Banca de DEFESA: BIANE OLIVEIRA PHILADELPHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : BIANE OLIVEIRA PHILADELPHO
DATA : 12/02/2021
HORA: 14:00
LOCAL: Salvador
TÍTULO:

Antiproliferative activity of protein hydrolysates and fractions derived from soybean and cowpea beans proteins, on tumor cell lines, in vitro.

PALAVRAS-CHAVES:

Keywords: beta-conglycinin, beta-vignin, bioactive peptides; cytotoxicity, MDA-MB-231.

PÁGINAS: 93
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
ESPECIALIDADE: Química, Física, Fisico-Química e Bioquímica dos Alim. e das Mat-Primas Alimentares
RESUMO:

Several studies have shown that peptides derived from proteins in legume grains have beneficial effects on human health. Among these, the antioxidant, antilipidemic, anti-glycemic and anti-obesity action stands out. In this sense, more recent studies have suggested that peptide fractions appear to modulate the cell proliferation of some tumor strains. In this sense, the present study evaluated the cytotoxicity (antiproliferative activity) of protein hydrolysates and peptide fractions from proteins isolated from soy (Glycine max) and cowpea (Vigna unguiculata), on tumor lines, in vitro. Proteins glycine (11S) and β-conglycinin (7S) from soybeans, and β-vignin (7S) from cowpea were isolated, through solubilization and precipitation steps at the isoelectric point, and afterwards, were partially purified by process chromatographic. Then, the proteins glycinin and β-conglycinin were hydrolyzed by the sequential action of the enzymes pepsin / pancreatin, and β-vignin by different enzymatic systems: i. pepsin, ii. trypsin, iii. pepsin / pancreatin, and iv. alkalase / pepsin. Subsequently, the cytotoxicity of the hydrolysates was evaluated. None of the hydrolysates had a cytotoxic effect on human non-tumor cells (HUVEC) at concentrations of 12.5–200 µg / mL. On the other hand, β-conglycinin and glycine protein hydrolysates inhibited the cell proliferation of human breast adenocarcinoma (MDA-MB-231), human hepatocellular carcinoma (Hep-G2) and prostate carcinoma (DU-145), between 24% 54%, and 20% to 45%, respectively. In addition, β-vignin protein hydrolysates, derived from the action of pepsin (IC50 = 3.71 µg / mL) and trypsin (IC50 = 3.02 µg / mL) exerted an anti-proliferative action of -95% and -91% , respectively, on the MDA-MB-231 strain. For soybeans, the fraction of peptides between 10-3 kDa of β-conglycinin showed a more significant antiproliferative effect on MDA-MB-231 (IC50 7.4 µg / mL) and DU-145 (IC50 6.0 µg / mL), while the fraction <3 kDa had a better effect against Hep-G2 cells (IC50 5.7 µg / mL), both with dose-dependent effect. In cowpea, the peptide fraction between 10-3 kDa had the best effect against MDA-MB-231 cells (IC50 = 0.62 µg / mL), while the peptide fraction of 30-10 kDa had the best effect inhibitory in Hep-G2 cells (IC50 = 10.63 µg / mL). The results observed in this study indicate the presence of peptides in the fraction between 10-3 kDa, derived from the protein β-conglycinin of soy and β-vignin of cowpea, with antiproliferative action on tumor cell lines, especially for breast adenocarcinoma. However, further studies are needed in order to identify the peptides that exert this effect, and to clarify the mechanism (s) involved in cell death. These issues are currently being studied by our research group.

MEMBROS DA BANCA:
Presidente - 2091662 - EDERLAN DE SOUZA FERREIRA
Externo(a) à Instituição - FERNANDO ROGÉRIO PAVAN - UNESP
Externo(a) à Instituição - GUSTAVO GUADAGNUCCI FONTANARI - UFRA