Banca de DEFESA: CARLA JAQUELINE SILVA SAMPAIO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : CARLA JAQUELINE SILVA SAMPAIO
DATA : 29/04/2019
HORA: 14:00
LOCAL: CIENAM- Centro Interdisciplinar em Energia e Ambiente - UFBA /Sala 02
TÍTULO:

Bioremediation of hydrocarbons using encapsulated bacterial cells

PALAVRAS-CHAVES:

Consortium, Immobilization, Polymeric matrix, Petroleum, Bioremediation

PÁGINAS: 192
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
ESPECIALIDADE: Microbiologia Industrial e de Fermentação
RESUMO:

Oil is an important source of energy in the world. The petroleum exploration, refining, and transport are subject to environmental accidents that contaminate water, soil and constitute a risk to the health of man and other living beings. In this scenario, the improvement of the bioremediation techniques is fundamental for optimization of the decontamination of these environments. The present work aimed to select bacterial strains that degrade hydrocarbons and to develop a polymeric matrix for cellular encapsulation and bioremediation application. Degradation potential of 13 strains isolated from the gastrointestinal tract of animals of the Polychaeta class originated from the beaches of Todos os Santos Bay, with a history of hydrocarbon contamination, was evaluated. The selected strains were grouped in the consortium and tested, in the NPK presence, for the removal of n-alkanes, polycyclic aromatic hydrocarbons, and hopanes. The encapsulating polymer matrix was developed using 2% dental alginate and 0.5% and 1% corn starch and was evaluated for potential immobilization and bioremediation application. The results demonstrate that the bacteria of the consortium Acinetobacter sp., Bacillus sp., Enterobacter sp. and Pantoea sp. are effective in the metabolization of hydrocarbons, including when NPK has been added. The matrix developed is resistant to lyophilization, is porous and is stable for 120 days at 4 ° C in distilled water and glycerol. The matrix also demonstrated good cellular immobilization ability and maintained favorable conditions for bacterial growth, increasing basal respiration, bacterial biomass and removal of n-alkanes and PAHs from the sediment. High molecular weight n-alkanes obtained higher rates of degradation by immobilized cells. Of the 16 PAHs analyzed, only benzo (a) anthracene and anthracene were not degraded. Since naphthalene and dibenzo (a) anthracene reached degradation rates of 60% and 80%, respectively. The survey of patents on immobilization and bioremediation use has shown that this technique is an important field yet to be explored in the development of technological inventions. Therefore, the tested consortium and the developed polymer matrix are promising for use in bioremediation of environments contaminated by hydrocarbons.

MEMBROS DA BANCA:
Presidente - 1676978 - MILTON RICARDO DE ABREU ROQUE
Externo ao Programa - 1677204 - ELAINE CHRISTINE DE MAGALHAES CABRAL ALBUQUERQUE
Externo ao Programa - 3444827 - GILSON CORREIA DE CARVALHO
Externo ao Programa - 2322888 - OLIVIA MARIA CORDEIRO DE OLIVEIRA
Externo à Instituição - JUAN CARLOS ROSSI ALVA