Banca de QUALIFICAÇÃO: BIANE OLIVEIRA PHILADELPHO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : BIANE OLIVEIRA PHILADELPHO
DATA : 18/01/2021
HORA: 08:00
LOCAL: Salvador
TÍTULO:

β-conglicinin peptide fractions exerts inhibitor effect on the proliferation of MDA-MB-231, Hep-G2 and DU-145 cancer cells, in vitro

PALAVRAS-CHAVES:

Soybean proteins. β-conglicinin hydrolysate. Antiproliferative activity. tumor cell lines.

PÁGINAS: 22
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
RESUMO:

Peptides derived from soy proteins have received remarkable interest due to its potential antitumor activity. Therefore, the purpose of this study was to assess the possible antiproliferative effect of glycinin and β-conglycinin hydrolysates and peptide fractions. β-conglycinin and glycinin are major storage proteins from soybean were isolated, partially purified by chromatographic process and hydrolysed by sequential action (pepsin/pancreatin). Both glycinin and β-conglycinin hydrolysates were not cytotoxic to non-cancer human cell HUVEC (concentrations 12.5-200 µg/mL), in vitro. β-conglycinin hydrolysate exhibited the highest antiproliferation activity (between 24 to 54%), compared glycinin (between 20 to 45%) against human mammary adenocarcinoma (MDA-MB-231), human hepatocellular carcinoma (Hep-G2), prostate carcinoma (DU-145) cell lines. The 10-3 kDa peptide fraction from β-conglycinin hydrolysate showed the strongest antiproliferative effect on MDA-MB-231 (between 15 to 63%, IC₅₀ 7.4 µg/mL) and DU-145 (between 33 to 60%, IC₅₀ 6.0 µg/mL), whereas the < 3 kDa fraction showed better effect against Hep-G2 (between 35 to 63%, IC₅₀ 5.7 µg/mL) cells, in vitro. In additional, the antiproliferative activity observed was in a dose-response manner. Future studies should focus especially to identify peptides responsible for its antiproliferative activity. Some of these issues are currently being explored in our laboratory.

MEMBROS DA BANCA:
Presidente - 2091662 - EDERLAN DE SOUZA FERREIRA
Externo(a) à Instituição - GUSTAVO GUADAGNUCCI FONTANARI - UFRA
Externo(a) à Instituição - JULIANA CRISTINA BASSAN - UNESP