Banca de DEFESA: VINICIUS QUEIROZ OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : VINICIUS QUEIROZ OLIVEIRA
DATE: 08/12/2022
TIME: 14:00
LOCAL: IMS/CAT/UFBA
TITLE:

Antitumor and antiangiogenic effect of the BthMP, a metalloproteinase from Bothrops moojeni venom

KEY WORDS:

Snake venom, cancer, angiogenesis, metalloproteinase, Bothrops moojeni

PAGES: 85
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Química de Macromoléculas
SPECIALTY: Proteínas
SUMMARY:

Lung cancer is the second most prevalent type of cancer and the first in number of deaths worldwide. In this context, several studies have shown that toxins isolated from snake venom have been investigated with the aim of developing future drugs for cancer therapy. Thus, we evaluated the antitumor and antiangiogenic effects of BthMP metalloprotease isolated from Bothrops moojeni venom on human umbilical cord endothelial cell line (HUVEC) lung cancer cells. BthMP was cytotoxic to lung cancer cells (A549) showing inhibition of colony formation by the clonogenic assay and increased levels of LDH at concentrations of 40 and 5μg/mL. Furthermore, at these same concentrations, BthMP inhibited the invasion, migration and adhesion of A549 cells in addition to increasing the levels of reactive oxygen and nitric oxide species. Interestingly, the toxin did not interfere in the cytotoxicity and adhesion processes of non-tumorigenic lung cells. BthMP inhibited the adhesion and migration of HUVECs and blocked angiogenesis in vitro dependent on VEGF, an essential pro-angiogenic factor. In addition, the toxin was able to inhibit the angiogenic process through an ex vivo assay of the aortic ring. Finally, it was possible to observe in the A549 cell migration assay that the inactivation of the catalytic activity of the toxin abolished the inhibitory effect of the toxin, demonstrating that the antitumor activity of the metalloprotease is associated with its catalytic activity. Therefore, these results demonstrate that BthMP toxin has a significant antitumor and antiangiogenic effect on lung cancer and endothelial cells and represents an important biotechnological tool targeting a new form of cancer therapy..

COMMITTEE MEMBERS:
Presidente - 1071194 - DAIANA SILVA LOPES
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo ao Programa - ***.485.118-** - PATRÍCIA BIANCA CLISSA - USP