Banca de DEFESA: ICARO SANTOS LOPES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ICARO SANTOS LOPES
DATA : 22/02/2021
HORA: 14:00
LOCAL: RNP webconferência
TÍTULO:

Identification and classification of CRISPR arrays found in Xanthomonas

PALAVRAS-CHAVES:

CRISPR, Xanthomonas, bioinformatics, phytopathogenic

PÁGINAS: 46
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
SUBÁREA: Biologia e Fisiologia dos Microorganismos
ESPECIALIDADE: Bacterologia
RESUMO:

One of the decade’s most important discoveries in biotechnology is a defense mechanism found in prokaryotes that concedes adaptative immunity to its owner. This system called CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) consists in genomic integration of some sequences from bacteriophages, creating cellular “memory”. At this point, there are few studies about CRISPR’s role in prokaryotes’ interactions with the environment and organisms other than viruses. There is also little knowledge about how bacteria acquire this mechanism and its prevalence in species of economical interest, like phytopathogens. With this work, we intend to identify and classify CRISPR sequences found in the Xanthomonas genus, describing how this system affects interactions between the bacteria, their host plants and environmental viruses. In addition, we verify the hypothesis concerning these sequences’ origins in each Xanthomonas species, considering environmental influences and inheritance from a common ancestor. 239 complete Xanthomonas genomes were analyzed and CRISPR sequences were found in 102 of them. The final results imply that both environmental conditions and the common ancestor play a part to determine the presence, absence and characterization of CRISPR sequences in Xanthomonas. Therefore, it’s possible that both hypotheses are partially correct. Vast diversity observed in the spacers across the genomes indicates an intense network of environmental interactions to which bacteria are subordinated in order to carry out their phytopathogenic role.

MEMBROS DA BANCA:
Presidente - 1676978 - MILTON RICARDO DE ABREU ROQUE
Interno(a) - 2356247 - PEDRO MILET MEIRELLES
Interno(a) - 2415395 - THIAGO LUIZ DE PAULA CASTRO
Externo(a) à Instituição - BRENA MOTA MOITINHO SANT'ANNA - UFBA
Externo(a) à Instituição - VIVIANE GRAZIELLE DA SILVA - UFMG