Banca de DEFESA: ANISIO IURI LIMA DOS SANTOS ROSÁRIO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANISIO IURI LIMA DOS SANTOS ROSÁRIO
DATA : 29/04/2019
HORA: 14:00
LOCAL: Faculdade de Farmácia (sala 07)
TÍTULO:

Occurrence and antimicrobial resistance of  shiga toxin-producing Escherichia coli isolated from dairy products in Bahia, Brazil

PALAVRAS-CHAVES:

STEC; stx1 and stx2 genes; foodborne disease; food safety; public health.

PÁGINAS: 60
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
ESPECIALIDADE: Microbiologia de Alimentos
RESUMO:

Coliform bacteria are a group of microorganisms that indicates poor hygiene practices when found in food. Within this group, Escherichia coli in food may indicate fecal contamination, besides representing the presence of potential pathogens that may cause human diseases. While most species are commensal, known pathogenic groups such as Shiga toxin-producing E. coli (STEC) represents a public health concern due to its ability to produce Stx1 and Stx2 toxins, being able to cause intestinal and extraintestinal diseases. Therefore, monitoring milk production stages and checking the innocuity of dairy products are crucial for food safety. In this context, this study aimed to verify the prevalence of thermotolerant coliforms, E. coli and STEC strains, besides evaluating the resistance profile to 12 antimicrobials used in both veterinary and human medicine. A total of 123 dairy samples were obtained from 15 different locations in the state of Bahia, Brazil (Baixa Grande, Cachoeira, Camaçari, Castro Alves, Feira de Santana, Ichú, Ipirá, Irecê, Mundo Novo, Pedrão, Riachão do Jacuípe, São Sebastião do Passé, Simões Filho, Teodoro Sampaio, and Wanderley). The samples were submitted to coliform assay and streak plating on Eosin Methylene Blue agar. Positive E. coli strains were submitted to duplex and multiplex polymerase chain reaction for STEC confirmation and serotyping of non-O157, respectively. Confirmed STEC strains were also tested to 12 antibiotics by disk diffusion technique. Unsatisfactory levels of thermotolerant coliform were observed in 16% of the tested samples, and a total of 9 samples had the presence of E. coli. In addition, 9 STEC strains were isolated from pasteurized cream (2/9), minas padrão cheese (2/9), minas frescal cheese (4/9), and ricotta (1/9). Two STEC strains exhibited resistance to streptomycin, whereas five strains presented an intermediary resistance to ampicillin, including the two aforecited. The presence of STEC strains in milk and milk products represents a risk to the consumer, especially the young and elderly, and contamination can occur during different stages of milk chain.

MEMBROS DA BANCA:
Presidente - 1142664 - MARION PEREIRA DA COSTA
Interno - 2358665 - CARLOS PASQUALIN CAVALHEIRO
Externo à Instituição - NELSON DE CARVALHO DELFINO