Banca de DEFESA: BRUNO PEREIRA DE ARAÚJO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : BRUNO PEREIRA DE ARAÚJO
DATA : 26/11/2019
HORA: 14:00
LOCAL: Sala de aula 401 da Pós Graduação
TÍTULO:

Study of the larvicide activity of curcumine by Photodynamic Therapy in different concentrations and blue LED (460nm) about wild Aedes mosquitoes

PALAVRAS-CHAVES:

Photodynamic Therapy, Curcumin, Aedes.

PÁGINAS: 78
GRANDE ÁREA: Outra
ÁREA: Multidisciplinar
RESUMO:

Introduction: In the first quarter of 2019, there were 273193 confirmed cases of dengue in the country, which represented an increase of 74% over the same period in last year. Also in 2019, in the same period, 15352 probable cases of chikungunya were register, with 2 deaths confirmed (1 in Bahia and 1 in Rio de Janeiro) and 14 deaths are under investigation. As for Zika, in this period, 2344 probable cases were register in the country, with the North and Southeast being the regions with the highest number of probable cases. It is notorious that current policies for vector control of these arboviruses are not effective. Studies of the use of light-activated photosensitizers as an alternative to conventional insecticides for sustainable control of vector mosquitoes such as Aedes (dengue, yellow fever, chikungunya), Anopheles (malaria), Culex (yellow fever) can already be found, showing advantages over conventional insecticides (efficacy, safety, non-mutagenicity and fast degradation). Objective: Evaluate the effectiveness of curcumin-mediated photodynamic therapy (PDT) and blue LED (460nm) on mortality of wild larvae of Aedes aegypti’s mosquitoes. Material and methods: Traps were place in strategic regions identified by the LIRA issued by the Secretariat of Epidemiological Surveillance of the city of Vitória da Conquista-BA. The larvae collected in stages L2 and L3 were feed for 24h with curcumin at concentrations of 10, 20 and 50% mixed with fish food and then underwent blue LED irradiation for 2h (22mW / cm2 and 158.4 J / cm2). The larvae were place in a container with chlorinated water and mortality was followed for 24 and 48h. Control groups in which only larvae were expose to blue LED for 2h and where larvae were only fed with curcumin mixed feed (10, 20 and 50%) were included in the study, in addition to the untreated group. All experiments were repeated after a 2-month interval for confirmation of the results, totaling 256 tests (tests 1 and 2, n = 16) between the PDT 10%, PDT 20%, PDT 50%, curcumin 10%, curcumin 20%, curcumin 50%, blue LED and untreated group.  Larvae belonging to the 20% PDT group were submitted to histological slides, confocal microscopy and Raman spectroscopy. Results: All PDT groups showed larvicidal activity, evidenced by the complementary tests performed in the PDT 20% group. Conclusion: The PDT in all concentrations was effective in larval mortality, with the concentration of 20% curcumin showing the best activity with mortality of 100% in 24h.

MEMBROS DA BANCA:
Presidente - 1564303 - FRANCINE CRISTINA SILVA ROSA
Interno - 1554172 - LUCIANO PEREIRA ROSA
Interno - 2315391 - FABRICIO FREIRE DE MELO
Externo ao Programa - 2575492 - AGDA ALVES DA ROCHA