Banca de DEFESA: STEVE BIKO MENEZES HORA ALVES RIBEIRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : STEVE BIKO MENEZES HORA ALVES RIBEIRO
DATA : 26/02/2021
HORA: 08:00
LOCAL: https://conferenciaweb.rnp.br/conference/rooms/joana-paixao-monteiro-cunha/invite
TÍTULO:

Immunogenic characterization of the antigen group (gag) gene of strains of the human immunodeficiency virus type 1 (HIV-1) circulating in Brazil.

PALAVRAS-CHAVES:

HIV-1, gag, epítopes, Brazil.

PÁGINAS: 148
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Biologia Molecular
RESUMO:

RIBEIRO. S B M H A. Immunogenic characterization of the antigen group (gag) gene of strains of the human immunodeficiency virus type 1 (HIV-1) circulating in Brazil, 2020. 105. Exame de Qualificação – Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, 2020. The great HIV genetic variability represents a major obstacle both to infection control by the host's immune system and to the development of efficient drugs and vaccines. CTL cells that recognize epitopes within the HIV antigen group (gag) gene have been associated with early infection control and used in HIV vaccine development approaches. Having that said, the objective of this study was to investigate the variability of HLA class I binding epitopes in the gag gene of HIV-1 strains circulating in Brazil, available in a publicly accessible database. Therefore, the allele variability and predominance of HLA class I in the Brazilian population was determined so that the identification of epitopes of the gag gene of HIV-1 strains circulating in Brazil. For the allele predominance of the HLA, the Allele Frequency Net Database (AFND) was used, as gag regions were removed from through the electronic address https://www.hiv.lanl.gov, with the defined genes (gag) and the alleles most prevalent in the Brazilian population. The Immune Epitope Database (IEDB), using the link tool, was used to determine which are the best Brazilian HLA-linked epitopes. Modeling the protein structure for the antigen with the best identity value was also carried out using the SwissModel program. From this research, a table was generated with the description of the epitopes present in the most circulating subtypes in Brazil according to each previously determined class I HLA allele (HLA-A * 2, A * 24, A * 03, A * 01, A * 68, B * 35, B * 44, B * 15, B * 51 and B * 07), HXB2 and subtypes B, F, and C of HIV-1 and the position of the epitope in relation to the gag gene. As a result, 1320 total epitopes were found, 316 for HXB2, 336, 321, and 302 for subtypes B, F1, and C respectively. From this preliminary analysis, 24 highly conserved candidate epitopes were found between subtypes B, F1 and C. It is important to note that one of the epitopes identified in this study - EPRGSDIAGTTSTL - has not been described in a patent database and in the academic literature until that date. Thus, the variability of HLA class I binding epitopes in the gag gene found in the present study generated data that characterize and describe circulating epitopes in Brazil, contributing to the understanding of the convergence of these epitopes in the world and to the field of research and vaccine development. This study will contribute effectively to assessing the subtype-specific variability of HLA allele-binding epitopes in the genomes of different circulating HIV-1 subtypes and recombinants in Brazil.

MEMBROS DA BANCA:
Interno(a) - 1854449 - ALINE CRISTINA ANDRADE MOTA MIRANDA MASCARENHAS
Externo(a) à Instituição - ERIC ROBERTO GUIMARAES ROCHA AGUIAR
Presidente - 2766939 - JOANA PAIXAO MONTEIRO CUNHA