Banca de DEFESA: LARISSA SANTOS ASSUNCAO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LARISSA SANTOS ASSUNCAO
DATE: 05/01/2024
TIME: 14:00
LOCAL: On-line
TITLE:

NANOENCAPSULATION OF UNAUÉ HIE OxG PALM OIL HYBRID WITH VEGETABLE COPRODUCTS: OPTIMIZATION, CHARACTERIZATION, CELLULAR ANTIOXIDANT ACTIVITY ASSESSMENT, AND CYTOTOXICITY.

KEY WORDS:

Elaeis guineensis. Elaeis oleifera. High-oleic palm oil. Nanoparticles. Antioxidant potential.

PAGES: 110
BIG AREA: Ciências Agrárias
AREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
SUMMARY:

The search for innovative alternatives in preserving bioactive compounds, such as those found in azeite de dendê híbrido or hybrid crude palm oil (HCPO) Unaué HIE OxG, stands out due to its rich composition of carotenoids, tocopherols, and tocotrienols. Understanding the biological activity of these phytochemicals at cellular level is crucial, with cellular antioxidant activity (CAA) being a more precise indicator of behavior in biological systems. However, the degradation of these compounds in the presence of oxygen and light challenges stability, driving nanoencapsulation as a solution. This technology not only enhances stability and controls release but also facilitates incorporation into foods. Nevertheless, the biological safety of nanoparticles in epithelial cells and their cytotoxicity remains relatively unexplored. Simultaneously, there is a growing interest in biopolymers and vegetable by-product flours as encapsulants, given their nutritional and bioactive composition. In this context, this proposal aimed to nanoencapsulate HCPO Unaué HIE OxG with vegetable by-products, covering the optimization of formulations, characterization of resulting nanoparticles, and evaluation of cellular antioxidant activity and cytotoxicity. Nanoparticles of HCPO with jackfruit seed flour (N-JSF) and nanoparticles of HCPO with jackfruit axis flour (N-JAF) were optimized using a 22 experimental design. The amount of HCPO and encapsulants were the tested independent variables, and particle size and polydispersity index (PDI) were the experimental responses. The selected formulations (mass ratio 0.8:1; JSF or JAF: HCPO) underwent characterization for zeta potential (ZP), apparent viscosity, pH, color parameters, and total carotenoids. Differentiated human colorectal adenocarcinoma cells (Caco-2) were employed to evaluate CAA at various concentrations (2.5; 50; 100; 150; 200; 250 µg/mL) for N-JSF, N-JAF, and free HCPO. Results were expressed in cellular antioxidant activity units (CAA units). Cytotoxicity was assessed through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay at the same concentrations, and relative cell viability (%) was calculated. The nanoparticles exhibited nanoscale diameters (<250 nm), monodisperse distribution, good uniformity, and stability (PDI < 0.25; ZP between +30 mV and -30 mV), high encapsulation efficiency (%) (N-JSF 86.44 ± 0.01 and N-JAF 90.43 ± 1.34), and optimal carotenoid retention (>85%). The average apparent viscosity was 21.89 ± 1.20 cP, and both formulations had a more acidic pH than basic. The nanoparticles showed a higher tendency toward yellow (higher b* values) than red, with an average L* value of 40.52 ± 2.97. CAA of N-JSF and N-JAF surpassed that of free HCPO at all concentrations studied for differentiated human colorectal adenocarcinoma cells (Caco-2), and none of the nanoparticles exhibited toxic effects on the cells. Concentrations of 150, 200, and 250 µg/mL for N-JSF and N-JAF, respectively, increased cell viability, indicating a proliferative effect on intestinal epithelial cells. In conclusion, nanoparticles of HCPO with vegetable by-products as encapsulants demonstrated satisfactory results, serving as a sustainable, clean label, and innovative alternative in oil encapsulation for preserving bioactive compounds and applying HCPO in the food industry. Additionally, the findings emphasize the importance of studying CAA in nanoparticles of oil rich in bioactives across different cell lines. The non-cytotoxic nature of nanoparticles indicates promising results regarding biological safety and the ability to prevent damage to intestinal epithelial cells at the studied concentrations.

COMMITTEE MEMBERS:
Presidente - 3170723 - CAMILA DUARTE FERREIRA RIBEIRO
Interno - 1980101 - MARCELO ANDRES UMSZA GUEZ
Interno - 2091662 - EDERLAN DE SOUZA FERREIRA
Externa à Instituição - LETICIA DE ALENCAR PEREIRA RODRIGUES - Senai
Externa à Instituição - BRUNA APARECIDA SOUZA MACHADO - Senai