Banca de DEFESA: THAYSE PITHON QUADROS RAVAZZI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : THAYSE PITHON QUADROS RAVAZZI
DATE: 13/12/2022
TIME: 09:00
LOCAL: IMS-UFBA
TITLE:

Antimicrobial Photodynamic Therapy (aPDT) with nanotechnological curcumin and 450nm blue LED on multispecies biofilms in root canals

KEY WORDS:

Photodynamic therapy; Endodontics; LED; Curcumin; Nanotechnology; Biofilm

PAGES: 58
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Endodontia
SUMMARY:

The eradication of microorganisms in the root canals is one of the main goals of endodontic therapy and is related to the success of the treatment. Antimicrobial Photodynamic Therapy (aPDT) has emerged as a new therapy that aims to eliminate persistent microorganisms in the chemical-mechanical preparation. The aim of this study was to analyze the effectiveness of using aPDT with nanotechnological curcumin activated by blue LED (450nm) on the elimination of microorganisms organized in multispecies biofilm, inside the root canals of natural human teeth in vitro. Sectioned roots of 40 single-rooted human teeth were used, which were randomized into experimental groups (n=10): control group – no treatment; photosensitizer group (FS) – nanotechnological curcumin used alone; Light group – blue LED used alone; and aPDT group – nanotechnological curcumin activated by blue LED. For the tests, the inside of the conduits was contaminated by a multispecies biofilm transposition technique consisting of ATCC species of Candida albicans (ATCC 90029), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), and methicillin-resistant Staphylococcus aureus- MRSA (ATCC 43300). After the incubation period, the microrganisms were treated according to the experimental groups, and in the control group there was no treatment, but the collection was carried out by aspirating 1mL of the root canal content that was seeded in the selective media sabouraud dextrose agar with chloramphenicol, mitis salivarius bacitracin sucrose agar, Mac Conkey agar, mannitol agar and incubated in a B.O.D. for 24 hours at 36°C. Subsequently, the CFU/mL were counted. In the FS group, 80µg of curcumin was added to the root canal, the pre-irradiation time was waited and the same procedures for collection, seeding of the root canal content and CFU/mL count were performed; in the light group, the entrance of the channels was irradiated with blue LED for 5 min (450nm; 1000 mW/cm2 ; 300J/cm2 ) and after this step, collection, seeding of the content of the channels and counting of CFU/mL were performed. In the aPDT group, the conduits were filled with FS, the pre-irradiation time was waited, and the irradiation was performed with blue LED for 5min, and later the conduit contents were collected, seeded and the CFU/mL were counted. The data on the reduction in the number of Log10 CFU/mL of the microorganisms studied were compared between the groups tested using the ANOVA statistical test (5%) with Tukey's posttest. It was concluded that the treatment with aPDT using nanotechnological curcumin was effective in reducing all microorganisms studied in the form of multispecies biofilm, and the average reduction was greater than 1.5 Log10 for all microorganisms, except Candida albicans, although the latter also showed a reduction.

COMMITTEE MEMBERS:
Presidente - 1564303 - FRANCINE CRISTINA SILVA ROSA
Interno - 1554172 - LUCIANO PEREIRA ROSA
Externo à Instituição - ROGERIO VIEIRA SILVA