Banca de DEFESA: VICTOR DE BARROS SERRANO NEVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : VICTOR DE BARROS SERRANO NEVES
DATA : 04/08/2020
HORA: 14:00
LOCAL: UFBA
TÍTULO:

microRNA AS BIOMARKERS FOR THE IDENTIFICATION OF INDIVIDUALS WITH PRE-DIABETES AND EARLY DIAGNOSIS OF DIABETES.

PALAVRAS-CHAVES:

Diabetes; Molecular Mechanism; Gene Expression; Transcriptome

PÁGINAS: 63
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Biologia Molecular
RESUMO:

Introduction: Diabetes Mellitus (DM) is a chronic disease developed by defects in insulin signaling, secretion or production. Prediabetes is a asymptomatic state in which subjects fasting glucose is between 100-120mg/dl and glycated hemoglobin is at the range of 5,7-6,5%. Defined before as a latent state it is now known that is a risk factor for nephro and neuropathy development and also increase in β-cell destruction. In this context, becomes necessary the identification of biological markers that presents a disrupted expression due to the pathological state thus contributing to an early diagnostic. miRNAs are non-coding RNA considered important in the pos-translation regulation of genes. They are pointed out as potential biomarkers, since their expression level becomes altered due to the development or aggravation of many diseases such as diabetes, cancer and schizophrenia. The present study aimed to identify an expression microRNA (miRNA) profile capable of confer early prediabetes diagnostic. Publicly available transcriptome was analyzed through bioinformatic tools. Results: After screening conducted at NCBI GEO datasets, one transcriptome matched our inclusion criteria. From which thirty-three miRNA were significatively upregulated in prediabetic subjects versus control (FC>1,5 p-valor≤ 0,05) and two were upregulated in the diabetix cersus control group (FC>1,5 p-valor≤ 0,05) A miRNA-mRNA network was created using the upregulated miRNA and its regulated targets in the validation transcriptome using the Cytoscaped software. Five miRNAs presented a greater centrality score from the miRNA-mRNA network. In which two (miR-93 and Let-7a) had better values for sensibility and specificity (AUC =0,829 e 0,824 respectively). Conclusion: The five miRNA identified are involved in several intracellular pathways related to insulin resistance and destruction of β-pancreatic cells and are differentially modulated in the transcriptome analyzed in this study, which can be identified as promising exploratory biomarkers for the early diagnosis of DM2.

MEMBROS DA BANCA:
Externo à Instituição - CAROLINA KYMIE VASQUES NONAKA
Externo à Instituição - GLÓRIA REGINA FRANCO - UFMG
Interno - 1278694 - SIMONE GARCIA MACAMBIRA