Banca de DEFESA: BEATRIZ ALMEIDA SAMPAIO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : BEATRIZ ALMEIDA SAMPAIO
DATA : 22/10/2021
HORA: 14:00
LOCAL: https://meet.google.com/mxn-pfxe-emf
TÍTULO:
Evaluation of the immunogenicity of recombinant Mycoplasma agalactiae proteins in goats for selection of vaccine candidates
PALAVRAS-CHAVES:
Contagious agalactia; M. agalactiae; recombinant; subunit; vaccine
PÁGINAS: 83
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
ESPECIALIDADE: Microbiologia Médica
RESUMO:
The contagious agalactia is a disease classically caused by Mycoplasma agalactiae affecting small ruminants worldwide being considered endemic in some countries. Characterized by causing mastitis, agalactia, arthritis and keratoconjunctivitis, the disease results in huge losses to small and large producers. Characterized by causing mastitis, agalactia, arthritis and keratoconjunctivitis, the disease results in great economic losses for small and large producers. As an alternative to the existing immunoprophylactic measures, the present study aimed to develop a recombinant subunit vaccine against M. agalactiae being evaluated for its specific immune response in goats. Twelve goats were used, divided into three groups (control, recombinant and bacterin). The animals in the recombinant group were immunized with a solution of recombinant proteins, these being two lipoproteins from M. agalactiae (P40 and MAG_1560), while the bacterin group was immunized with inactivated M. agalactiae (109 CFU/ml). Both solutions were emulsified in Freund's adjuvant. The animals were evaluated for a period of 181 days for the production of IgG antibodies and in vitro response of PBMC's to the stimulus of recombinant proteins with subsequent analysis of the expression of IL-1β, IFN-γ, IL-12 and MHC-II. The specific production of antibodies against total extract and membrane proteins of M. agalactiae was observed in the serum of animals immunized with recombinant proteins. Animals immunized with bacterin were also able to produce specific antibodies mainly against the MAG_1560 protein. The evaluation of the development of the immune response through the gene expression of cytokines showed an increase in mRNA of the proinflammatory cytokine IL-1β over time in response to the stimulation of P40 and MAG_1560 proteins by the recombinant and bacterin groups. In the bacterin group, a reduction in IFN-γ mRNA levels was observed, while in the recombinant group, in response to the P40 stimulus, there was an increase in expression, but none or a reduction in expression with MAG_1560 was observed. In the recombinant group, IL-12 cytokine expression in 168 days has increased expression with the stimulation of P40 when compared to non-stimulated cells and MAG_1560 showed increased expression with 56 days. Over time, the bacterin group had an increase in IL-12 gene expression. The proteins were able to stimulate MHC-II gene expression in both groups. The subunit vaccine model presented here has promising data for a vaccine capable of inducing cellular and humoral responses. The production of new vaccines from recombinant antigenic proteins will enable an alternative to existing vaccines (inactivated and attenuated), in a safe and effective way, for the prevention of contagious agalactia, contributing to animal health with an economic impact on increasing goat productivity
MEMBROS DA BANCA:
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo ao Programa - 2549111 - RICARDO EVANGELISTA FRAGA
Externa ao Programa - 1090486 - SILVANA BEUTINGER MARCHIORO