Banca de DEFESA: MARISOL PORTO ROCHA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARISOL PORTO ROCHA
DATA : 17/06/2019
HORA: 14:00
LOCAL: Sala da Pós Graduação IMS/UFBA
TÍTULO:

DEVELOPMENT AND EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF ORAL MICROEMULSIONED RINSE CONTAINING CURCUMIN FOR INTRAORAL PHOTODYNAMIC THERAPY

PALAVRAS-CHAVES:

Photodynamic Therapy; Curcumin; Microemulsion; Nanostructured system

PÁGINAS: 90
GRANDE ÁREA: Outra
ÁREA: Tecnologia e Inovação
RESUMO:

PDT (Photodynamic Therapy - PDT) uses a light source, a photosensitizer (FS) and oxygen in the reaction medium to produce as a result highly reactive species capable of causing unfeasibility of microorganisms, such as: bacteria and fungi. The objective of this work was to develop a microemulsion based rinse formulation for dental use in PDT. FScurcumine was incorporated into the formulation and its antimicrobial potential was evaluated using PDT. After the construction of a pseudoternario phase diagram, oil-in-water (O / W) microemulsion composed of polysorbate 80, linseed oil, sobitol, polyethylene glycol 400 and water as an appropriate vehicle for curcumin incorporation. The selected systems were characterized by measurements of conductivity, rheological profile, droplet size and zeta potential. The physical stability of the formulation was also evaluated. The antimicrobial evaluation of the developed mouthwash was evaluated against PDT. A total of 360 acrylic resin specimens were divided into groups (n = 10) with curcumin concentrations of 80 and 40 μg / mL and irradiation times of 10 and 30 minutes. The specimens were contaminated with Staphylococcus aureus methicillin resistant strains, Candida albicans and Escherichia coli in wells of culture medium containing BHI or Sabouraud medium and incubated at 35 ± 2 ° C for 24 hours for the formation of biofilms. After incubation, the experimental groups were treated with PDT, using 460 nm LEDs for activation of FS present in the oral mouthwash. Three other groups were added: control in which no treatment was performed, only the FS and only the FS. The results showed effectiveness above 5Log10 in the groups treated with PDT 40 and 80μg / mL in the time of 30 minutes.

MEMBROS DA BANCA:
Presidente - 1564303 - FRANCINE CRISTINA SILVA ROSA
Interno - 1554172 - LUCIANO PEREIRA ROSA
Externo à Instituição - ANDRE LUIS MORAIS RUELA - UFOP
Externo à Instituição - NATALIA MAYUMI INADA - USP