Banca de DEFESA: MARCELA ALMEIDA MUHANA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARCELA ALMEIDA MUHANA
DATA : 11/09/2020
HORA: 09:00
LOCAL: on line
TÍTULO:

Evaluation of the accuracy of the Bahiafarma and Bio-manguinhos rapid tests for the diagnosis of arboviruses: dengue, Zika and chikungunya

PALAVRAS-CHAVES:

Rapid diagnostic test, dengue, Zika, Chikungunya

PÁGINAS: 40
GRANDE ÁREA: Ciências da Saúde
ÁREA: Saúde Coletiva
RESUMO:

Since 2015 Brazil has been experiencing the simultaneous circulation of arboviruses dengue, Zika and chikungunya. In this way, it is essential to use tools that are sensitive, specific and timely to detect such infections and assist health professionals regarding treatment. The aim of this study was to assess the accuracy of the rapid diagnostic tests (TRD) of dengue, Zika and chikungunya of two companies that obtained registrations at Anvisa / Brazil: Bahiafarma (BF) and BioManguinho (BM). Serum samples collected during the acute phase and paired convalescent samples from a surveillance study sample for RT-PCR detection of arbovirus infections among patients with acute febrile illness in a health unit in Salvador-Bahia. As controls, samples of serum from patients with acute febrile illnesses identified by the same study of surveillance and with a result of negative RT-PCR for the three arboviruses and also samples of blood donors. To estimate the number of samples in the evaluation, it was considered an expected sensitivity and specificity for tests of 90%, an accuracy of 8% and with 95% confidence, with the need for 60 acute samples and 60 convalescent for each group of cases and controls. The final sample included 54 cases of dengue (53 acute samples and 41 convalescent), 12 cases of Zika (11 acute samples and 7 convalescent) and 60 cases of Chikungunya (60 acute samples and 60 convalescent) and two control groups: 60 arbovirus-negative febrile patients and 23 donors of blood. The sensitivity and specificity of the dengue, Zika and chikungunya of BF and BM and we present in the summary the IgM result of the sample convalescent considering that the tests assess the presence of antibodies. To the convalescent samples of dengue, chikungunya and Zika from Bahiafarma presented, respectively, a sensitivity of 51.2% (95% CI 32.9-60.9%), 56.7% (95% CI 44.1-68.4%) and 14.3% (95% CI 2.5-51.3%) and a specificity of 95.2% (95% CI 90.0-97.8%), 96.3% (95% CI 90.8-98.5%) and 96.3% (92.0-98.3%). The sensitivities of the BM TRD in convalescent samples were: dengue-60.0% (95% CI 44.6-73.7%), chikungunya-98.3% (95% CI 91.2-99.7%), Zika-100% (95% CI 64.6-100%) and the specificities were, respectively, 65.5% (95% CI 56.3-73.6%), 93.4% (95% CI 87.0- 96.8%) and 68.8% (95% CI 61.1-75.6%). Therefore, we recommend only using the 10 rapid bio-cuff chikungunya test, after the 10th day of symptom onset. The other tests had insufficient performance.

MEMBROS DA BANCA:
Externo ao Programa - 090.842.665-87 - GUBIO SOARES CAMPOS - UAM
Presidente - 1369353 - GUILHERME DE SOUSA RIBEIRO
Externo à Instituição - MOYRA MACHADO PORTILHO