Banca de DEFESA: GRAZIELLE PRATES LOURENCO DOS SANTOS BITTENCOURT

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : GRAZIELLE PRATES LOURENCO DOS SANTOS BITTENCOURT
DATE: 01/08/2022
TIME: 08:00
LOCAL: Instituto Multidisciplinar em Saúde-UFBA (remoto)
TITLE:

Effects of high-fat diets with coconut or linseed oil carbohydrate-restricted and exercise on metabolic and hepatic parameters in obese rats.

KEY WORDS:

 Coconut oil. Linseed oil. Aerobic exercise. Hepatic steatosis.
Inflammation.

PAGES: 133
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Metabolismo e Bioenergética
SUMMARY:

Objective: To evaluate the effects of high-fat diets with coconut or linseed oil carbohydrate-restricted and moderate-intensity exercise on metabolic and hepatic parameters in obese rats. Methods: Fifty-four male Wistar rats were used and initially received a control (n=6) or hypercaloric (n=48) diet for 8 weeks to induce obesity. After this period, the animals of the control group (n=6) and part of the hypercaloric group (n=6) were euthanized and the others were subdivided into six groups, with different diets and submitted or not to moderate-intensity physical exercise for 12 weeks: hypercaloric and sedentary (n=7); hypercaloric and exercised (n=7); high-fat diet with coconut oil carbohydrate-restricted and sedentary (n=7); high-fat diet with coconut oil carbohydrate-restricted and exercised (n=7), high-fat diet with linseed oil
carbohydrate-restricted and sedentary (n=7) and high-fat diet with linseed oil carbohydrate-restricted and exercised (n=7). Dietary, cardiovascular, and body composition parameters were evaluated, as well as lipid and plasma inflammatory profile, glycemic homeostasis, insulin resistance and sensitivity. In addition, the development of steatosis, inflammation, fibrosis, and oxidative stress in liver were evaluated. Results: The experimental model of diet-induced obesity was validated. The high-fat diet with coconut oil carbohydrate-restricted reduced food and caloric intake, but increased body adiposity, blood pressure, glucose and insulin levels, reduced IL-10 levels, increased liver cholesterol, promoted macrovesicular fat deposition, inflammation, fibrosis and oxidative stress in liver tissue. The high-fat diet with linseed oil carbohydrate-restricted, although it increased caloric intake, did not increase body mass and adiposity, improved lipid profile, reduced IL6 and IL-10 levels, oxidative stress and the deposition of macro and microvesicles of fat in the liver, however, promoted hepatic inflammation and hyperglycemia. Exercise enabled beneficial effects on most of the metabolic and hepatic parameters analyzed. Conclusion: Consumption of the high-fat diet with coconut oil carbohydrate-restricted
negatively impacted body adiposity and systemic and hepatic metabolic and inflammatory profiles, while consumption of the high-fat diet with linseed oil carbohydrate-restricted and exercise had beneficial effects on most of the metabolic and hepatic parameters analyzed. Thus, caution is suggested in the use of these diets, even when associated with exercise, reinforcing the need for further studies to determine safety recommendations for including these oils in the diet.

BANKING MEMBERS:
Presidente - 1112111 - AMELIA CRISTINA MENDES DE MAGALHAES GUSMAO
Interno - 2497915 - ROBSON AMARO AUGUSTO DA SILVA
Externo ao Programa - 2927260 - MATHEUS LOPES CORTES
Externo à Instituição - RAILDO DA SILVA COQUEIRO - UESB
Externo à Instituição - RAFAEL PEREIRA DE PAULA - UESB