Banca de DEFESA: NATHÁLIA FREITAS FREIRE

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : NATHÁLIA FREITAS FREIRE
DATE: 21/12/2022
TIME: 08:30
LOCAL: videoconferência na plataforma RNP (sala PEI-UFBA)
TITLE:

DEVELOPMENT OF BIOBASED POLYMERIC NANOPARTICLES CONTAINING ACTIVE PHARMACEUTICAL INGREDIENTS FOR ANTICANCER APPLICATION.

KEY WORDS:

Biobased monomer; Poly(thioether-ester) nanoparticles; Thiol-ene in situ miniemulsion polymerization; Solvent evaporation polymerization; Zinc phthalocyanine; Full spectrum Cannabis extract; Cancer therapy; in vitro studies.

PAGES: 120
BIG AREA: Engenharias
AREA: Engenharia de Produção
SUMMARY:

Vegetable oils are potential substitutes for petroleum derivatives, as they present themselves as a clean and environmentally friendly alternative and are available in high quantities at relatively low prices. Biomass-derived chemicals can be converted into monomers with unique structures, generating materials with new properties. Methyl 10-undecenoic acid, a castor oil derived, was shown to be a suitable starting material for the preparation of esters with alkene groups that can be lead in biodegradable polymers. The main objective of this research work is the study of the synthesis and physical, chemical, and biological characterization of polymeric particles derived from renewable sources, containing active pharmaceutical ingredients (APIs) for biomedical application. At the first step of the work, the encapsulation of zinc phthalocyanine (ZnPc) in poly(thioether-ester) (PTEe) nanoparticles via thiol-ene miniemulsion polymerization using a biobased α-ω-diene diester monomer (made by esterification of 10-undecenoic acid and 1,3- propanediol, derived from castor oil) was obtained. Two different thiols were employed as comonomers. ZnPc loaded PTEe nanoparticles presented an average diameter of around 122 - 145 nm and high encapsulation efficiency (> 95%) of ZnPc for both thiols. FTIR and thermal analyses showed that there was no significant interaction between the drug and the polymer, indicating that ZnPc is molecularly dispersed in the polymeric matrix. The ZnPc release profile in the nanoparticles showed an initial burst effect followed by a slow-release rate. Cytotoxicity studies showed that nanoparticles do not cause hemolytic damage and showed phototoxic effect in breast cancer cells at low concentrations of ZnPc. In second step, full-spectrum Cannabis extract was encapsulated in PTEe nanoparticles synthesized by thiol-ene in situ miniemulsion and solvent evaporation technic with the biobased monomer (1,3-propylene diundec-10- enoate). The Full-spectrum Cannabis extract was characterized qualitatively and quantitatively by Gas Chromatography. The nanoparticles produced were characterized by particle size, surface charge, FTIR, DSC, TGA, electronic microscopy and encapsulation efficiency. Also, several in vitro studies as cytotoxicity assay, hemolysis assay, apoptosis, and membrane potential were accomplished to analyze the potential of nanoparticles for anticancer therapy treatments. With high encapsulation efficiency (> 97%) and an average diameter between 91 and 229 nm, the encapsulation of full-Cannabis extract in biobased nanoparticles was efficient to stimulate a cytotoxic effect on cancer cells line.

COMMITTEE MEMBERS:
Presidente - 1677204 - ELAINE CHRISTINE DE MAGALHAES CABRAL ALBUQUERQUE
Interna - 1568172 - ROSANA LOPES LIMA FIALHO
Externo à Instituição - Bruno Oechsler - UFSC
Externo à Instituição - Guillermo Velasco Diaz - UCMADRID
Externa à Instituição - ISLANE DO ESPIRITO SANTO
Externa à Instituição - RAQUEL DE MELO BARBOSA