Banca de DEFESA: RAQUEL NUNES ALMEIDA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAQUEL NUNES ALMEIDA DA SILVA
DATE: 12/12/2022
TIME: 14:00
LOCAL: Faculdade de Farmácia/Defesa remota
TITLE:

BIODIVERSITY OF CULTIVABLE YEASTS ASSOCIATED WITH HONEY AND POLLEN OF STINGLESS BEES: Identification by mass spectrometry MALDI-TOF MS

KEY WORDS:

Stingless bee. Honey. Pollen. Physical-chemical parameters. Yeasts. Maldi-Tof.

PAGES: 132
BIG AREA: Ciências Agrárias
AREA: Ciência e Tecnologia de Alimentos
SUMMARY:

There are more than 500 species and 61 genera of stingless bees known worldwide, distributed in tropical America, Africa, Southeast Asia and Australia. Meliponines store food (pollen and nectar) in pots, aiming to ensure the survival of the colony during resource scarcity events. Pollen is the main source of proteins, lipids and vitamins for bees; nectar represents the main source of carbohydrates. The sensory, physical and chemical characteristics of honey and pollen from stingless bees are quite different when compared to that produced by Apis melífera bees. The work is presented in three chapters, organized as follows: Chapter I presents the Literature Review that aims to contextualize aspects related to stingless bees; the honey; the pollen; the physical-chemical characteristics and the microbiological quality of the products; the interaction of bacteria, fungi and yeasts; as well as addresses the MALDI-TOF mass spectrometry method; Chapter II presents the article “Stingless bees and the physical-chemical and microbiological characteristics of honey and pollen: Literature Review” which aims to briefly report some data presented in the Literature Review; and Chapter III presents the article “Diversity of yeasts in samples of honey and pollen from stingless bees in the state of Bahia: Use of the MALDI-TOF technique” which aims to identify, through MALDI-TOF mass spectrometry, the species of yeasts from honey and bee pollen Melipona scutellaris, Nannotrigona testaceicornes and Tetragonisca angustula. The data obtained from the Literature Review show that meliponine honey has a high moisture content ranging from 23 to 37,5%; total acidity 19,9 to 98,43 meq kg-1; and reducing sugars 43 to 75,5%. The pollen has a high moisture content ranging from 37,12 to 53,93%; proteins 19,7 to 37,63%; lipids 2,5 to 10,81%; and fiber from 9,3 to 13,65%. The bacteria most associated with stingless bees are Lactobacillus, Bacillus, Streptomyces, Clostridium, Staphylococcus, Streptococcus, Enterobacter, Ralstonia, Pantoea, Pseudomonas, Fructobacillus, Lysinibacillus and Neisseria. The most common yeasts observed in honey are Metschnikowia, Starmerella, Zygosaccharomyces, Candida, Debaryomyces, Dekkera, Kloeckera and Pichia. And the fungal filaments Aspergillus, Penicillium and Talaromyces. In pollen, the presence of bacteria such as Lactobacillus, Bifidobacterium and Bacillus can be observed; yeasts such as Starmerella and Zigosaccharomyces osmophilus, and the fungi Penicillium and Talaromyces. The article presented in Chapter III identifies nine yeast species: Candida maltosa, Candida norvegica, Kazachstania telluris, Schizosaccharomyces pombe, Candida shehatae var. insectosa, Candida guilliermondii, Brettanomyces bruxelensis, Kazachstania exigua, Candida lactis-condensi associated with honey and bee pollen Melipona scutellaris, Nannotrigona testaceicornes and Tetragonisca angustula. The development of new research on the physicochemical characteristics of honey and pollen from stingless bees can contribute to the construction of specific legislation that defines the identity and quality standards of these products; the identification of new species contributes to the knowledge of new species associated with products, as well as for biotechnological application

COMMITTEE MEMBERS:
Presidente - 1980101 - MARCELO ANDRES UMSZA GUEZ
Externo ao Programa - 1561789 - RICARDO WAGNER DIAS PORTELA - UFBAExterna à Instituição - LETICIA DE ALENCAR PEREIRA RODRIGUES - Senai