Banca de DEFESA: ADRIANO FERNANDES TEIXEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ADRIANO FERNANDES TEIXEIRA
DATA : 21/08/2020
HORA: 08:00
LOCAL: Instituto Multidisciplinar em Saude - Plataforma remota
TÍTULO:

Evaluation of the presence of dengue, Zika and Chikungunya in Aedes larvae collected in natura and documentation of simultaneous vertical transmission

PALAVRAS-CHAVES:

Coinfection; cotransmission; transovarial transmission; Aedes aegypti; Zika virus; dengue virus; Chikungunya virus.

PÁGINAS: 55
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Dengue, zika and chikungunya are arboviruses of great importance for public health worldwide. Aedes Aegypti is the principal vector for arboviruses in Brazil. The three arboviruses circulate simultaneously in the same geographic regions in a synergistic with for Aedes Aegypti, leading to considerable costs to health services and significant morbidity in the population. Aedes Aegypti is essential for the spread of arboviruses and the perpetuation of the pathogen in nature, it is necessary to understand the aspects of its reproduction. The aim of the study was to identify the circulation of Zika virus (ZIKV), Chinkungunya virus (CHIKV) and Dengue virus (DENV) in larvae hatced from eggs collected from traps (ovitramps) in Vitória da Conquista. The city was divided into 5 administrative regions. The Municipal Health Department guides the distribution of 50 ovitramps, according to the score, with the Rapid Study of Infestation Indexes by Aedes Aegypti (LIRAa) of each region. Egg collection took place between the months of April and May 2019. The eggs were matured and the larvae were fed fish food up to levels L2 to L3. Viral RNA was extracted from larvae and stored at -70 ºC until cDNA synthesis. An amplification of the genetic material occurred using the TaqMan 2x Universal PCR Master Mix system (Thermo Fisher Scientific, USA). There were 74% of ovitraps to be positive for the presence of eggs and 60% of these viable larvae. In total, 450 larvae were distributed in 30 pools of 25 larvae. In addition, 30 larvae were assessed individually. Fifty-three percent of the pools were positive for one of the arboviruses, 26% of the pools were positive for ZIKV, 13% for CHIKV and 10% for DENV. When the larvae were evaluated individually, the presence of a virus was detected in 30% of the samples, 13% positive for DENV and 3.3% ZIKV. Surprisingly, a co-transmission of DENV and ZIKV was detected in four larvae (13.3% of the samples) with the highest number of copies for ZIKV. There was a correlation with statistical significance between the LIRAa score and the presence of arboviruses in different regions. These data prove the co-circulation, presence of individual vertical transmission of DENV, ZIKV and CHIKV and transovarian co-transmission of DENV and ZIKV in nature

MEMBROS DA BANCA:
Presidente - 2315391 - FABRICIO FREIRE DE MELO
Interno - 2346070 - TIANA BAQUEIRO FIGUEIREDO
Externo ao Programa - 328.539.717-91 - PAULO FILEMON PAOLUCCI PIMENTA - USP
Externo à Instituição - BARBARA APARECIDA CHAVES