Banca de DEFESA: TIAGO SOUSA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : TIAGO SOUSA DA SILVA
DATA : 12/11/2020
HORA: 09:00
LOCAL: sala virtual (plataforma Conferência Web RNP)
TÍTULO:

Molecular characterization of Mycobacterium tuberculosis complex strains isolated from a reference center for tuberculosis research

PALAVRAS-CHAVES:

Tuberculosis; Bacterial resistance; Genotyping

PÁGINAS: 56
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
ESPECIALIDADE: Microbiologia Médica
RESUMO:

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis Complex (CMTB) mycobacteria, a disease that primarily affects the lungs. The use of tools based on molecular biology has allowed advances in the field of human, animal and environmental medicine, bringing many benefits in the diagnosis of infectious diseases, significantly reducing the time from diagnosis to the institution of treatment combined with this advance, techniques such as the spoligotyping associated with classical epidemiology has enabled the identification of transmission chains and can be used to assess the efficiency of disease control programs in different parts of the world. This study aims to investigate the genetic diversity of MTB complex strains isolated from patients at a reference center for tuberculosis investigation. This study included bacterial isolates belonging to the MTB complex, originating from sputum samples sent to the Bacteriology Laboratory of the Jose Silveira Foundation for mycobacteriological diagnosis, with decontamination by the NALC-NaOH method, during the period from 2016 to 2018. The isolates were genotyped by spoligotyping technique and the results were analyzed visually and entered in binary format and compared with the standards described in the SITVIT database in order to identify the corresponding Spoligo International Type (SIT). The 46 samples with complete genetic profile in hybridization were classified into 26 SIT. The LAM line was the most frequent 43.1% (22/51). Regarding the antimicrobial sensitivity of the 150 isolates genotyped as M.tuberculosis, 143/150 (95.3%) showed sensitivity in vitro to all drugs tested while 7/150 (4.7%) showed resistance to at least one anti-drug -ALSO. Genotyping helped in the discovery of epidemiologically related cases, it was possible to observe three regions with a higher concentration of cases in the neighborhoods. We believe that knowledge about the characteristics of these patients will contribute to the development of policies aimed at improving the diagnosis and treatment of patients. Therefore, this study will greatly contribute to the provision of laboratory indicators for the control of tuberculosis and will serve in decision making by Brazilian municipalities.

MEMBROS DA BANCA:
Presidente - 1675209 - MELISSA HANZEN PINNA VALENTIM
Interno - 1187203 - JOICE NEVES REIS PEDREIRA
Externo ao Programa - 2371222 - SORAIA MACHADO CORDEIRO