Banca de DEFESA: FERNANDA COSTA DA SILVA MACIEL

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : FERNANDA COSTA DA SILVA MACIEL
DATA : 08/05/2020
HORA: 14:00
LOCAL: PPEC
TÍTULO:

ANALYSIS OF HYDROCARBON DEGRADATION FROM PETROLEUM BY NATIVE MICRO-ORGANISMS IN AN AREA OF “ONSHORE” EXPLORATION, THROUGH TESTS OF COLUMN

PALAVRAS-CHAVES:

Bioremediation 1. Column tests 2. Hydrocarbons 3. Microbiological decay 4.

PÁGINAS: 147
GRANDE ÁREA: Engenharias
ÁREA: Engenharia Civil
RESUMO:

The complexity involved in the analysis of the flow of substances in granular substrates is attributed, in addition to the complexity inherent to each phase involved, to the various phenomena developed by the interaction of the percolating fluid with the soil particles. Obtaining transport parameters is essential to predict the progress of contamination and to assist in the management of contaminated areas. In this research, results of column tests are used to analyze the transport of the dissolved phase of a fluid, production water (AP), with a high content of petroleum hydrocarbons in a sandy-clay soil, called várzea soil, coming from the surroundings. of an onshore oil exploration area. Among the processes established during the percolation of the fluid under study, reductions in permeability were detected, as a consequence of the occurrence of the clay dispersion phenomenon. The results of the column tests showed decays in the concentrations of total petroleum hydrocarbons (TPH) of the collected fluids, which required in the modeling of the arrival curves the use of a non-linear decay term (m), which was associated with the occurrence degrading activity of hydrocarbons by microorganisms native to the soil. To certify the existence of TPH degrading microbiota, a set of microbiological tests, with the application of traditional laboratory techniques and genetic analyzes, were used in soil samples taken from the columns at different times. Microorganisms cultured using traditional techniques (plate culture with culture medium) were isolated and subjected to oxidation tests with the DCPIP indicator for the selection of fungi and bacteria with the potential to degrade hydrocarbons. The selected specimens had their genomes sequenced (Sanger Sequencing) and could be identified. Species of bacteria (belonging to the genera Bacillus and Lysinibacillus) and fungi (genera Malassezia and Talaromyces) were detected, not yet associated in the literature with the degrading activity of TPH. Metagenomic analyzes performed on soil samples revealed that the highest percentages of the soil bacterial community were associated with HTP consumption, according to literature data. All microbiological tests, performed after column tests, pointed out the predominance of bacteria in relation to fungi. The confirmation of the existence of native microorganisms with potential for hydrocarbon degradation in the analyzed várzea soil samples, opens the possibility of developing more appropriate and safer in situ biological remediation techniques for the area. In addition, the incorporation of these organisms to low permeability soils can enable the system of mineral / biological containment barriers, in addition to the retention of the contaminant, its degradation. 

MEMBROS DA BANCA:
Externo à Instituição - ANDRÉ LUÍS BRASIL CAVALCANTE
Presidente - 6285989 - ANTONIO FERNANDO DE SOUZA QUEIROZ
Interno - 1299309 - MIRIAM DE FATIMA CARVALHO MACHADO
Interno - 1219972 - SANDRO LEMOS MACHADO