Banca de DEFESA: RAFAEL SOUZA DE ALMEIDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAFAEL SOUZA DE ALMEIDA
DATE: 14/07/2023
TIME: 14:00
LOCAL: UFBA
TITLE:

IN SILICO ANALYSIS OF MICRORNAS AND THE CORRELATION WITH PATHWAYS INTRACELLULAR ASSOCIATES WITH ULCER HEALING CUTANEOUS: A PROJECTION TO SICKLE ULCER.

KEY WORDS:

Sickle Cell Anemia; Ulcers; Healing; miRNAs

PAGES: 75
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

Introduction: Sickle cell anemia is a chronic, hereditary, autosomal recessive hematological disorder. In Brazil, sickle cell anemia is a social and public health problem. Sickle cell ulcers are among the most serious complications of sickle cell anemia (SCA). Until recently, treatment strategies for sickle cell ulcers were limited to wound bed preparation, removal of necrotic tissue, and infection control. The investigation of biomarkers to assess the degree of ulcer healing appears as an interesting therapy for controlling the condition. Objective: To study the miRNA expression profile and its correlation with intracellular pathways associated with the development and healing of skin ulcers. Methodology: Public data of transcriptomes, GEO access code of GSE121996 were analyzed. The samples were examined using bioinformatics tools through the Galaxy platform. Subsequently, a filtering was performed based on the ratio of the “fold-change” expression level and the adjusted p-value <0.05, in order to identify the differentially expressed microRNAs comparing the moments: 0h vs 24hr, 0h vs 5 days and 24hr vs 5 days. Results: We identified 56, 82 and 46 differentially expressed miRNAs at 0h vs 24hr, 0h vs 5days and 24h vs 5 days, respectively. Among the identified microRNAs, Mir129, Mir155, Mir204 and Mir211, Mir34c, Mir298 and Mir224 stand out, which regulated genes involved in the stages of inflammation and cell proliferation in wound healing. Conclusion: Based on the results, it was possible to evidence a set of microRNAs differentially expressed throughout the healing process. Of these, Mir34c, Mir298 and Mir224 stand out, which in the literature are involved with cancers, but which in this work are associated with pathways of signaling associated with wound healing, aiding in the inflammatory response, migration of keratinocytes and fibroblast differentiation. The future perspective is to establish a correlation between these microRNAs and their target genes during wound healing of sickle cell ulcers in a biological system.

COMMITTEE MEMBERS:
Presidente - 1278694 - SIMONE GARCIA MACAMBIRA
Interna - 1854449 - ALINE CRISTINA ANDRADE MOTA MIRANDA MASCARENHAS
Externo à Instituição - LEONARDO PAIVA FARIAS - Fiocruz - PE