Banca de DEFESA: NIVEA NARA NOVAIS ANDRADE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : NIVEA NARA NOVAIS ANDRADE
DATA : 31/05/2019
HORA: 14:00
LOCAL: IMS- UFBA
TÍTULO:

 

IDENTIFICATION, CHARACTERIZATION OF RESISTANCE AND PATHOGENICITY OF Escherichia coli and Klebsiella spp. IN SAMPLES OF DONORS OF THE HUMAN MILK BANK OF THE MUNICIPAL ESAÚ MATOS HOSPITAL IN VITÓRIA DA CONQUISTA (BA).

PALAVRAS-CHAVES:

Pathogenicity factors, Virulence factors, Human milk, Enterobacteria, Microbiology, Neonatal infections.

PÁGINAS: 78
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
ESPECIALIDADE: Microbiologia Médica
RESUMO:

Breast milk (BM) is considered the ideal food for newborns (NBs). Due to its nutritional composition and immunibiological and protective factors, the World Health Organization (WHO) recommends exclusive breastfeeding up to six months.In some situations, such as NB stay in the hospital setting or maternal infectious diseases, it is not possible for NB to obtain BM due to breastfeeding and Human Milk Banks (HMB) emerge as a strategy to guarantee the nutrition of these NBs through the distribuition of milked human milk (MHM) provided by donors registered in the service. The MHM collection can be carried out at the donors' home and the hygienic-sanitary conditions used from milking to processing must follow protocols to avoid microbiological contamination since BM possesses ideal characteristics for the growth of several microorganisms that can be pathogenic to predominantly premature receptors. Among the bacteria that can contaminate the MHM in the milking, transportation and processing stages are Enterobacteria, which are mostly commensal, but have the capacity to acquire pathogenicity and antimicrobial resistance genes. In this family the species of Klebsiella spp and Escherichia coli are important cause of neonatal infections such as meningitis, pneumonia, intestinal infections and sepsis. Therefore, the present study aimed to identify and characterize the pathogenicity and antibiotic resistance profile of E. coli and Klebsiella spp. in samples of donors from the HMB of the Esau Matos Hospital and to correlate the microbial presence with the hygienic-sanitary conditions of the study participants. Samples were collected from the hands and nipple-areolar region, raw human milk aliquot and human milk aliquot after the pasteurization of 30 donors registered at the HMB during the year 2018. The donors also filled out a questionnaire about their socioeconomic and hygienic-sanitary conditions of milking. Samples were seeded using a conventional microbiology technique and strains of Klebsiella were identified in samples from 11 donors (36.6%). Of these, in 36.6% (n = 4) the microorganism was isolated only in cutaneous samples, 36,6% (n = 4) only in raw milked human milk raw (MHMR), 18.2% (n = 2) with positive results in cutaneous and MHMR samples and 9.1% (n = 1) with growth in the three seed samples. E. coli was not isolated in the samples collected. In all strains of Klebsiella spp. the antibiogram with recommendation of the CLSI 2017 was performed. 42.8% (n = 6) of the strains showed resistance to Ampicillin and Sulbactam and 35.7% (n = 5) resistant to Amoxicillin and Clavulanic Acid. The isolates were also subjected to the phenotypic detection of ESBL by disk-approximation, 23.1% (n = 3) presented positive results for this test. The modified Hodge test was performed on all strains to evaluate the presence of the carbapemenase enzyme and all strains tested negative. In addition to the phenotypic tests, the isolates were submitted to the Polymerase Chain Reaction technique to detect the following genes: gyrA, rpoB and pehX, for confirmation of the genus Klebsiella spp. and species of Klebsiella pneumoniae and Klebsiella oxytoca respectively; the genes associated with the production of extended spectrum beta-lactamases (ESBL) SHV, TEM, CTXM-1, CTXM-2 and CTXM-3 and the virulence genes rmpA and wcaG were also investigated. All strains were confirmed as Klebsiella spp., 53.8% (n = 7) identified as Klebsiella pneumoniae, 7.7% (n = 1) identified as Klebsiella oxytoca and 38.5% (n = 5) are not part of the two species surveyed. No ESBL genes and virulence genes were found in the isolated samples.

MEMBROS DA BANCA:
Presidente - 1739574 - MARCIO VASCONCELOS OLIVEIRA
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo ao Programa - 3224857 - CLAUDIO LIMA SOUZA
Externo ao Programa - 1034133 - JESSICA BOMFIM DE ALMEIDA