Banca de DEFESA: SUZETE CARVALHO LANDULFO LUZ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : SUZETE CARVALHO LANDULFO LUZ
DATA : 03/06/2019
HORA: 09:00
LOCAL: IMS/CAT
TÍTULO:

PHOTODYNAMIC THERAPY: EFFECTIVENESS AND MOLECULAR MECHANISMS OF CURCUMIN ACTION AS FOTOSENSITIZER AGAINST Pseudomonas aeruginosa BIOFILM.

PALAVRAS-CHAVES:

Photodynamic Therapy. Curcumin. LED. Biofilm. Disruption. Pseudomonas aeruginosa

PÁGINAS: 123
GRANDE ÁREA: Ciências da Saúde
ÁREA: Medicina
SUBÁREA: Clínica Médica
ESPECIALIDADE: Doenças Infecciosas e Parasitárias
RESUMO:

Pseudomonas aeruginosa is a gram-negative bacteria with a high capacity to form dense biofilms, a complex structure that predisposes the development of antibiotic-resistant phenotypes, contributing to health-care-related infections becoming a serious public health problem worldwide. It is an in vitro research of the experimental type whose objective was to evaluate the effectiveness of different protocols of Photodynamic Therapy (PDT) mediated by curcumin against Pseudomonas aeruginosa biofilms that were submitted to protocols of PDT mediated by curcumin in three different concentrations (5μg / ml, 20μg / ml and 40μg / ml), activated by blue LED light at three different energy doses (3.24J / cm², 5.4J / cm² and 10.8J / cm²) compared to a negative control group ( without treatment) and positive control groups (LED and curcumin, alone, in the concentrations and energy doses delineated for the study), totaling 16 experimental groups. After checking the assumptions of normality and homogeneity, the statistical analysis was performed by Anova and Kruskal-Wallis, with post-test of Bonferroni and Dunn, respectively and the interpretation of the data took into account the values of reduction of Log10 of formative units colony count per ml (CFU / ml) to evaluate the antimicrobial effect of the therapy instituted. The biofilm structure analyzes were performed using confocal laser scanning (CSLM) images and Raman microspectroscopy allowed to evaluate the molecular mechanisms of curcumin action through the observed peaks in the spectrum. The results demonstrated an action centered on the disruptive effect of the biofilm, besides an antimicrobial effect in all PDT protocols, with better results observed in the protocols with higher concentration of photosensitizer and higher energy dose. The curcumin-mediated PDT evaluated in this research proved to be effective not only in reducing the viability of bacterial cells, but mainly because of its antibiofilm effect, and could be used as an antimicrobial potentiator.

MEMBROS DA BANCA:
Presidente - 1554172 - LUCIANO PEREIRA ROSA
Interno - 1564303 - FRANCINE CRISTINA SILVA ROSA
Externo à Instituição - NATALIA MAYUMI INADA