Banca de DEFESA: LUISA CARREGOSA SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUISA CARREGOSA SANTOS
DATA : 03/12/2021
HORA: 14:00
LOCAL: IMS/CAT/UFBA
TÍTULO:

ANTIMETASTATIC EFFECT ON LUNG CANCER CELLS AND ANTIANGIOGENIC FROM PLA2 LYS49 MJTX-II, ISOLATED FROM Bothrops moojeni SNAKE VENOM

PALAVRAS-CHAVES:

PLA₂, snake venom, Bothrops moojeni, lung cancer, metastasis and angiogenesis

PÁGINAS: 85
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Química de Macromoléculas
ESPECIALIDADE: Proteínas
RESUMO:

Cancer is characterized by the uncontrolled division of cells, which escape the control mechanisms of the cell cycle and apoptosis, and invade other tissues, promoting angiogenesis and metastasis. Lung cancer is the third most common type of cancer in Brazil and is associated with high incidence and mortality rates, as a result of the aggressiveness sustained by the processes of angiogenesis and metastasis. In this context, several studies have shown that PLA₂ isolated from snake venom have been investigated with the aim of developing future drugs for cancer therapy. In this work we show for the first time the antimetastatic and antiangiogenic effect of MjTX-II, a PLA₂ isolated from Bothrops moojeni snake venom on lung cancer cells and endothelial cells. From in vitro cell culture assays and ex vivo techniques, we demonstrate that PLA₂ acts on A549 lung cancer tumor cells, reducing their viability and inhibiting processes that are fundamental to their growth and metastasis, such as adhesion, migration, invasion and decrease of actin cytoskeleton without significantly interfering with non-tumorigenic lung cells (BEAS-2B). Furthermore, it caused alterations in the cell cycle, led to the production of reactive oxygen species, as well as modulated the expression of pro and antiangiogenic genes and increased the expression of vascular endothelial growth factor (VEGF) in endothelial cells HUVECs. Finally, the toxin also inhibited ex vivo angiogenesis processes in aortic ring model. We conclude, therefore, that MjTX-II has an antimestatic and antiangiogenic effect in vitro and ex vivo, representing a biotechnological tool for the study of neoplasms and a promising molecule as a pharmacological model for antitumor therapy.

MEMBROS DA BANCA:
Presidente - 1071194 - DAIANA SILVA LOPES
Interno - 2315391 - FABRICIO FREIRE DE MELO
Externo à Instituição - ELIÉCER JIMÉNEZ CHARRIS