Banca de DEFESA: ANA MARQUES BOTELHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANA MARQUES BOTELHO
DATA : 18/12/2020
HORA: 14:00
LOCAL: Instituto Multidisciplinar em Saúde
TÍTULO:

Evaluation of galactagogue activity of ethanolic extract of Valeriana officinalis Linn and its dichloromethane fraction, in an experimental model with rats

PALAVRAS-CHAVES:

 

Breast milk, Wistar mouse, lactation, Valeriana, hypogalactia

PÁGINAS: 94
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
RESUMO:

Breast milk is a complete and suitable food for the vast majority of newborns, presenting several benefits for both mother and baby. However, some women have hypogalactia caused by situations such as premature birth, re-lactation, metabolic disorders and psychological problems related to stress. In this context, foods, medicines and medicinal plants with galactagogue properties are used to increase milk production. Valeriana (Valeriana officinalis L.) is a medicinal plant known for its sedative properties, which is why it is commonly used by lactating women as an alternative to treat anxiety states, common during breastfeeding. The objective of the present study was to evaluate the galactagogue activity of the ethanolic extract of V. officinalis (EEVO) and of the dichloromethane fraction (DF), in an experimental animal model. Lactating Wistar rats were treated for 17 days by means of intraperitoneal injection with 0.2 ml of distilled water, EEVO (12.5; 25, 50; 100 and 200 mg / kg) and DF (25 and 50 mg / kg). Daily and total weight gain of the litter, estimate of milk production, biochemical composition of milk, histological analysis of the mammary glands and concentration of prolactin and oxytocin were evaluated. The EEVO group (50 mg / kg) obtained the best results among the tested substances, with an increase in daily and total weight gain of the puppies (p <0.05), protein concentration (31.02%) and total triglycerides (27.94%) of milk (p <0.05), as well as higher concentrations of serum prolactin (43.41%) and in the mammary gland (25.85%) (p <0.05). There was a decrease in oxytocin levels both in the neurohypophysis and in the serum (p <0.05). It was also observed a decrease in alveolar epithelial cells in the involution phase (p <0.05), an increase in the number of lobules and blood vessels. DF (25 and 50 mg / kg) increased the pups' total weight gain (9 and 11.79%, respectively), proteins (77.88 and 89.32%, respectively) and total triglycerides (263.24 and 292, 02%, respectively). DF (50 mg / kg) increased the number of alveoli and lobes (p <0.05). Thus, the extract and fraction of dichloromethane from the root of Valeriana officinalis showed galactagogue activity, by increasing the weight gain of the litter as a reflection of the increased concentrations of proteins and triglycerides in milk, and by maintaining a more active and active mammary gland. secretory at the end of the experiment. These effects were probably caused by the increase in prolactin levels promoted by phytoestrogens in the ethanolic extract. Thus, Valerian is a promising source for use as a galactagogue phytotherapic by lactating women, however, further studies evaluating the mechanisms of action and toxicity should be carried out to justify its safe use by lactating women.

MEMBROS DA BANCA:
Presidente - 1553274 - REGIANE YATSUDA
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo à Instituição - ÉRIKA PEREIRA DE SOUZA - FTC