Effect of luminosity on extracellular pigment production by Pseudofusicoccufm sp. and cytotoxity activity evaluation
colorant, endophytic, submerged fermentation
Consumers are increasingly interested in safe natural products, which has accelerated the food industry's enthusiasm for obtaining natural pigments. Therefore, the use of microorganisms to produce pigments has increased. The light is a crucial environmental factor for regulating developmental and physiological processes in most organisms. The objective of this work was to evaluate the effect of luminosity (darkness and different color light) quality on biomass and extracellular pigment yield of Pseudofusicoccum sp in submerged fermentation and to verify the in vitro cytotoxicity of the submerged extract in tumor and normal cell lines. The growth of Pseudofusicoccum sp. was statistically not affected by the incubation under different wavelengths of light. Pigment production has increased according to the color of light, white<blue<green<red<darkness<yellow. Under yellow light and total darkness resulted in highest pigments production the maximum absorbance value was observed at the 420 nm spectrum with yellow light (3.56) and the lowest result was observed at the 620 nm spectrum with white light (0.06), low pigment production was observed in the maximum absorbance spectrum of blue pigments (0.46 was the maximum absorbance value. The pigment extract is not cytotoxity against cells lines tested, showing potential for use in food industry.