Banca de DEFESA: ANDRESSA DE OLIVEIRA CERQUEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANDRESSA DE OLIVEIRA CERQUEIRA
DATA : 25/09/2019
HORA: 09:00
LOCAL: Sala 07 Faculdade de Farmácia
TÍTULO:

Determination of acrylamide in acarajé and potato chips by gas chromatography coupled with mass spectrometry (GC-MS) and its risk assessment for human consumption

PALAVRAS-CHAVES:

Acrylamide, acarajé, chips, CG-MS, health hazard.

PÁGINAS: 80
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
SUBÁREA: Bromatologia
RESUMO:

Different methodologies are proposed for the identification and quantification of acrylamide (AA) in food, a potentially neurotoxic and carcinogenic byproduct formed between amino acids and reducing sugars in the Maillard reaction. Since the first release in 2002 of the relationship of its formation in foods that undergo high temperature treatment, a range of products such as coffee, breakfast cereals, potato-based foods and cookies has been explored. However, there is no data in the literature regarding the occurrence of AA in acarajé, a traditional snack of the Bahian cuisine. Animal studies have shown that AA is rapidly absorbed by all exposure routes and is faster in GI tract. Given the degree of hazard and toxicity to the consumer, its detection and quantification is very important. This work proposed to validate analytical methodology for the quantification of AA in samples of acarajé and chips and subsequently perform a health hazard assessment based on the intake of these food items. Isotopically labeled d3-acrylamide was used as the internal standard. The method involved extraction with isooctane and acetonitrile, followed by concentration and derivatization with xanthydrol. Gas chromatographic analysis with mass spectrometry (GC-MS) was used in selective ion monitoring mode. According to the established conditions, the detection and quantification limits were 3 and 10 μg/L, respectively. The recoveries ranged from 98 to 104%. The proposed analytical method was applied to eighteen samples of acarajé sold in Salvador, Bahia, Brazil and nine samples of potato chips from three main fast food chains. AA contents ranged between 16.4 to 49.9 μg/kg in the acarajés from several points of sale. In potato chips, we observed a significant difference in AA levels according to the fast food branch, with a minimum value of 48.9 and a maximum of 414.0 μg/kg. Health concern has been estimated for an AA intake of more than 1 µg/kg bw per day, and translated into an EOM of 10,000 according to EFSA (2015). Thus, the consumption of a portion of high AA content potato chips as observed in this study may represent a carcinogenic risk (EOM = 1866). On the other hand, the excessive consumption of acarajé, three or more units per week may also be associated with this long-term risk. It is important to expand the investigation, analyzing a larger number of samples and other food items. In that way, the contribution of acrylamide to the total diet can be calculated and consequently the possible health risks to the Brazilian population related to the dietary exposure to this contaminant are more propely estimated.

 

MEMBROS DA BANCA:
Presidente - 2181841 - JOSE ANTONIO MENEZES FILHO
Interno - 1475020 - MARIA EUGENIA DE OLIVEIRA MAMEDE
Interno - 2091662 - EDERLAN DE SOUZA FERREIRA
Externo à Instituição - ADRIANA PAVESI ARISSETO BRAGOTTO - UNICAMP