage of seed germination under heat, osmotic and saline stresses; (b) greater enzymatic antioxidant potential of SOD; (c) and higher concentration of protective carbohydrates (sucrose and raffinose). In CHAPTER 4 (submitted article), we identified the family of genes of the nuclear transcription factor subunit B (NF-YB) in Castor bean and made the functional characterization of the RcNF-YB8 gene regarding the induction of early flowering in A. thaliana. The phylogenetic comparison allowed us to identify orthologous genes in angiosperms and motif patterns that may be associated with differences between the RcNF-YB subfamilies. The induction of RcNF-YB genes was observed to be greater during imbibition and germination compared to the post-germinative phase, also showing different profiles of induction and suppression by heat stress. The RcNF-YB8 gene demonstrated suppression by heat stress and a pattern of greater expression in leaves, while the overexpression in A. thaliana also demonstrated to induce early flowering, therefore, impacting the size of the plant and fruits, and consequent productivity. The results involve a broad characterization of genes from three important families in the response of seeds and seedlings to abiotic stresses and subsequent plant development, in which the characterization of the genes showed significant relevance indicating target genes potentially useful in breeding programs towards varieties of Castor bean with superior vigor aimed at better stand stablishment and crop development, and consequently better productivity of Castor bean by the family farmers in the Brazilian Northeast semiarid.
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2
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IGOR CARVALHO FONTES SAMPAIO
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EXOPOLISACARIDES PRODUCTION IN AEROBIC REACTOR THROUGH ENERGY INDUSTRY WASTES
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Líder : PAULO FERNANDO DE ALMEIDA
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MIEMBROS DE LA BANCA :
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PAULO FERNANDO DE ALMEIDA
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ANTONIO LUIZ BARBOSA PINHEIRO
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FABIO ALEXANDRE CHINALIA
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JOSILENE BORGES TORRES LIMA MATOS
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LANDULFO SILVEIRA JUNIOR
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PEDRO JORGE LOURO CRUGEIRA
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Data: 21-jun-2021
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Resumen Espectáculo
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The sustainable management of produced water (PW) poses a challenge to the oil industry. A biological method was evaluated for the treatment of this by-product in conditions of high salinity, through the production of the xanthan gum exopolysaccharide in culture containing produced water, sucrose, crude glycerin and constituents of mineral saline medium. This topic aims to solve the limitations of produced water use and contributes to its reuse for the production of an product used in the oil extraction chain. Xanthan gum production was achieved until the concentration of 25% of PW in the production medium. In a stirred tank reactor the batch with dialysed produced water (DPW) produced 25% more xanthan gum (p <0.01) than the control with distilled water (dW). Energy dispersion X-ray spectroscopy and Scanning Electron Microscopy demonstrated the incorporation of salts and metals from PW into xanthan gum. Analysis by Raman Spectroscopy showed that the exopolysaccharide has a chemical profile similar with xanthan gum and its composition was influenced by PW concentration, specifically regarding the carbohydrates content as well as pyruvate. Rheologically, it was observed that with up to 10% PW, solutions with total viscosity similar to the control produced with dW were obtained. The developed technology makes it possible to recycle PW and crude glycerin to generate xanthan gum as well as to reduce the consumption of drinkable water in the biopolymers industry. The association of alkaline stress with the surfactant Triton X-100 increased the production and quality of xanthan gum, with maximum values of 88.72% of production and 190.35% of viscosity in the medium prepared with DPW (P < 0.0001). The technology developed “surfactant/alkali stress” is an innovative way of reducing production costs and increasing the quality of EPS xanthan gum.
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3
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JORGE LUIS NICOLETI
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Determination of Corynebacterium pseudotuberculosis infection biomarkers in goats: Classical Methods, Nuclear Magnetic Ressonance and Chemometry
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Líder : RICARDO WAGNER DIAS PORTELA
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MIEMBROS DE LA BANCA :
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MARTINS DIAS DE CERQUEIRA
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MATEUS MATIUZZI DA COSTA
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RICARDO WAGNER DIAS PORTELA
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SILVANA BEUTINGER MARCHIORO
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VASCO ARISTON DE CARVALHO AZEVEDO
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Data: 30-jun-2021
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Resumen Espectáculo
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Caseous lymphadenitis (CL), an infectious disease caused by the Gram-positive bacterium Corynebacterium pseudotuberculosis in goats and sheep, is highly prevalent in small ruminant herds worldwide. Several economic losses, represented by the decrease in the value of leather, condemnation of carcasses and reproductive failures, have already been associated with the occurrence of the disease. Currently available techniques for clinical and laboratory diagnosis of the disease lack market availability and/or sensitivity and, therefore, infected animals can remain in the herd, serving as a source of infection for other animals. Nuclear Magnetic Resonance (NMR) and chemometrics are techniques that can identify metabolites not detectable by classical laboratory diagnostic techniques. The present study aimed to determine markers of C. pseudotuberculosis infection in goats using classical methods (clinical, hematological, biochemical), serological, NMR and chemometry. A herd of 173 Canindé goats was used, from which blood and serum samples were collected for analysis. The animals were classified as uninfected control (C), asymptomatic (A) and symptomatic (S), according to microbiological isolation, based on the content of caseous lesions and serological assay (ELISA). Complete blood counts, clinical biochemistry tests were performed to determine the concentration of serum compounds; 1H NMR analyzes NOESY (Nuclear Overhauser Effect SpectrosopY) and CPMG (Carr-Purcell Meiboom-Gill) and Partial Least Square Discriminant Analysis chemometry (PLS-DA) for verification and possible biomarkers for the disease. A high dissemination of the infection by C. pseudotuberculosis was observed in the herd, with 86.13% of positive animals, being 74.57% asymptomatic and 11.56% symptomatic. In the hemogram and clinical biochemistry analyses, the only statistical difference found was the higher level of serum urea in asymptomatic individuals than in non-infected animals. In the evaluation of 62 serum samples by NMR, the techniques employed were satisfactory in discriminating the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for CL. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, opening up promising possibilities for the establishment of NMR and chemometric techniques as CL suitable diagnosis methods, both because of the advantages of the techniques and the results reported herein for the first time in goats.
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4
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RODRIGO DA CRUZ FERREIRA
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Melipona quadrifasciata anthidioides, geoprópolis, multi-elements, IOES, HPLC.
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Líder : LIDERCIA CAVALCANTI RIBEIRO CERQUEIRA E SILVA
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MIEMBROS DE LA BANCA :
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LIDERCIA CAVALCANTI RIBEIRO CERQUEIRA E SILVA
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ADEMIR EVANGELISTA DO VALE
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MILLENO DANTAS MOTA
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MARIA DA PUREZA SPINOLA MIRANDA
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HUGO NEVES BRANDAO
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Data: 30-jul-2021
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Resumen Espectáculo
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In order to characterize the gross geopropolis of Melipona quadrifasciata anthidioides, and to evaluate the physical stability of a semi-solid cream-type formulation incorporated into the assets of this geopropolis. Samples of this product were provided by the meliponary of the Rio Seco Agroecology Center (CEARIS), located in the city of Amélia Rodrigues-BA (UEFS), (latitude 12 ° 23'08 '' S / longitude 38 ° 47'51 '' W. The experiments were carried out in the laboratories: (LAEX-UEFS), (LABIV – UEFS), (IGEO UFBA), (LABMICRO-UFBA) (LAMABI / ICS / UFBA), (LAPEMEC) UFBA). Initially, a patent prospecting with the term "propolis" and "geopropolis" was carried out. Subsequently, the microbiota present in the geopropolis was verified in addition to the quantification of macro and microelements of the raw material through IOES. The extracts used were prepared using the Sohxlet technique using 5g of geopropolis diluted in 50ml with alcohol at 96 ° GL, then an analysis of the chemical profile was performed using the HPLC technique in addition to the evaluation of its antioxidant activities and antimicrobial against gram negative E.coli and Gram positive Staphylococcus aureus. The elaborated formulation was subjected to accelerated stability tests recommended by ANVISA. In vitro spread tests were also applied in order to outline the quality performance of a product with potential for topical application. Patent prospecting showed only 3 deposits with the term “geopropolis”. The results of the microbiota of the geoprópolis in natura demonstrated that the quantitative values of microorganisms indicating the microbiological criteria used for food were high for Bacillus cereus, aerobic mesophilic bacteria and molds and yeasts. With the absence of Salmonella sp. and Clostridium sulfite reducer. Macro and microelements (mg/ kg) were quantified (Al 320414,40-36911,1) (As <LD -4,37) (Ba 38,36-211,11) (Cd <LD) (Co <LD- 14,12) (Cr 17,41-38,07) (Cu 10,63-28,73) (Fe 21973,96- 11536,47) (Mn 50,51- 310,51) (Mo <LO) ( Ni 2,28-21,74) (Pb 3,45-8,55) (Sb <LD-1,64) (Se <LD-1,01) (Si <LD- 150,77) ( Sn 4,92-16,14) (Sr 9,21-36,29) (V 28,77-78,73) (Zn 24,34-50,31) (Ca 672,38-94527) (K 1974,38-9198,91) (Mg 1961,17-7481,79) (Na 154,55-340,46) (P 16,59-51,07) beyond C (2,26-7,69%). These extracts showed phenolic compounds and flavonoids in addition to exhibiting high antioxidant activity. The analysis of the chemical profile allowed to identify: gallic acid, naringenin, kampferol and kaempferide. The product formulated with (2.5%) showed the best stability under the stress conditions tested. Its ability to incorporate and accept the geopropolis ethanol extract was significant, keeping the characteristics of the stability tests unchanged.
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5
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TATIANA OLIVEIRA DO VALE
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Application of the cultivation of microorganisms in the development of biotechnological strategies for the oil and gas industry
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Líder : FABIO ALEXANDRE CHINALIA
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MIEMBROS DE LA BANCA :
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Eloisa Pozzi
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FABIO ALEXANDRE CHINALIA
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ICARO THIAGO ANDRADE MOREIRA
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JOSILENE BORGES TORRES LIMA MATOS
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PAULO FERNANDO DE ALMEIDA
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Data: 10-ago-2021
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Resumen Espectáculo
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This work aims to innovate in the development of biotechnological strategies based on the cultivation of microorganisms and to mitigate the main challenges of the oil and gas industry. Oil is a high-demand commodity globally. With the economic development of countries there is a need to increase their production and respond quickly to the challenges faced by the sector. Biotechnology can offer the technological contribution in bioprocesses that meet these adversities. Currently, the greatest burdens are concentrated in the processes of increasing oil recovery, destination of the water produced and control of souring. In addition, during operational protocols, spill accidents may occur, so it is important to establish strategies for impacted areas. In this way, microorganisms can be applied widely and effectively in different scenarios and their metabolic diversity allows the production of bioactive substances. In the present study, strains were isolated for the production of biosurfactants that improve oil recovery in mature wells, in addition to being applied in the bioremediation of affected environments. The process becomes more attractive with the production of these molecules in water produced with a high salt content and without additional treatments. Sulfate Reducing Bacteria (SRB) were used in the water produced, used in a new rapid monitoring strategy. Therefore, the objective of this study is to generate environmentally sustainable and economically competitive solutions from the cultivation of microorganisms in order to reduce costs and minimize impacts on the environment.
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6
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FILIPE SAMPAIO REIS DA SILVA
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IMPLEMENTATION OF SYNTHETIC BIOLOGY PRINCIPLES FOR THE CONSTRUCTION OF ADVANCED GENETIC SYSTEMS FOR BIOTECHNOLOGY
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Líder : LUIS GUSTAVO CARVALHO PACHECO
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MIEMBROS DE LA BANCA :
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Rommel Thiago Juca Ramos
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LUIS GUSTAVO CARVALHO PACHECO
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SILVANA BEUTINGER MARCHIORO
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THIAGO LUIZ DE PAULA CASTRO
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VASCO ARISTON DE CARVALHO AZEVEDO
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Data: 10-sep-2021
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Resumen Espectáculo
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Synthetic biology was defined as a specific area of knowledge in the 21st century and has already been introduced in the fields of industry, energy, agriculture and health. In its earliest days, synthetic biology was consolidated as a separate discipline from molecular biology, focusing on the engineering of new biological functions by incorporating computer sciences and electrical engineering concepts. The construction of synthetic genetic circuits with complex functionalities was the ammunition for the progress of this novel area. More recently, the CRISPR revolution in genetic engineering helped establish multiple alternative molecular tests with potential application, for example, at the point of patient care or in industrial quality control. This work was based on the hypothesis that it is possible to apply synthetic biology principles to the idealization and optimization of genetic tools that are already commonly used for biotechnological purposes, including recombinant protein expression systems and genetic biosensor devices. First, a genetic circuit was constructed to aid the expression of soluble recombinant proteins through controlled intracellular processing. This system yielded a high concentration of soluble recombinant protein without the solubilization tag was up to 67.3%. The application of this system will facilitate the production of proteins of difficult expression and of biotechnological interest, such as Bst-LF polymerase, which has application in biosensor systems. The objective is to integrate this enzyme into a diagnostic tool also developed in the context of this study, based on the CRISPR technology, which has already been adapted to detect the RNA of the SARS-CoV-2 virus. The test had an analytical sensitivity of 50 viral copies/μL and showed high accuracy in patient samples (area under the ROC curve = 1.0; CI: 0.715 - 1.00), using detection by a mobile phone application with computer vision integrated. The design of both technologies demonstrates the potential of synthetic biology for the development of biotech products and tools.
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