Banca de DEFESA: CAROLINE SCABINI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAROLINE SCABINI
DATE: 15/04/2024
TIME: 14:00
LOCAL: Instituto Multidisciplinar em Saúde – IMS CAT-UFBA
TITLE:

Seasonal and circadian variation of non-volatile metabolites in Lippia alba leaves (Mill.) N.E.Br. ex Britton & P. Wilson

KEY WORDS:

acteoside; Iridoids; liquid chromatography; natural products; rainfall; temperature; tricin-7-O-diglucuronide

PAGES: 55
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

This study describes the seasonal and circadian variations and the major compounds from Lippia alba leaves by HPLC analysis. This plant is widely used as a phytomedicine by the locals, and the appropriate harvesting time is a key factor for achieving phytochemical quality and meeting consumer needs. The Statistical Package for the Social Science software (SPSS) and ANOVA were used to identify, quantify, and associate the variations in the secondary metabolites of this species through chromatographic analysis. Hydroethanolic extracts from the leaves of six specimens from L. alba were prepared. For the circadian study, the samples were collected at four different hours of the day in each year's season (2018). For the seasonal study, the samples were collected monthly from the same individuals for two consecutive years (2018 and 2019).These samples were analyzed and quantified using a validated HPLC method for flavonoids, iridoids, and phenyl ethanoid glycoside. The data analysis revealed that in mild temperatures between 6:00 pm and 6:00 am an enhancement in the production of secondary metabolites was observed, and spring and summer had the highest yields of them (2018 and 2019). Mussaenoside, acteoside, and tricin-7-O-diglucuronide showed a moderate positive correlation between their production and the precipitation index, while epi-loganin had a moderate negative correlation. Acteoside showed a moderate positive correlation between the minimum temperature and its production. Compared with previous studies, a drastic reduction (about 95%) in the production of tricin-7-O- diglucuronide was observed from 2015 to 2019, which could be attributed to the plant's aging. We demonstrated that lower temperatures and high rainfall could favor the production of the major L. alba active compounds (acteoside and tricin-7-O- diglucuronide) and that older plants harm their production. These results provide unprecedented information about the composition of this species, which is relevant for its large-scale cultivation to supply plant material with higher levels of bioactive for the production of phytomedicines.

COMMITTEE MEMBERS:
Presidente - 2585200 - ANGELICA FERRAZ GOMES
Interno - 1788171 - BRUNO OLIVEIRA MOREIRA
Interno - 1861045 - JULIANO GERALDO AMARAL
Externa ao Programa - 1552462 - MARILUZE PEIXOTO CRUZ - null