Banca de DEFESA: LAIS FERRAZ BRITO SOUSA
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.STUDENT : LAIS FERRAZ BRITO SOUSA
DATE: 12/08/2022
TIME: 14:00
LOCAL: Sala 401 - Prédio Administrativo
TITLE:
Evaluation of the biological activities of the association of β-caryophyllene and docosahexaenoic acid in experimental models in vitro and in vivo.
KEY WORDS:
Methicillin-resistant Staphylococcus aureus. Inflammation. Nociception. Cancer. β-caryophyllene. Docosahexaenoic acid.
PAGES: 151
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
SPECIALTY: Microbiologia Médica
SUMMARY:
Introduction: Inflammation is a complex biological response involving the immune, autonomic, vascular and somatosensory systems, which involves the synthesis of inflammatory mediators and pain induction by activating nociceptors. One of the most common and impacting causes of inflammation in public health is infection by Staphylococcus aureus, the main cause of bacteremia, with worse in-hospital clinical outcomes. Antioxidant substances have been evaluated in this context as an analgesic, antioxidant, anti-inflammatory, antitumor, and bactericidal therapeutic alternatives. Among them, the essential oils of aromatic plants, such as β-caryophyllene (BCP), and polyunsaturated fatty acids, such as Docosahexaenoic acid (DHA) stand out. Objective: To evaluate the biological activities of the BCP-DHA association in vitro and in vivo experimental models of antinociception and inflammation. Materials and methods: For that, the antinociceptive effects were evaluated in animal models through tests of abdominal writhing induced by acetic acid, paw edema induced by intraplantar injection of formalin, and VonFrey hypernociception. To determine the anti-inflammatory effects, monocytes isolated from peripheral blood of adult male volunteers were infected with methicillin-resistant Staphylococcus aureus and incubated with treatment for subsequent cytokine measurement and gene expression analysis. The antioxidant capacity was evaluated by the DPPH method. The MTT assay was performed to determine the cell viability of the A549 and BEAS lung lines against treatment with BCP-DHA and then, adhesion and migration tests were performed with the doses of the associations determined in the previous test. Results: Antinociceptive effects were observed in the three models performed when comparing the control (saline) and BCP-DHA treated groups, obtaining statistical significance (p < 0.05) in the test of abdominal writhing induced by acetic acid, in the evaluation of flinches and Paw licking in the intraplantar formalin injection model and in Von Frey test hypernociception. There was a significant reduction in the levels of GM-CSF, TNFα, IL-1, IL-6, and IL-12, as well as an increase in IL-10 in the groups submitted to treatment with the BCP-DHA association, as well as negative regulation of the expression of genes involved in the intracellular inflammatory signaling cascade (IL-2, IL-6, IRF7, NLRP3, and TYK2) in all groups that received treatment, regardless of the presence of infection. In the DPPH test, only BCP showed significant scavenging of free radicals, with EC 50 = 2.82 ± 0.06 M. There was the significant cytotoxic activity of the 2 combinations of BCP-DHA tested on the A549 tumor cell line, as well as important inhibition of cell adhesion and migration. There was underexpression of genes involved in the cell cycle, DNA repair, nucleotide excision, mismatched bases and post replication, and maintenance of telomeres, oncogenes, and tumor suppressors. Therefore, our results strongly suggest that the association of BCP-DHA emerges as a possible new therapeutic alternative with analgesic, anti-inflammatory, antiangiogenic, pro-apoptotic, and antiproliferative effects.
BANKING MEMBERS:
Presidente - 1561874 - LUCAS MIRANDA MARQUES
Interna - 1553274 - REGIANE YATSUDA
Externa ao Programa - 1071194 - DAIANA SILVA LOPES
Externo ao Programa - 2315391 - FABRICIO FREIRE DE MELO
Externa à Instituição - HELLEN BRAGA MARTINS - UFBA