Banca de DEFESA: MAIARA PRATES DE ALMEIDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MAIARA PRATES DE ALMEIDA
DATA : 06/12/2019
HORA: 14:00
LOCAL: Instituto Multidisciplinar em Saúde
TÍTULO:

Obtaining tablets containing standard spray-dried hydroalcoholic extract from Lippia alba (Miller) N. E. Brown

PALAVRAS-CHAVES:

hydroalcoholic extract, Lippia alba, tablets

PÁGINAS: 95
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

One of the promising native species to develop a new drug is Lippia alba (Miller) N. E. Brown. This plant is popularly known as “erva-cidreira” and several pharmacological studies were carried out evaluating the activity of both its essential oils and the leaf extracts of its different chemotypes. Among the pharmacological activities of the plant are antiulcerogenic, antispasmodic, anesthetic and sedative, as well as antioxidants and antibacterial. Lippia alba presents as fixed constituents from iridoids, flavonoids and also phenylpropanoids. Acteoside is a glycosylated compound, a phenylpropanoid present in Lippia alba in high quantities than other substances and maybe an analytical marker for the plant. Its pharmacological actions range from antioxidant, anxiolytic and even neuroprotective activity. The present study aims for the development and chemical-physical characterization of tablets obtained from spray-dried Lippia alba hydroalcoholic extract. Molecular modeling analyzes of the structures of 15 compounds identified and quantified for the plant were submitted to the evaluation of anxiolytic and sedative activities with de aid of the PASS program. It was performed spray drying of the hydroalcoholic extract (SDLA) of the plant, the calculation of the Carr index (IC) and Hausner ratio (RH) of the obtained powder. The developing formulations with standardized extracts and the angle of repose analyses for the chosen were investigated. Compression was performed directly and subsequently, the parameters of mean weight, friability, hardness and disintegration of the tablets were analyzed. An analytical method was validated by HPLC to quantify acteoside. The solubility test os the active pharmaceutical ingredient, the extract, was performed from the quantification of the exposed acteoside in aqueous médium with pH variation from 1.0 to 6.8 after 24h. The extract and tablets were dosed and the final product was dissolved. A possible anxiolytic action of acteoside due to inhibition of the enzyme GABA transaminase is suggested by analysis of the results obtained with the PASS server prediction. SDLA 7 extract showed excellent results for IC and RH, being chosen for development. The tablets were approved for the tests of average weight, hardness, friability, and disintegration. The validity method used methanol and formic acid 0,9% (1:9) with the working range from 2.10 to 18.90 µg/mL, analyzed at 325 nm. The linearity, precision, selectivity, robustness, accuracy, and limits of quantification and detection were following current guidelines. The solubility test showed better results in HCl 0.1M. The dosage of the extract and tablets was approved, as well as the dissolution of the developed product.

MEMBROS DA BANCA:
Externo à Instituição - ANDRE LUIS MORAIS RUELA - UFOP
Interno - 1861045 - JULIANO GERALDO AMARAL
Externo à Instituição - MATEUS FREIRE LEITE - UNIFAL-MG