Banca de DEFESA: IASMIN SOUZA LIMA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : IASMIN SOUZA LIMA
DATE: 09/12/2021
TIME: 09:00
LOCAL: Instituto Multidisciplinar em Saúde, UFBA (remota)
TITLE:

Exploratory analysis of genomic scanning and association with Helicobacter pylori infection

KEY WORDS:

GWAS. Helicobacter pylori. Infection.

PAGES: 56
BIG AREA: Ciências Biológicas
AREA: Genética
SUBÁREA: Genética Molecular e de Microorganismos
SUMMARY:

Helicobacter pylori infection causes an inflammatory response in the host and the development of various gastric diseases, such as gastric cancer. Evidence in the literature indicates that susceptibility to infection by the bacteria is associated with the individual's genetic factors. Several studies demonstrate that genetic polymorphisms in genes related to immune response have a direct relationship with H. pylori infection. However, few Genome Wide Association Studies (GWAS) have been performed. So far, there are no data for the Brazilian population or for a cohort of children. Therefore, this work conducted a genomic scan analysis in a mixed Latin American population. For this purpose, 1,162 children participating in the Social Changes, Asthma and Allergy in Latin America (SCAALA) program were included in this work. DNA was extracted from peripheral blood samples using the commercial Qiagen Flexigene kit. Single nucleotide variants (SNVs) for the SCAALA population were genotyped using the commercial Illumina HumanOmni2.5-8 panel. Detection of H. pylori infection was performed using an enzyme-linked immunization assay (ELISA) following the manufacturer's instructions. Logistic regression analysis was used to examine the association with H. pylori infection, assuming an additive genetic model. As a result, we found 22 SNVs suggestively associated with H. pylori infection. Of this total, 2 polymorphisms stood out in our study. The first suggestive SNV was rs77955022 (OR = 2.27; CI (95%) = 1.65 - 3.13; p=4.83-07), which is located on chromosome 5 in an intronic region of the EXOC3 gene (exocyst complex component 3). The second SNV was rs10914996 (OR = 0.61; CI (95%) = 0.50 - 0.74; p=8.97-07), which can be found in the 1p35.1 region. Our data suggest that single nucleotide variants may condition the individual to Helicobacter pylori infection. Furthermore, our work, so far, is the only one that brings genomic scanning analyzes for H. pylori infection in a mixed and non-adult population. However, further studies should be carried out to better understand the functional effects of these variants, as well as to replicate these findings in other populations.

BANKING MEMBERS:
Presidente - 2316383 - CINTIA RODRIGUES MARQUES
Externo ao Programa - 2983099 - RYAN DOS SANTOS COSTA
Externo à Instituição - GUSTAVO NUNES DE OLIVEIRA COSTA - UNIFACS