Use of physical, chemical and biological parameters for the assessment of the environmetal conditions for municipal solid waste degradation in landfills
Landfill, Municipal Solid Waste, Anaerobic Digestion, Microorganisms, Metagenomic
The landfills are engineering equipment for the disposal of solid urban waste (MSW) for environmental protection and public health, modernly regarded as bioreactors, where anaerobic digestion microbial activities play a crucial role in their behavior and biogas generation. with energy potential. In this work molecular techniques (metagenomics) were used to test the hypothesis that an eventual oxygen entry into the massif cells through the cover layer was interfering in the composition, diversity and microbial structure of the MSW disposed at the Metropolitan Sanitary Landfill Center (MSLC).in the Metropolitan Region of Salvador (MRS), in Bahia. The results of metagenomic analysis demonstrated that high throughput sequencing of 16S rDNA can provide a comprehensive overview of the structure and diversity of prokaryotic communities and the pattern of group distribution in the MSW decomposition environment. In the present case, taxonomic analyzes indicated that Phyla Firmicutes (41.3%), Chloroflexi (10.9%), Actinobacteria (9.6%), Proteobacteria (7.1%), Bacteroidetes (6.7%) and Euryarchaeota (16.8%) are the most abundant microbial taxa from MSW ever grounded in the areas surrounding the MSLC DG 266 high suction gas drain, with Firmicutes being the most prevalent. The microbial groups found in the landfill fit well with the classic pathways of anaerobic digestion processes. Most of the microbial genera identified were anaerobic and superior to the aerobic genera. Despite the predominance of anaerobic genera, the use of the Hellinger distance PCA statistical technique showed the difference between samples with different distances / depths (p = 0.0001 and p = 0.0025, respectively), with distance being the most important variable in the study. The most abundant genera of microorganisms found were Methanobacterium, Methanosarcina, Methanofollis, Desulfosporosinus, Anaerolinea and Clostridium, all anaerobic. The results corroborate the hypothesis of oxygen inflow into the MSLC already grounded MSW massif by the cover layer, which changes the decomposition environment and leads to a drop in methane production. The variable with the highest correlation coefficient was distance, followed by Eh and depth. Correlation and PCA analyzes showed that the microbial diversity in the landfill was affected by the distance and depth of grounding.