The demand for safe natural products has increased over the years, which accelerated the food industry's enthusiasm for for the production of natural dyes in substitution to synthetics, making it possible to meet the demands of consumers. Therefore, the use of microorganisms as a source for obtaining pigments becomes a good alternative, requiring the development of studies that analyze the best conditions for production, with lower costs and greater yield. The light is a crucial environmental factor for regulating developmental and physiological processes in most organisms. The objective of this work was to evaluate the effect of luminosity (darkness and different color light) quality on biomass and extracellular pigment yield of Pseudofusicoccum sp. in submerged fermentation and to verify the in vitro cytotoxicity of the submerged extract in tumor and normal cell lines. The growth of Pseudofusicoccum sp. was statistically not affected by the incubation under different wavelengths of light. Pigment production has increased according to the color of light, white<blue<green<red<darkness<yellow. Extracellular pigment production resulted maximum in yellow light and total darkness (maximum absorbance value of 3.56 and 3.03 respectively) and the opposite was observed in unscreened white light (maximum absorbance value of 0.06). The pigment extract is not cytotoxic against cells lines tested (HepG2, SCC4, BJ and MRC-5), showing potential for use in food industry.