Banca de DEFESA: DANILO ALVES SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANILO ALVES SILVA
DATA : 11/02/2021
HORA: 14:00
LOCAL: Faculdade de Farmácia
TÍTULO:

Production and Characterization of Chlorella vulgaris biomass in Produced Water

PALAVRAS-CHAVES:

Biofuels, chemical composition, pigments, fatty acids, microalgae, bioremediation.

PÁGINAS: 85
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
ESPECIALIDADE: Química, Física, Fisico-Química e Bioquímica dos Alim. e das Mat-Primas Alimentares
RESUMO:

The growing industrialization has caused changes in the environment, especially water resources, which has shown high concentrations of chemical compounds, coming from urban, agricultural and industrial activities, through the production of waste water. Currently, there are several types of industrial effluents, based on the numerous types of environmental contaminants and industries. Among these effluents, there is the water produced (AP), generated from the extraction of oil, it comes from the drilled well, therefore it has a complex composition containing organic and inorganic chemical compounds. Its production increases as the production of oil declines. oil and gas, therefore, for the extraction of crude oil it is necessary to increase the amount of water introduced. Among the treatments used to treat PA, the use of microalgae makes it possible to reduce environmental impacts, due to the bioremediation profile, in addition to producing biomolecules of commercial interest. The Chlorella genus has great biotechnological potential due to its metabolic profile, which is capable of producing large amounts of proteins, carotenoids (astaxanthin, lutein, β-carotene, violaxanthin and zeaxanthin), vitamins, minerals, antioxidants, polysaccharides, peptides and fatty acids . It is considered a promising source for the production of biofuels, known as the third generation. In this context, the objective of this study was to produce and characterize the Chlorella vulgaris biomass cultivated with daily supplementation of produced water (AP), aiming beyond the well-known bioremediation character of the microalgal species, to verify the production of biomolecules of commercial interest. Cultures were carried out in Erlenmeyer-type photobioreactors containing 1.5 L of BG11 medium, submitted to AP (daily supplementation with 50 mL of non-autoclaved water) and AD (daily supplementation with 50 mL of distilled water) treatment for 26 d. Biomass production was 4.87; 1.69 and 1.95 gL-1 in the control treatment, AP and AD, respectively. The values of µ specific and productivity for the Control, AD and AP, were respectively (0.24; 0.34 and 0.25 d- 1) and (0.03; 0.04 and 0.01 gL-1d-1). The biomass of the AP treatment had a higher carbohydrate content (40.19%), compared to the control (17.00%) and AD (22.50%). The values of chlorophyll a and b for AP ranged from 0.65 to 0.42μg / mL-1 and carotenoids 2.4 μg / mL-1, showing a significant difference (p <0.05) when compared to the Control (1.20 ( chlorophyll a), 0.80 (chlorophyll b) and 3.40 μg / mL-1 carotenoids), and AD (0.30 chlorophyll a; 0.20 chlorophyll b and 0.90 μg / mL-1 carotenoids). of AD lipids (24.08%) showed a significant difference (p <0.05) compared to AP (21.53%) and control (21.37%). The production of bioethanol in AP (18.22mL / 100g of biomass) was higher when compared to AD (10.20 mL / 100g of biomass) and to the control (7.70 mL / 100g of biomass). The fatty acid profile of AP was mostly composed of monounsaturated (48.52%), while AD (49.71%) and control (50.23%) saturated. AP showed a higher content of palmitoleic acid (5.42 mg^.g-1); γ-linolenic (3.02mg^.g-1) and linolelaidic (1.94 mg^.g-1). As for the biodiesel properties, AP presented cetane number (49.22%) and iodine value (101.32g iodine / 100g) according to the norms. The levels of saponification 211.97 compared to 1st generation biofuels, only the plugging point, was slightly higher (22.48 ° C) than recommended by the legislation. In addition, a high removal efficiency of heavy metals such as Copper (Cu), Manganese (Mn), Molybdenum (Mo), Zinc (Zn) and Iron (Fe) which obtained removal efficiency of 70.00%, 76.89 %, 97.82%, 96.55% and 90.74% respectively, and 48.59% total hydrocarbon removal (HTP).Therefore, the microalgae Chlorella vulgaris is a promising alternative for bioremediation of chemical compounds in the water produced, generating biomass that can be used as a substrate for the production of biofuels, as shown in the AP treatment using wastewater from the oil industry.

MEMBROS DA BANCA:
Presidente - 1350842 - JANICE IZABEL DRUZIAN
Interno(a) - 1980101 - MARCELO ANDRES UMSZA GUEZ
Externo(a) à Instituição - JÉSSICA HARTWIG DUARTE - UFRN