Banca de DEFESA: DANILO NASCIMENTO LOPES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANILO NASCIMENTO LOPES
DATA : 15/12/2020
HORA: 14:00
LOCAL: Instituto Multidisciplinar em Saúde
TÍTULO:

Effect of preventive exercise on pancreatic changes induced by type 1 diabetes mellitus in ovariectomized rats.

PALAVRAS-CHAVES:

Diabetes mellitus, pancreas, ovariectomy, physical exercise, oxidative stress, inflammation.

PÁGINAS: 78
GRANDE ÁREA: Ciências Biológicas
ÁREA: Fisiologia
SUBÁREA: Fisiologia de Órgãos e Sistemas
ESPECIALIDADE: Fisiologia Endócrina
RESUMO:

One of the main changes in type 1 diabetes mellitus (T1DM) is the progressive reduction of the pancreatic β cells, and consequently insulin production. Lipid peroxidation, pro-inflammatory cytokines, and nuclear transcription factor (NF-kB) play an important role in β cell dysfunction and death during T1DM. In addition, the transforming growth factor (TGF-β1) and fibrosis participate in the development of diabetes mellitus. The beneficial effects of physical exercise on complications in various organs resulting from DM have been demonstrated. However, there are no studies evaluating the effects of exercise on islet of langerhans of diabetic ovariectomized rats. For this, wistar rats were anesthetized and submitted to bilateral ovariectomy. After surgical recovery, the animals were injected with 40 mg/kg streptozotocin (STZ) for the induction of DM1 or 0.1M citrate buffer, pH 4.5, and were divided into four experimental groups: 6 sedentary control rats OVX (SCO); 6 previously trained OVX controls (PTCO); 6 sedentary diabetic OVX (SDO); and 6 previously trained diabetic OVX (PTDO). The animals of the PTDO and PTCO groups were submetted four weeks of aerobic training previous to induction of T1DM. After four weeks, T1DM induction was performed in the animals of the SDO and PTDO groups. Then, the PTCO and PTDO groups were submitted to the same protocol of regular aerobic physical exercise of moderate intensity, which consisted of running on a motorized treadmill for a period of eight weeks. Physical exercise did not increase the insulin content and mass of β cells in the islets of langerhans in the PTDO group. However, it decreased the content of glucagon and α cell mass, showing the beneficial effect of exercise on the counter-regulating hormone of blood glucose homeostasis. In addition, blood glucose from PTDO rats was lower than that of SDO rats. Physical exercise also decreased TGF-β1 expression in PTDO rats compared to SCO rats, as well as decreased fibrosis in these islets. Physical exercise reduced nitrative stress by reduced nitrotyrosine expression in the PTDO group. In addition, physical exercise had a fundamental effect in reducing the expression of the transcription factor NF-kB. These results show the beneficial effect of physical exercise prior to the induction of diabetes on the expression of inflammatory and oxidative markers, islet structure and content of the counter-regulating hormone of blood glucose concentration.

MEMBROS DA BANCA:
Presidente - 1552395 - TELMA DE JESUS SOARES
Interno - 2312625 - LILIANY SOUZA DE BRITO AMARAL
Externo ao Programa - 2418292 - SAMIRA ITANA DE SOUZA