Banca de DEFESA: LUCAS DE ALMEIDA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCAS DE ALMEIDA SILVA
DATE: 25/08/2023
TIME: 09:00
LOCAL: Instituto de Biologia
TITLE:

CHARACTERIZATION OF Acinetobacter baumannii ISOLATED FROM PATIENTS WITH BLOODSTREAM INFECTIONS IN SALVADOR, BAHIA

KEY WORDS:

Acinetobacter baumannii, multiresistance, carbapenems, resistance genes, genotyping

PAGES: 88
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
SPECIALTY: Microbiologia Médica
SUMMARY:

Acinetobacter baumannii is a gram-negative, aerobic, immobile coccobacillus bacterium. A. baumannii is an opportunistic pathogen of great clinical concern and belongs to the acronym “ESKAPE”, a group of multidrug-resistant pathogens, responsible for most outbreaks of nosocomial infections, including bacteremia, pneumonia, and septicemia in patients with critical immune status and hospitalized in Intensive Care Units (ICUs). A. baumannii has the ability to acquire different mechanisms of resistance to the most varied antibiotics, on a large scale the β-lactams. It is very important to know the pathogen, as well as the local epidemiology so that epidemiological control measures are properly implemented. Therefore, the objective is to describe the phenotypic and genotypic profile of A. baumannii isolates from patients with bloodstream infections, from two hospitals located in the city of Salvador, BA. Identification was determined by an automated system, antimicrobial susceptibility testing and _ECIM were performed using disk diffusion and the results were interpreted based on CLSI guidelines. Genes were detected by PCR technique to investigate resistance genes, and molecular typing was performed by ERIC-PCR technique. Thirty-six samples from patients with bloodstream infections identified in the period from 2015 to 2019 were evaluated; among the 36 isolates, 50.0% were resistant to carbapenems (imipenem and meropenem), with 44% of the isolates being MDR and one isolate was XDR. For the _ECIM test, 50.0% of the isolates were positive for metallo-β-lactamases. Investigation of resistance genes by PCR detected genes _blaOXA-23 (15, 41.6%), _blaOXA-24 (1, 2.7%), _blaCTX-M (3, 8.3%), _blaKPC (7, 19.4%) and _blaVIM (18, 50.0%). In three CarbR cases, it was not possible to clarify the resistance mechanism. Molecular typing using ERIC-PCR showed similarity in two large groups, with 5 clones in each cluster. Molecular typing by PFGE showed 31 distinct groups of clones and three major groups of A. baumannii resistant to VIM-producing carbapenems. The prevalence of multidrug-resistant A. baumannii isolates that produce carbapenemases, ESBLs, and metallo-β-lactamases detected in this study is of great concern and stands out with a high need for epidemiological and infection control measures.

COMMITTEE MEMBERS:
Presidente - 1187203 - JOICE NEVES REIS PEDREIRA
Interna - 2371222 - SORAIA MACHADO CORDEIRO
Externo à Instituição - HERMES PEDREIRA DA SILVA FILHO - UFRB