Banca de DEFESA: MANOELA RIOS TRINDADE CARNEIRO
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.DISCENTE : MANOELA RIOS TRINDADE CARNEIRO
DATA : 11/12/2020
HORA: 14:00
LOCAL: Instituto Multidisciplinar em Saúde
TÍTULO:
Evaluation of galactagogue activity of ethanol extract, fractions and a compound isolated from Pimpinella anisum Lineu seeds in an experimental model with rats.
PALAVRAS-CHAVES:
Lactation. Medicinal plants. Galactagogues. Anise. Pimpinella anisum L.
PÁGINAS: 131
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
RESUMO:
Pimpinella anisum Lineu is used to increase the production of breast milk, but is empirically used by mothers with hypogalactia, and its effectiveness, safety, effective dose and mechanisms of action must be proven. This study evaluated the phytochemical profile and galactagogue activity of the ethanolic extract (EEPA), fractions of the seeds of P. anisum and the isolated compound anethole, as well as the toxicity of the EEPA. Lactating Wistar rats breastfeeding seven to eight puppies each, were divided into groups treated daily intraperitoneally with (n = 6): a) 0.4 ml of distilled water; b) EEPA in doses of 12.5; 25 and 50 mg/kg; c) FH doses at 12.5; 25 and 50 mg/kg; d) FD in doses of 12.5; 25 and 50 mg/Kg, e) FAE at doses of 12.5; 25 and 50 mg/kg; f) FB at doses of 6.25; 12.5 and 25 mg/kg; and only g) anethole in doses of 12.5; 25 and 50 mg/kg; orally. The daily and total weight gain of the litter was determined, the estimated milk production in 1 h of breastfeeding, the weight loss of the lactating rat and the breastfeeding time from the 4th to the 18th day of life of the puppies. On the 19th day, rats were milked to determine the energy content and the major constituents of milk, being euthanized on the 20th day and removed the mammary glands for histological analysis. In the 2nd experiment, the prolactin and oxytocin diets were measured in the adenohypophysis or neurohypophysis, serum and mammary gland in the rats on the 14th and 21st day of lactation, divided into the following groups (n = 6): a) 0.4 mL distilled water, b) EEPA (12.5 mg/kg) and c) anethole (50 mg/kg). Toxicity to Artemia salina (12.5; 25; 50, 100 and 200 μg/mL) and acute toxicity with determination of LD50 with male and specific Wistar rats were analyzed for groups (n: 3): a) ethanol 10%, b) 5, 50, 300 and 2000 mg/kg of EEPA; and c) 0.4 ml distilled water. Galactagogue activity was observed only with EEPA (12.5 mg/kg), fraction of dichloromethane (25 mg/kg) and anethole (50 mg/kg), as there was greater daily weight gain (24.8%, 11, 6%, 10.2%) and total (28.1%, 11.37%, 9.7%), and estimated milk production (32.6%, 40%, 22.4%) (p < 0.05), without changes in the weight loss of the lactating rat and without breastfeeding time (p> 0.05) in relation to the control. EEPA increased the concentration of total proteins and cholesterol (84.7% and 185%), FD increased the concentration of lactose, total lipids and cholesterol (27.6%, 140.4% and 140%) and anethole increased all constituents in relation to the control (p <0.05). Although the mammary glands were histologically in process of involution, the smaller number of lobes and alveoli of the EEPA and the smaller number of alveoli of the FD and anethole indicate that they were distended and the gland was more active previously. The highest levels of adenohypophyseal, serum and mammary gland prolactin at 21 days were found with EEPA and anethole (p <0.05). Increased oxytocin concentration occurred on the 14th day in the neurohypophysis, and in the EEPA serum and only in the anethole neurohypophysis. EEPA has no acute toxicity or animal death. Thus, as seeds of P. anisum have galactagogue activity attributed to the presence of compounds with estrogenic activity (β-sitosterol, stigmasterol, anethole and their polymers) and dopamine antagonists (anethole) that stimulate a greater production of prolactin, when it comes to histological changes of the mammary gland, constituents and quantity of milk, reflecting the greater growth of the litter.
MEMBROS DA BANCA:
Presidente - 1553274 - REGIANE YATSUDA
Interno - 1112111 - AMELIA CRISTINA MENDES DE MAGALHAES GUSMAO
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo ao Programa - 1071194 - DAIANA SILVA LOPES
Externo à Instituição - ÉRIKA PEREIRA DE SOUZA - FTC